Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HV561_RS06945 Genome accession   NZ_CP055302
Coordinates   1343818..1345218 (+) Length   466 a.a.
NCBI ID   WP_176808294.1    Uniprot ID   -
Organism   Mannheimia pernigra strain 16CN0041     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1338818..1350218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV561_RS06915 (HV561_06915) - 1338930..1339694 (-) 765 WP_176808291.1 TSUP family transporter -
  HV561_RS06920 (HV561_06920) proB 1339694..1340785 (-) 1092 WP_159629587.1 glutamate 5-kinase -
  HV561_RS06925 (HV561_06925) - 1340899..1341393 (+) 495 WP_159629590.1 dihydrofolate reductase -
  HV561_RS06930 (HV561_06930) - 1341521..1341754 (+) 234 WP_159629593.1 YgjV family protein -
  HV561_RS06935 (HV561_06935) radA 1341793..1343172 (-) 1380 WP_176808292.1 DNA repair protein RadA Machinery gene
  HV561_RS06940 (HV561_06940) pilA 1343353..1343796 (+) 444 WP_176808293.1 prepilin peptidase-dependent pilin Machinery gene
  HV561_RS06945 (HV561_06945) pilB 1343818..1345218 (+) 1401 WP_176808294.1 GspE/PulE family protein Machinery gene
  HV561_RS06950 (HV561_06950) pilC 1345202..1346395 (+) 1194 WP_176808295.1 type II secretion system F family protein Machinery gene
  HV561_RS06955 (HV561_06955) - 1346398..1347054 (+) 657 WP_176808296.1 prepilin peptidase -
  HV561_RS11440 (HV561_06960) coaE 1347086..1347722 (+) 637 Protein_1331 dephospho-CoA kinase -
  HV561_RS11445 yacG 1347704..1347889 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  HV561_RS06970 (HV561_06965) holA 1347890..1348912 (-) 1023 WP_176808297.1 DNA polymerase III subunit delta -
  HV561_RS06975 (HV561_06970) lptE 1348953..1349441 (-) 489 WP_176808298.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 52729.49 Da        Isoelectric Point: 5.9563

>NTDB_id=457483 HV561_RS06945 WP_176808294.1 1343818..1345218(+) (pilB) [Mannheimia pernigra strain 16CN0041]
MAYSVTDLNSQQIIEISAEQWAKNGNEKQILLRYLAVPLRETESQLWLAIDDMQNLSACEIFAFIHHKQIEPVLISSDEL
KFLLNALSPEQSRLQSEIYEDNAAYSLSNAEQVDRNDPIIQILDNLFKFCLKNNASDIHLEPQKERLLIRLRIDGVLHLY
KTFPQNVSNRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRIQKNKPINLDFLELGFTP
SQKEKLLNALNQPQGLILVTGPTGSGKSITLYSALSHLNSNEKHILTAEDPIEIEIEGVIQTQVNRGIHLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDSPSAIERLLQLGIKEYELKNTLLLVVAQRLLRRLCTTCGGI
GCTHCYQGYKGRIGIYQLLSRTAKNFEKTTACLDFETLAESAKEKLVEGKTDQNEILRVLGNGENL

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=457483 HV561_RS06945 WP_176808294.1 1343818..1345218(+) (pilB) [Mannheimia pernigra strain 16CN0041]
ATGGCGTATTCAGTTACCGATCTTAACAGCCAGCAAATCATTGAAATTTCTGCCGAACAATGGGCAAAAAACGGCAATGA
AAAGCAGATTTTGTTGCGATATTTAGCCGTTCCGTTGCGAGAAACTGAATCACAATTATGGTTAGCGATTGATGATATGC
AAAACTTGAGTGCGTGTGAAATCTTTGCTTTTATTCATCATAAGCAGATTGAGCCTGTTTTGATTTCCTCTGATGAGTTG
AAATTTTTACTTAATGCCCTTTCACCCGAGCAGAGTCGCTTGCAGAGTGAAATTTATGAGGATAATGCCGCTTATTCGTT
ATCAAATGCAGAGCAGGTAGATAGAAACGATCCGATTATTCAAATTTTAGATAATCTCTTTAAATTTTGCTTAAAAAATA
ATGCCTCAGACATCCATTTGGAGCCTCAGAAAGAGAGGTTACTCATTCGGCTAAGAATTGATGGAGTTTTACATCTTTAT
AAAACGTTTCCTCAGAATGTATCAAACCGACTGATTTCTCGAATTAAATTACTAGCGAAACTAGATATTAGTGAAACCCG
TTTGCCTCAAGATGGGCAATTTCATTTTAAAACGATTTTAGCAGAAACCTTAGATTTCCGCGTTTCAACTTTGCCTACGC
ATTTTGGTGAAAAAGTGGTACTTCGGATTCAAAAAAATAAGCCAATTAATTTGGACTTTTTAGAGTTAGGATTTACCCCA
AGCCAGAAAGAAAAATTGCTCAATGCACTGAATCAACCGCAAGGCTTGATTCTAGTAACAGGGCCGACAGGTAGTGGTAA
AAGTATCACGCTTTATAGTGCATTAAGTCATTTAAATAGCAATGAAAAACATATTTTAACTGCGGAAGATCCAATTGAAA
TTGAGATAGAAGGTGTTATTCAAACTCAGGTAAATCGTGGTATTCATCTTGATTTTAGCCAACTACTGAGAACCTTCCTT
CGACAAGATCCTGACATCATTATGCTAGGTGAGATCCGTGATGAGGAGAGTGCAGAAATTGCCTTAAGAGCGGCACAAAC
TGGGCATTTGGTTCTTTCGACATTACATACTAATGATTCACCCTCTGCCATAGAGCGATTATTACAACTAGGCATTAAGG
AATATGAACTAAAAAATACATTATTACTCGTCGTTGCTCAACGTCTATTAAGGCGGCTTTGTACAACGTGCGGAGGAATT
GGATGTACGCATTGCTATCAAGGCTACAAAGGTAGAATTGGGATTTATCAACTCTTGAGCAGAACTGCAAAAAATTTTGA
AAAAACGACCGCTTGTTTAGATTTTGAAACCTTAGCTGAGAGTGCAAAAGAAAAATTAGTGGAAGGAAAAACAGATCAGA
ATGAAATTTTGAGGGTATTAGGAAATGGCGAAAATCTATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(131-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.217

98.712

0.693
  pilB Haemophilus influenzae 86-028NP

56.608

97.425

0.552
  pilB Haemophilus influenzae Rd KW20

56.388

97.425

0.549
  pilB Vibrio campbellii strain DS40M4

40.871

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.416
  pilB Vibrio cholerae strain A1552

39.627

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.809

100

0.406
  pilB Acinetobacter baylyi ADP1

37.5

100

0.393
  pilB Acinetobacter baumannii D1279779

36.605

100

0.384
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.206

89.485

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.076

100

0.376