Detailed information NTG0458532

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: I6J15_RS00290
• Genome accession: NZ_CP068056
• Coordinates: 63986..64666 (-)
• Length: 226 a.a.
• NCBI ID: WP_020916423.1
• Uniprot ID: A0AB33AKS4
• Organism: Streptococcus lutetiensis strain FDAARGOS_1158
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 58986..69666

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6J15_RS00270 (I6J15_00270)	-	59127..59852 (-)	726	WP_043894998.1	YebC/PmpR family DNA-binding transcriptional regulator	-
I6J15_RS00275 (I6J15_00275)	-	59954..60565 (-)	612	WP_020916426.1	superoxide dismutase	-
I6J15_RS00280 (I6J15_00280)	holA	60660..61697 (-)	1038	WP_020916425.1	DNA polymerase III subunit delta	-
I6J15_RS00285 (I6J15_00285)	-	61762..63993 (-)	2232	Protein_56	DNA internalization-related competence protein ComEC/Rec2	-
I6J15_RS00290 (I6J15_00290)	comEA	63986..64666 (-)	681	WP_020916423.1	helix-hairpin-helix domain-containing protein	Machinery gene
I6J15_RS00295 (I6J15_00295)	-	64763..65509 (-)	747	WP_111712677.1	lysophospholipid acyltransferase family protein	-
I6J15_RS00300 (I6J15_00300)	-	65625..66386 (+)	762	WP_111712676.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
I6J15_RS00305 (I6J15_00305)	-	66361..66636 (+)	276	WP_111712675.1	GIY-YIG nuclease family protein	-
I6J15_RS00310 (I6J15_00310)	-	66677..68254 (-)	1578	WP_020916419.1	DEAD/DEAH box helicase	-

Sequence

Protein

• Length: 226 a.a.
• Molecular weight: 23950.98 Da
• Isoelectric Point: 4.8421

>NTDB_id=458532 I6J15_RS00290 WP_020916423.1 63986..64666(-) (comEA) [Streptococcus lutetiensis strain FDAARGOS_1158]
MIEEMKERLLEHKTVAAVLGTVLVMLVVFFAWSSVTRPQAQLQSDLPTISSSIPASSSLETTKIKTMTSHSQDDSGKIFV
DIKGAVKNEGVYELSNGSRVTDLVKKAGGFTDDADKKSVNLAEKLADEAVVYVAKIGEEVTPASTLSQAKPTTARGGQGD
GSAQINLNTATTEELQTISGIGAKRAQDIIDYRDANGGFSSVGDLKNVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 681 bp

>NTDB_id=458532 I6J15_RS00290 WP_020916423.1 63986..64666(-) (comEA) [Streptococcus lutetiensis strain FDAARGOS_1158]
ATGATTGAAGAGATGAAAGAAAGACTTTTGGAACATAAGACGGTAGCAGCGGTTTTAGGAACGGTGCTTGTTATGCTTGT
GGTATTCTTTGCTTGGTCTAGTGTGACCAGACCACAAGCTCAGCTACAAAGTGACTTACCAACAATTTCTAGTAGTATCC
CAGCAAGTTCAAGTTTAGAAACAACAAAAATAAAGACTATGACATCTCATTCTCAAGATGACTCTGGCAAGATTTTTGTC
GATATCAAAGGTGCTGTTAAAAATGAGGGTGTTTATGAACTGTCAAATGGCAGCCGTGTTACGGATTTAGTCAAAAAAGC
TGGTGGATTTACAGATGATGCTGATAAGAAGTCAGTCAATTTAGCTGAAAAATTAGCTGACGAAGCGGTTGTCTATGTGG
CAAAAATTGGTGAAGAAGTCACACCAGCAAGCACACTTTCTCAAGCTAAACCAACGACAGCTAGGGGGGGACAAGGTGAC
GGCTCAGCGCAAATCAATCTTAACACCGCAACTACAGAAGAACTCCAAACTATTTCTGGCATTGGGGCTAAACGTGCGCA
AGATATCATTGATTATCGTGATGCTAATGGTGGTTTCTCATCTGTTGGTGATTTGAAAAATGTTTCTGGCATTGGTGAGA
AGACTTTGGAAAAATTAAAATCTGAGGTGACTGTTGATTAA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (163-224)

• SLBB domain (SLBB) (80-133)

3D structure

• AlphaFold DB: A0AB33AKS4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	44.068	100	0.46
comEA/celA/cilE	Streptococcus mitis SK321	44.934	100	0.451
comEA/celA/cilE	Streptococcus mitis NCTC 12261	44.493	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae D39	42.735	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	42.735	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae R6	42.735	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.612	100	0.438
comEA	Lactococcus lactis subsp. cremoris KW2	39.111	99.558	0.389
comEA	Bacillus subtilis subsp. subtilis str. 168	39.269	96.903	0.381
comEA	Latilactobacillus sakei subsp. sakei 23K	37.5	99.115	0.372