Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HWV03_RS01335 Genome accession   NZ_CP056120
Coordinates   285718..287421 (-) Length   567 a.a.
NCBI ID   WP_211678154.1    Uniprot ID   -
Organism   Moritella sp. 36     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 280718..292421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV03_RS01310 (HWV03_01310) mutT 282125..282517 (+) 393 WP_211679944.1 8-oxo-dGTP diphosphatase MutT -
  HWV03_RS01315 (HWV03_01315) yacG 282540..282731 (-) 192 WP_211675603.1 DNA gyrase inhibitor YacG -
  HWV03_RS01320 (HWV03_01320) coaE 282758..283363 (-) 606 WP_211678151.1 dephospho-CoA kinase -
  HWV03_RS01325 (HWV03_01325) pilD 283375..284262 (-) 888 WP_211678152.1 A24 family peptidase Machinery gene
  HWV03_RS01330 (HWV03_01330) pilC 284382..285632 (-) 1251 WP_211678153.1 type II secretion system F family protein Machinery gene
  HWV03_RS01335 (HWV03_01335) pilB 285718..287421 (-) 1704 WP_211678154.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HWV03_RS22680 (HWV03_01340) - 287431..287865 (-) 435 WP_211678155.1 pilin -
  HWV03_RS01345 (HWV03_01345) nadC 288255..289097 (-) 843 WP_211673739.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HWV03_RS01350 (HWV03_01350) - 289122..289628 (-) 507 WP_211678156.1 retropepsin-like aspartic protease -
  HWV03_RS01355 (HWV03_01355) ampD 289773..290345 (+) 573 WP_211678157.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HWV03_RS01360 (HWV03_01360) ampE 290347..291195 (+) 849 WP_211678158.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62782.02 Da        Isoelectric Point: 5.1193

>NTDB_id=458211 HWV03_RS01335 WP_211678154.1 285718..287421(-) (pilB) [Moritella sp. 36]
MLHKHNAGGLAHSLVAHSYLVTEEIPQLLQSAKQEGKAFTTYLVDNNIIDSKILAQLLEREYGVPLLDLDTFKLEEIPDN
LLNGKLIEKHHALPIYVQGQTLYLAMSDPTNVTALEEFSFSFSLHTEVLLVDEVQLQKALEIVLESDIDALGDLNDATID
ELEVDNNESRLEQENNSGADDAPIVKFVNKILLDAIKKGASDIHFEPYEFKYRVRFRIDGILHEMVSPPVNLAMRFSARL
KVMAQLDIAERRVPQDGRIKLKLSKNKSIDLRVSTLPTMWGEKVVMRILDSNQASLNIDILGYDDTQKALYLKALEKPQG
MILITGPTGSGKTVSLYSGLNILNTVERNISTAEDPIEINLTGINQVQINLKAGLNFPSALRSFLRQDPDVVMVGEIRDI
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVPAYNIGSSVSLIIAQRLGRRLCSECKQPEDIPAIELAKLGFSQ
QQINDGITSFKAIGCNQCTKGYKGRVGIYEVMPMSDKIARMILTGSNSLELSEQAQQEGMNTLRQSALKKVIDGVTSLLE
VERITNH

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=458211 HWV03_RS01335 WP_211678154.1 285718..287421(-) (pilB) [Moritella sp. 36]
ATGCTACACAAACATAACGCTGGCGGCCTGGCTCACAGCCTGGTCGCACATTCATACCTTGTAACAGAAGAAATTCCCCA
ACTTCTGCAAAGCGCCAAACAGGAAGGTAAAGCCTTCACGACCTATCTCGTCGACAATAACATCATCGACAGTAAAATCT
TAGCGCAATTACTAGAGCGTGAATACGGCGTTCCACTACTCGATTTAGATACCTTTAAACTGGAAGAAATTCCCGATAAC
CTGCTTAATGGAAAATTAATCGAGAAACACCATGCATTGCCTATTTACGTGCAGGGACAAACATTATATCTGGCGATGTC
AGACCCGACCAATGTCACCGCATTAGAAGAGTTTTCCTTTAGCTTCAGTTTGCATACCGAAGTACTTTTGGTTGATGAAG
TACAATTACAAAAAGCGTTAGAGATTGTACTCGAAAGTGATATTGATGCCCTTGGTGATCTGAATGACGCCACCATTGAT
GAGTTAGAAGTTGATAACAACGAGTCACGCCTGGAGCAAGAAAATAACAGCGGTGCTGACGACGCCCCGATTGTTAAATT
CGTCAATAAGATCTTACTGGATGCGATTAAAAAAGGCGCATCCGATATTCACTTCGAGCCCTATGAATTCAAATACCGGG
TACGTTTTCGTATCGACGGTATCTTACATGAGATGGTATCGCCACCCGTAAACTTAGCGATGCGTTTTTCTGCGCGCTTA
AAAGTCATGGCGCAATTAGATATTGCCGAGCGTAGAGTGCCACAAGATGGCCGAATCAAATTAAAATTATCCAAAAACAA
GTCCATTGATCTACGTGTTAGCACCCTGCCCACCATGTGGGGTGAAAAAGTGGTGATGCGGATCCTAGATTCTAACCAAG
CCAGTCTCAACATTGATATTCTCGGTTATGACGATACACAAAAAGCCTTGTACCTTAAAGCGTTAGAAAAACCGCAGGGC
ATGATCTTGATCACAGGCCCAACAGGCAGTGGTAAAACGGTGTCTTTATACAGTGGCCTCAATATCCTCAACACCGTGGA
ACGCAACATATCCACAGCCGAAGATCCTATCGAGATCAACCTCACAGGTATCAATCAAGTCCAGATCAACCTGAAAGCAG
GACTGAACTTCCCCAGTGCATTGCGATCATTCTTACGCCAAGACCCGGATGTGGTCATGGTTGGTGAGATCCGCGATATA
GAAACAGCCGAGATTTCGATCAAAGCAGCGCAAACCGGTCACTTAGTATTATCGACTCTGCATACCAACTCTGCAGCAGA
AACCCTTACTCGTTTATCCAATATGGGTGTCCCTGCTTATAATATAGGTTCATCAGTCAGCTTGATCATCGCCCAACGGC
TCGGCCGTCGCCTGTGCAGTGAGTGTAAGCAGCCCGAAGACATACCCGCTATCGAATTAGCCAAGCTCGGTTTTAGCCAA
CAGCAAATCAATGATGGGATCACTTCATTTAAAGCCATAGGTTGTAACCAATGTACTAAAGGTTATAAAGGACGCGTAGG
GATCTACGAAGTCATGCCGATGTCAGATAAGATCGCACGCATGATCTTAACCGGCAGCAACTCGCTTGAACTCAGCGAAC
AAGCGCAGCAAGAAGGCATGAATACATTACGCCAATCCGCCTTGAAAAAAGTGATCGACGGCGTGACCAGTTTACTTGAG
GTTGAGCGTATCACTAATCATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.203

99.118

0.527
  pilB Legionella pneumophila strain ERS1305867

52.12

99.824

0.52
  pilB Acinetobacter baylyi ADP1

52.415

98.589

0.517
  pilB Vibrio cholerae strain A1552

52.632

93.827

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

51.756

95.414

0.494
  pilB Vibrio campbellii strain DS40M4

51.197

95.767

0.49
  pilF Neisseria gonorrhoeae MS11

46.726

99.647

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

36.498

100

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.076

99.295

0.388
  pilF Thermus thermophilus HB27

37.655

99.295

0.374