Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWV00_RS19415 Genome accession   NZ_CP056123
Coordinates   4351393..4353096 (+) Length   567 a.a.
NCBI ID   WP_211683888.1    Uniprot ID   -
Organism   Moritella sp. 24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4346393..4358096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV00_RS19385 (HWV00_19385) pdhR 4346433..4347194 (-) 762 WP_211683877.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HWV00_RS19390 (HWV00_19390) ampE 4347552..4348400 (-) 849 WP_211683879.1 regulatory signaling modulator protein AmpE -
  HWV00_RS19395 (HWV00_19395) ampD 4348402..4348965 (-) 564 WP_211683880.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HWV00_RS19400 (HWV00_19400) - 4349119..4349625 (+) 507 WP_211683882.1 TIGR02281 family clan AA aspartic protease -
  HWV00_RS19405 (HWV00_19405) nadC 4349650..4350492 (+) 843 WP_211683884.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HWV00_RS19410 (HWV00_19410) - 4350928..4351386 (+) 459 WP_211683886.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HWV00_RS19415 (HWV00_19415) pilB 4351393..4353096 (+) 1704 WP_211683888.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWV00_RS19420 (HWV00_19420) pilC 4353163..4354416 (+) 1254 WP_211683890.1 type II secretion system F family protein Machinery gene
  HWV00_RS19425 (HWV00_19425) pilD 4354536..4355423 (+) 888 WP_211683892.1 A24 family peptidase Machinery gene
  HWV00_RS19430 (HWV00_19430) coaE 4355435..4356040 (+) 606 WP_211683894.1 dephospho-CoA kinase -
  HWV00_RS19435 (HWV00_19435) yacG 4356068..4356259 (+) 192 WP_211686713.1 DNA gyrase inhibitor YacG -
  HWV00_RS19440 (HWV00_19440) mutT 4356292..4356684 (-) 393 WP_211683896.1 8-oxo-dGTP diphosphatase MutT -
  HWV00_RS21660 - 4356825..4356911 (-) 87 Protein_3733 SEC-C metal-binding domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62927.04 Da        Isoelectric Point: 5.0499

>NTDB_id=458297 HWV00_RS19415 WP_211683888.1 4351393..4353096(+) (pilB) [Moritella sp. 24]
MQHKHNASGLAHSLVAHSYLATEEIPSLLQSAKQEGKAFTSYLVDNNIIDSHTLAQLLEREYGVPLLDLDTFKLEEIPDN
LLNEKLIDKHHALPIYVQGQTLYLAMSDPTNVTALEEFSFSFSLHTEVLLVDEIQLQKALEIVLESDMDALGDLNDADIG
DLEVDNSESRLDQENKDSADDAPIVKFVNKILLDAIKRGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAKLDIAERRIPQDGRIKLKLSKNKSVDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDEKQKALYLKALERPQG
MILVTGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLTGINQVQINLKAGLTFPSALRSFLRQDPDVVMVGEIRDI
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCNECKQPEEIPSVELVKLGFSP
QQINQGITPFKAIGCNQCTKGYKGRVGIYEVMPMSDTIARMILTGSNSMELNDQAQQEGMDTLRQSALQRVIDGVTSLLE
VERITSH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=458297 HWV00_RS19415 WP_211683888.1 4351393..4353096(+) (pilB) [Moritella sp. 24]
ATGCAACACAAGCATAACGCAAGCGGCCTAGCGCATAGCCTGGTCGCACATTCGTACCTCGCGACAGAAGAGATCCCATC
ACTTCTGCAAAGCGCCAAACAGGAAGGTAAAGCCTTCACAAGCTATCTCGTCGATAATAACATCATTGATAGCCACACAT
TAGCGCAATTATTAGAGCGAGAGTACGGCGTCCCTCTACTCGATTTAGATACTTTCAAGCTCGAAGAAATACCCGATAAT
CTCCTTAATGAAAAACTGATTGATAAGCACCATGCATTGCCTATTTATGTGCAAGGGCAAACACTGTATCTCGCGATGTC
AGATCCAACGAACGTAACGGCCTTAGAAGAGTTCTCTTTCAGCTTTAGTCTGCATACCGAAGTGCTTTTAGTTGATGAAA
TACAATTACAAAAAGCCTTAGAGATTGTTCTCGAAAGTGATATGGATGCCCTTGGCGATCTGAATGATGCCGACATTGGT
GATTTAGAGGTTGATAATAGCGAGTCACGCTTAGATCAAGAAAACAAAGATAGCGCTGACGATGCTCCGATTGTTAAATT
CGTCAACAAGATCCTACTCGATGCAATCAAAAGGGGTGCCTCAGATATTCATTTCGAACCCTACGAATTTAAATACCGAG
TACGATTTCGGATCGACGGTATTTTACACGAAATGGTATCGCCTCCAGTTAATTTAGCCATGCGCTTTTCAGCTCGATTA
AAAGTAATGGCAAAACTGGATATTGCCGAACGTCGTATTCCCCAAGATGGTCGGATCAAGTTAAAACTTTCCAAAAATAA
ATCCGTTGATCTCCGTGTTAGTACCTTGCCCACCATGTGGGGAGAGAAAGTCGTTATGCGGATCTTGGATTCGAATCAAG
CCAGTTTAAACATTGATATTCTCGGCTATGATGAAAAACAAAAAGCACTTTACCTTAAAGCATTAGAAAGACCGCAAGGT
ATGATCTTGGTTACTGGACCAACAGGTAGTGGTAAAACAGTGTCGTTATACAGTGGATTAAATATTCTCAATACTGTCGA
ACGCAACATATCCACAGCCGAAGATCCTATCGAGATCAACTTAACCGGTATCAATCAAGTACAGATCAACCTGAAAGCAG
GGCTTACTTTCCCCAGTGCATTGCGTTCTTTCTTACGACAAGATCCTGATGTGGTCATGGTTGGTGAGATCCGAGATATT
GAAACTGCTGAAATTGCGATCAAAGCAGCGCAAACCGGTCACTTGGTGTTATCAACACTGCATACGAACTCCGCAGCTGA
AACGCTAACCCGTTTATCTAATATGGGGGTTCCTGCTTATAATATTGGTTCATCCGTCAGCTTGATCATCGCCCAGCGTC
TTGGCCGACGACTTTGTAACGAATGCAAACAACCAGAGGAGATACCTTCGGTTGAATTAGTCAAACTCGGATTTAGCCCG
CAACAAATTAACCAAGGGATCACACCTTTCAAAGCCATAGGCTGTAATCAGTGCACTAAAGGTTATAAAGGCCGTGTGGG
TATCTACGAAGTAATGCCAATGTCTGACACGATCGCACGCATGATTTTAACAGGCAGTAATTCGATGGAGCTAAACGATC
AAGCACAACAAGAAGGCATGGATACATTACGCCAGTCTGCGTTACAGAGAGTGATCGATGGGGTAACAAGCTTACTTGAG
GTCGAGCGGATCACGAGTCATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.89

100

0.533
  pilB Acinetobacter baumannii D1279779

52.043

99.295

0.517
  pilB Acinetobacter baylyi ADP1

52.143

98.765

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

52.583

95.591

0.503
  pilB Vibrio cholerae strain A1552

51.277

96.649

0.496
  pilB Vibrio campbellii strain DS40M4

51.565

95.767

0.494
  pilF Neisseria gonorrhoeae MS11

47.5

98.765

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

37.847

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.646

99.647

0.395
  pilF Thermus thermophilus HB27

37.39

100

0.374