Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWV02_RS01335 Genome accession   NZ_CP056121
Coordinates   283489..285192 (-) Length   567 a.a.
NCBI ID   WP_211689509.1    Uniprot ID   -
Organism   Moritella sp. 28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278489..290192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV02_RS01310 (HWV02_01310) mutT 279896..280288 (+) 393 WP_211679944.1 8-oxo-dGTP diphosphatase MutT -
  HWV02_RS01315 (HWV02_01315) yacG 280311..280502 (-) 192 WP_211675603.1 DNA gyrase inhibitor YacG -
  HWV02_RS01320 (HWV02_01320) coaE 280529..281134 (-) 606 WP_211689507.1 dephospho-CoA kinase -
  HWV02_RS01325 (HWV02_01325) pilD 281146..282033 (-) 888 WP_211678152.1 A24 family peptidase Machinery gene
  HWV02_RS01330 (HWV02_01330) pilC 282153..283403 (-) 1251 WP_211689508.1 type II secretion system F family protein Machinery gene
  HWV02_RS01335 (HWV02_01335) pilB 283489..285192 (-) 1704 WP_211689509.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWV02_RS22955 (HWV02_01340) - 285202..285636 (-) 435 WP_211678155.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HWV02_RS01345 (HWV02_01345) nadC 286027..286869 (-) 843 WP_211673739.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HWV02_RS01350 (HWV02_01350) - 286894..287400 (-) 507 WP_211678156.1 TIGR02281 family clan AA aspartic protease -
  HWV02_RS01355 (HWV02_01355) ampD 287545..288117 (+) 573 WP_211689510.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HWV02_RS01360 (HWV02_01360) ampE 288119..288967 (+) 849 WP_211689511.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62754.86 Da        Isoelectric Point: 5.0454

>NTDB_id=458235 HWV02_RS01335 WP_211689509.1 283489..285192(-) (pilB) [Moritella sp. 28]
MLHKHNAGGLAHSLVAHSYLVTEEIPQLLQSAKQEGKAFTSYLVDNNIIDSQVLAQLLEREYGVPLLDLDTFKLEEIPDN
LLNGKLIEKHHALPIYVQGQTLYLAMSDPTNVTALEEFSFSFSLHTEVLLVDEVQLQKALEIVLESDIDALGDLNDASID
ELEVDNNESRLEQENNSGADDAPIVKFVNKILLDAIKKGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAQLDIAERRVPQDGRIKLKLSKNKSIDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDDTQKALYLKALEKPQG
MILITGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLTGINQVQINQKAGLNFPSALRSFLRQDPDVVMVGEIRDI
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCSECKQPEDIPAIELAKLGFSQ
QQINDGITSFKAIGCNQCTKGYKGRVGIYEVMPMSDKIARMILTGSNSLELSEQAQQEGMNTLRQSALKKVIDGVTSLLE
VERITNH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=458235 HWV02_RS01335 WP_211689509.1 283489..285192(-) (pilB) [Moritella sp. 28]
ATGCTACACAAACATAACGCTGGCGGCCTGGCTCACAGCCTGGTCGCACATTCATACCTTGTAACAGAAGAAATTCCCCA
ACTTCTGCAAAGCGCCAAACAGGAAGGTAAAGCCTTCACAAGCTATCTCGTCGACAATAACATCATAGACAGCCAAGTAT
TAGCGCAATTACTAGAGCGTGAATATGGCGTTCCACTACTCGATTTAGATACCTTTAAACTGGAAGAAATTCCCGATAAC
CTGCTTAATGGAAAATTAATCGAAAAACACCATGCTCTGCCTATCTACGTGCAGGGACAAACATTATATCTGGCGATGTC
AGACCCGACCAATGTCACCGCATTAGAAGAGTTTTCCTTTAGCTTCAGTTTGCATACCGAAGTACTTTTGGTTGATGAAG
TACAATTACAAAAAGCGTTAGAGATAGTACTCGAAAGTGATATTGATGCCCTTGGTGATCTGAATGATGCCAGCATTGAT
GAGTTAGAAGTTGATAACAACGAGTCACGCCTGGAGCAAGAAAATAACAGCGGTGCTGACGACGCCCCGATTGTTAAATT
CGTCAATAAGATCTTACTGGATGCGATTAAAAAAGGCGCATCCGATATTCACTTCGAGCCCTATGAATTCAAATACCGGG
TACGTTTTCGTATCGACGGTATCTTACATGAGATGGTATCGCCACCCGTAAACTTAGCGATGCGTTTTTCTGCGCGCTTA
AAAGTCATGGCGCAATTAGATATTGCCGAGCGTAGAGTGCCACAAGATGGCCGAATCAAATTAAAATTATCCAAAAACAA
GTCCATTGATCTACGTGTTAGCACCCTGCCCACCATGTGGGGTGAAAAAGTGGTGATGCGGATCTTAGATTCTAACCAAG
CCAGTCTCAACATTGATATTCTCGGTTATGACGATACACAAAAAGCGCTGTACCTTAAAGCGTTAGAAAAACCGCAGGGC
ATGATCTTGATCACAGGCCCAACAGGCAGTGGTAAAACGGTGTCTTTATACAGTGGCCTCAATATCCTCAACACCGTGGA
ACGCAACATATCCACAGCCGAAGATCCTATCGAGATCAACCTCACAGGTATCAATCAAGTCCAAATCAACCAGAAAGCAG
GACTGAACTTCCCCAGTGCATTGCGATCATTCTTACGCCAAGACCCGGATGTTGTCATGGTTGGTGAGATCCGCGATATA
GAAACAGCCGAGATTTCGATCAAAGCAGCGCAAACCGGTCACTTAGTATTATCGACTCTGCATACCAACTCTGCAGCAGA
AACCCTCACTCGTTTATCCAACATGGGTGTCCCTGCTTATAATATAGGTTCATCAGTCAGCTTGATCATCGCCCAACGGC
TCGGCCGTCGCCTGTGCAGTGAGTGTAAGCAGCCCGAAGACATACCCGCTATCGAATTAGCCAAGCTCGGTTTTAGCCAA
CAGCAAATCAATGATGGGATCACTTCATTTAAAGCCATAGGTTGTAACCAATGTACTAAAGGTTATAAAGGACGCGTAGG
GATCTACGAAGTCATGCCGATGTCAGATAAGATCGCACGCATGATCTTAACCGGCAGCAACTCGCTTGAACTCAGCGAAC
AAGCGCAGCAAGAAGGCATGAATACATTACGCCAATCAGCCTTGAAAAAAGTGATCGACGGCGTGACCAGTTTACTTGAG
GTTGAGCGTATCACTAATCATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.203

99.118

0.527
  pilB Legionella pneumophila strain ERS1305867

52.12

99.824

0.52
  pilB Acinetobacter baylyi ADP1

52.415

98.589

0.517
  pilB Vibrio cholerae strain A1552

53.008

93.827

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

51.756

95.414

0.494
  pilB Vibrio campbellii strain DS40M4

51.197

95.767

0.49
  pilF Neisseria gonorrhoeae MS11

46.549

99.647

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

37.213

100

0.4
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.076

99.295

0.388
  pilF Thermus thermophilus HB27

37.655

99.295

0.374