Detailed information NTG0457368

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HUK62_RS02955
• Genome accession: NZ_CP055277
• Coordinates: 646697..647557 (-)
• Length: 286 a.a.
• NCBI ID: WP_176537049.1
• Uniprot ID: A0A7H8SEE5
• Organism: Acinetobacter sp. NEB 394
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 641697..652557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUK62_RS02925 (HUK62_02925)	recN	641858..643519 (-)	1662	WP_176537046.1	DNA repair protein RecN	-
HUK62_RS02930 (HUK62_02930)	-	643704..643844 (+)	141	WP_176537047.1	hypothetical protein	-
HUK62_RS02935 (HUK62_02935)	-	643911..644234 (+)	324	WP_004692718.1	pyrimidine/purine nucleoside phosphorylase	-
HUK62_RS02940 (HUK62_02940)	rlmB	644377..645126 (+)	750	WP_004692717.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
HUK62_RS02945 (HUK62_02945)	-	645159..646076 (+)	918	WP_176537048.1	DMT family transporter	-
HUK62_RS02950 (HUK62_02950)	coaE	646091..646690 (-)	600	WP_062035173.1	dephospho-CoA kinase	-
HUK62_RS02955 (HUK62_02955)	pilD	646697..647557 (-)	861	WP_176537049.1	A24 family peptidase	Machinery gene
HUK62_RS02960 (HUK62_02960)	pilC	647557..648783 (-)	1227	WP_176537050.1	type II secretion system F family protein	Machinery gene
HUK62_RS02965 (HUK62_02965)	pilB	648814..650526 (-)	1713	WP_176537051.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HUK62_RS02970 (HUK62_02970)	tpiA	650806..651597 (+)	792	WP_176537052.1	triose-phosphate isomerase	-
HUK62_RS02975 (HUK62_02975)	secG	651611..651940 (+)	330	WP_004692710.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31970.38 Da
• Isoelectric Point: 8.0620

>NTDB_id=457368 HUK62_RS02955 WP_176537049.1 646697..647557(-) (pilD) [Acinetobacter sp. NEB 394]
MQEIISYLINTPMALYFAVGVFSLCIGSFLNVVIYRTPKMMEQEWRTDCQLLLHPEQPIIDETKLTLSKPASTCPKCKSA
IHWYQNIPVMSWFLLRGKCGTCHNPISIRYPAIELLTAICALVVVAVFGPTIQMLFGLVLTYILIALTFIDFDTQLLPDR
YTLTLGGLGLGINSYAIYTTPTAAIWGYIIGFLCLWIVYYLFKLITGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSF
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKIYLGN

Nucleotide

• Length: 861 bp

>NTDB_id=457368 HUK62_RS02955 WP_176537049.1 646697..647557(-) (pilD) [Acinetobacter sp. NEB 394]
ATGCAAGAAATAATTTCCTATCTTATCAATACTCCAATGGCGCTTTATTTCGCCGTTGGAGTTTTTAGTTTGTGCATCGG
TAGTTTTCTGAATGTGGTGATCTATCGCACTCCAAAAATGATGGAACAAGAATGGCGCACCGACTGCCAACTTCTGCTTC
ATCCTGAGCAGCCAATTATTGATGAAACGAAGCTCACATTGAGTAAGCCTGCCTCAACCTGTCCAAAATGTAAAAGTGCC
ATACACTGGTATCAAAATATCCCTGTGATGAGTTGGTTCCTGCTACGAGGAAAGTGTGGCACTTGCCATAACCCGATCAG
CATTCGCTACCCTGCGATTGAACTCCTGACCGCCATTTGTGCTTTGGTCGTGGTTGCAGTATTTGGGCCAACAATCCAAA
TGCTGTTCGGTCTCGTGCTCACTTATATTTTAATCGCCTTAACATTTATTGATTTTGACACTCAGCTTTTACCTGATCGT
TATACCCTCACCCTTGGAGGTCTAGGTTTAGGTATCAATAGTTATGCGATTTACACCACACCAACAGCGGCCATTTGGGG
TTACATCATTGGCTTTTTATGCTTATGGATCGTGTACTATCTGTTTAAGCTCATCACTGGCAAGGAAGGTATGGGTTATG
GCGACTTTAAGTTACTTGCTGCTTTAGGCGCATGGATGGGCCCTTTGCTGCTGCCGCTGATTGTGCTACTTTCCTCTTTC
GTAGGTGCCATTATCGGCATCATCCTGCTCAAAGTGCGCAAAGAAAATCAGCCTTTTGCTTTTGGTCCGTATATTGCCAT
CGCAGGATGGATTGCCTTTTTGTGGGGTGAGCAAATTATGAAAATATACTTAGGCAATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A7H8SEE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	79.298	99.65	0.79
pilD	Acinetobacter nosocomialis M2	79.298	99.65	0.79
pilD	Vibrio cholerae strain A1552	54.478	93.706	0.51
pilD	Vibrio campbellii strain DS40M4	51.301	94.056	0.483
pilD	Neisseria gonorrhoeae MS11	45.788	95.455	0.437