Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWV01_RS01370 Genome accession   NZ_CP056122
Coordinates   288457..290160 (-) Length   567 a.a.
NCBI ID   WP_211673737.1    Uniprot ID   -
Organism   Moritella sp. 5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 283457..295160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV01_RS01345 (HWV01_01340) mutT 284864..285256 (+) 393 WP_211675601.1 8-oxo-dGTP diphosphatase MutT -
  HWV01_RS01350 (HWV01_01345) yacG 285279..285470 (-) 192 WP_211675603.1 DNA gyrase inhibitor YacG -
  HWV01_RS01355 (HWV01_01350) coaE 285497..286102 (-) 606 WP_211673734.1 dephospho-CoA kinase -
  HWV01_RS01360 (HWV01_01355) pilD 286114..287001 (-) 888 WP_211673735.1 A24 family peptidase Machinery gene
  HWV01_RS01365 (HWV01_01360) pilC 287121..288371 (-) 1251 WP_211673736.1 type II secretion system F family protein Machinery gene
  HWV01_RS01370 (HWV01_01365) pilB 288457..290160 (-) 1704 WP_211673737.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWV01_RS01375 (HWV01_01370) - 290167..290580 (-) 414 WP_211673738.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HWV01_RS01380 (HWV01_01375) nadC 290969..291811 (-) 843 WP_211673739.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HWV01_RS01385 (HWV01_01380) - 291836..292342 (-) 507 WP_211673740.1 TIGR02281 family clan AA aspartic protease -
  HWV01_RS01390 (HWV01_01385) ampD 292487..293059 (+) 573 WP_211673741.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HWV01_RS01395 (HWV01_01390) ampE 293061..293909 (+) 849 WP_211673742.1 regulatory signaling modulator protein AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62758.85 Da        Isoelectric Point: 5.1170

>NTDB_id=458259 HWV01_RS01370 WP_211673737.1 288457..290160(-) (pilB) [Moritella sp. 5]
MPHKHNASGLAHSLVAHSYLATEEIQQLLQSAKQEGKAFTTYIVDNNIIDSKTLAQLLEREYGVPLLDLDTFKLEEIPDN
LLNGKLIEKHHALPIYVQGQTLYLAMSDPTNVTALEEFSFSFSLHTEVLLVDEVQLQKALEIVLESDIDALGDLNDASID
ELEVDNNESRLEQENNSGADDAPIVKFVNKILLDAIKKGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAQLDIAERRVPQDGRIKLKLSKNKSIDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDDTQKALYLKALEKPQG
MILITGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLTGINQVQINLKAGLNFPSALRSFLRQDPDVVMVGEIRDI
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCSECKQPEDIPAIELAKLGFSQ
QQINDGITSFKAIGCNQCTKGYKGRVGIYEVMPMSDKIARMILTGSNSLELSDQAQQEGMNTLRQSALKKVIDGVTSLLE
VERITNH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=458259 HWV01_RS01370 WP_211673737.1 288457..290160(-) (pilB) [Moritella sp. 5]
ATGCCACACAAGCATAATGCTAGCGGCCTGGCACATAGCCTGGTCGCACATTCATACCTCGCCACAGAAGAGATTCAACA
ACTTCTGCAAAGCGCTAAACAGGAAGGTAAAGCCTTCACGACTTATATCGTCGACAATAACATCATCGACAGTAAAACCT
TAGCGCAATTACTAGAGCGTGAATACGGCGTTCCACTACTCGATTTAGATACCTTTAAACTGGAAGAAATTCCCGATAAC
CTGCTTAATGGAAAATTAATCGAGAAACACCATGCTTTGCCTATCTACGTCCAGGGACAAACTTTATATCTGGCGATGTC
AGATCCAACCAATGTCACCGCATTAGAGGAGTTCTCTTTTAGCTTCAGTCTGCATACCGAAGTACTTTTGGTTGATGAAG
TACAATTACAAAAAGCGTTAGAGATTGTACTCGAAAGTGATATTGATGCCCTTGGTGATCTGAATGATGCCAGCATTGAT
GAGTTAGAGGTTGATAACAACGAGTCACGCCTAGAGCAAGAAAATAACAGCGGTGCTGACGACGCCCCGATTGTTAAATT
CGTCAATAAGATCTTACTGGATGCGATTAAAAAAGGCGCATCCGATATTCACTTCGAACCGTATGAATTCAAATACCGGG
TGCGTTTTCGTATCGACGGTATCTTACATGAGATGGTATCTCCACCCGTTAACTTAGCGATGCGTTTTTCTGCGCGCTTA
AAAGTCATGGCGCAATTAGATATTGCCGAGCGTAGAGTGCCACAAGATGGCCGAATCAAATTAAAATTATCCAAAAACAA
GTCCATTGATCTACGCGTTAGCACCCTGCCCACCATGTGGGGTGAAAAGGTGGTGATGCGGATCTTAGATTCTAACCAAG
CCAGTCTCAACATTGATATTCTCGGTTATGACGATACACAAAAAGCCTTGTACCTTAAAGCGTTAGAAAAACCGCAGGGC
ATGATCTTGATCACAGGCCCAACAGGCAGTGGTAAAACGGTGTCTTTATACAGTGGCCTCAATATCCTCAACACCGTGGA
ACGCAACATATCCACAGCCGAAGATCCTATCGAGATCAACCTCACAGGTATCAATCAAGTCCAGATCAACCTGAAAGCAG
GACTGAACTTCCCCAGTGCATTGCGATCATTCTTACGTCAAGACCCGGATGTAGTCATGGTTGGTGAGATCCGCGATATA
GAAACAGCCGAGATTTCGATCAAAGCAGCGCAAACCGGTCACTTAGTATTATCTACTCTGCATACCAACTCTGCAGCAGA
AACCCTTACTCGTTTATCCAACATGGGTGTCCCTGCTTATAATATAGGTTCATCAGTCAGCTTGATCATCGCCCAACGGC
TCGGCCGTCGCCTTTGCAGTGAGTGTAAGCAGCCCGAAGACATACCCGCTATCGAATTAGCCAAGCTCGGTTTTAGCCAA
CAGCAAATCAATGATGGGATCACTTCATTTAAAGCCATAGGTTGTAACCAATGTACTAAAGGTTATAAAGGACGCGTAGG
GATCTACGAAGTCATGCCGATGTCAGATAAGATCGCACGTATGATCTTAACCGGCAGCAACTCGCTTGAACTCAGCGACC
AAGCGCAGCAAGAAGGCATGAATACATTACGCCAATCAGCCTTGAAAAAAGTGATCGACGGCGTCACCAGTTTACTTGAG
GTTGAACGTATCACTAATCATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-141)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.108

99.295

0.527
  pilB Legionella pneumophila strain ERS1305867

51.748

100

0.522
  pilB Acinetobacter baylyi ADP1

52.679

98.765

0.52
  pilB Vibrio cholerae strain A1552

51.92

96.473

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

98.942

0.499
  pilB Vibrio campbellii strain DS40M4

50.826

96.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.372

99.647

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

37.418

100

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.076

99.295

0.388
  pilF Thermus thermophilus HB27

37.3

99.295

0.37