Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK62_RS02965 Genome accession   NZ_CP055277
Coordinates   648814..650526 (-) Length   570 a.a.
NCBI ID   WP_176537051.1    Uniprot ID   A0A7H8SI53
Organism   Acinetobacter sp. NEB 394     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 643814..655526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK62_RS02935 (HUK62_02935) - 643911..644234 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  HUK62_RS02940 (HUK62_02940) rlmB 644377..645126 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  HUK62_RS02945 (HUK62_02945) - 645159..646076 (+) 918 WP_176537048.1 DMT family transporter -
  HUK62_RS02950 (HUK62_02950) coaE 646091..646690 (-) 600 WP_062035173.1 dephospho-CoA kinase -
  HUK62_RS02955 (HUK62_02955) pilD 646697..647557 (-) 861 WP_176537049.1 A24 family peptidase Machinery gene
  HUK62_RS02960 (HUK62_02960) pilC 647557..648783 (-) 1227 WP_176537050.1 type II secretion system F family protein Machinery gene
  HUK62_RS02965 (HUK62_02965) pilB 648814..650526 (-) 1713 WP_176537051.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK62_RS02970 (HUK62_02970) tpiA 650806..651597 (+) 792 WP_176537052.1 triose-phosphate isomerase -
  HUK62_RS02975 (HUK62_02975) secG 651611..651940 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  HUK62_RS03005 (HUK62_03005) rimP 652891..653415 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  HUK62_RS03010 (HUK62_03010) nusA 653455..654939 (+) 1485 WP_005400937.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63592.94 Da        Isoelectric Point: 5.0712

>NTDB_id=457370 HUK62_RS02965 WP_176537051.1 648814..650526(-) (pilB) [Acinetobacter sp. NEB 394]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKNLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=457370 HUK62_RS02965 WP_176537051.1 648814..650526(-) (pilB) [Acinetobacter sp. NEB 394]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTGGAATTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTATTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCTACCAGCAATCCAACCAACATGGATGCGATGGACGCAATTCGCTTCAATTCTAAAATGAATATTGAAGCGATTATTG
TTGAACATCCTCAACTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAAACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGC
GACCTTGAAACAGCTGAAATCGCAATTAAAGCTGCGCAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGTAATATGGGGGTTCCCTCTTTTAACATTGCGACCTCGGTCAATTTAGTGATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGACATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTTATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCTGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8SI53

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.602

100

0.367