Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HUT20_RS11915 Genome accession   NZ_CP054874
Coordinates   2579971..2581188 (-) Length   405 a.a.
NCBI ID   WP_015637067.1    Uniprot ID   A0A2C9ETK4
Organism   Pseudomonas protegens strain PGNL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2574971..2586188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUT20_RS11875 (HUT20_11875) - 2575688..2575861 (-) 174 WP_015637073.1 DUF3094 family protein -
  HUT20_RS11880 (HUT20_11880) - 2576051..2576680 (-) 630 WP_011063528.1 DUF1780 domain-containing protein -
  HUT20_RS11885 (HUT20_11885) - 2576824..2577291 (+) 468 WP_026019989.1 hypothetical protein -
  HUT20_RS11890 (HUT20_11890) - 2577288..2577977 (+) 690 WP_015637070.1 energy-coupling factor ABC transporter permease -
  HUT20_RS11895 (HUT20_11895) - 2578041..2578256 (+) 216 WP_011063525.1 hypothetical protein -
  HUT20_RS11900 (HUT20_11900) yacG 2578276..2578479 (-) 204 WP_015637069.1 DNA gyrase inhibitor YacG -
  HUT20_RS11905 (HUT20_11905) coaE 2578476..2579099 (-) 624 WP_011063523.1 dephospho-CoA kinase -
  HUT20_RS11910 (HUT20_11910) pilD 2579096..2579968 (-) 873 WP_015637068.1 prepilin peptidase Machinery gene
  HUT20_RS11915 (HUT20_11915) pilC 2579971..2581188 (-) 1218 WP_015637067.1 type II secretion system F family protein Machinery gene
  HUT20_RS11920 (HUT20_11920) - 2581518..2581934 (+) 417 WP_011063520.1 pilin -
  HUT20_RS11925 (HUT20_11925) - 2582163..2582519 (+) 357 WP_011063519.1 BON domain-containing protein -
  HUT20_RS11930 (HUT20_11930) - 2582730..2585648 (-) 2919 WP_041752592.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43815.46 Da        Isoelectric Point: 10.5006

>NTDB_id=455168 HUT20_RS11915 WP_015637067.1 2579971..2581188(-) (pilC) [Pseudomonas protegens strain PGNL1]
MAVKAPATSVYLWHGTDSQGRPLTGELSAATPALIRAQLRRQGITPTRVRRKPKGWLRLHQRVGARDIALLTRQLATLMQ
AGVPLLQSFDIIAESFEHPQMRQLLASLKRDIEAGSSLATALRRQPRHFDPLYCNLVDAGEQAGALDTLLERVATYKEKS
ENLKARLKKAMVYPLAVIGVAVVVSSILLLKVVPQFQSLFAGFNAELPLLTQWVIALSHFIQQQGALLLAAGVLGGLAAR
LAYQRSPSLRDRLDVGLLHMPLAGPLLHKSAVARYARTLATTFAAGVPLVQALDSVAGASGNPVFRQAVQRIRQQVAAGM
QLNFAMRASGVFPTMAIQMTAIGEESGTLDHLLEKVAIHYEVEVDNLVDSLTSLMEPLIMVVLGGIVGALVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=455168 HUT20_RS11915 WP_015637067.1 2579971..2581188(-) (pilC) [Pseudomonas protegens strain PGNL1]
ATGGCGGTAAAAGCACCCGCGACCAGTGTCTATCTCTGGCACGGCACCGACAGCCAGGGCCGCCCCCTCACCGGAGAACT
CAGCGCCGCGACCCCGGCCCTGATCAGGGCGCAATTGCGCAGACAGGGCATCACCCCAACCCGAGTCCGACGCAAACCCA
AGGGCTGGCTGCGCCTGCATCAGCGAGTAGGGGCACGGGACATTGCCCTGCTCACCCGGCAGTTGGCCACGCTGATGCAG
GCCGGGGTGCCGCTGCTGCAATCCTTCGACATCATTGCCGAAAGTTTCGAGCATCCGCAGATGCGTCAACTGCTGGCCAG
CCTCAAGCGCGACATCGAGGCCGGCTCCAGCCTGGCCACGGCATTGCGCAGACAGCCGCGCCACTTCGACCCGCTGTACT
GCAACCTGGTGGACGCCGGCGAGCAGGCCGGCGCCCTGGATACCCTGCTGGAAAGAGTCGCCACCTATAAGGAAAAGAGC
GAGAACCTGAAGGCGCGGCTGAAGAAAGCCATGGTCTATCCCTTGGCGGTGATCGGCGTGGCGGTCGTGGTCAGCAGCAT
CCTGCTGCTCAAGGTGGTCCCCCAGTTCCAGAGCTTGTTCGCCGGTTTCAATGCCGAGCTGCCACTGCTCACCCAGTGGG
TCATCGCTCTGTCACATTTCATCCAGCAGCAGGGGGCGCTGCTGCTGGCTGCCGGTGTGCTGGGCGGGCTCGCAGCGCGC
CTGGCCTATCAGCGCTCCCCGAGCTTGCGTGACCGCCTGGATGTCGGCCTTTTGCACATGCCCCTGGCCGGGCCACTGCT
GCATAAATCCGCGGTGGCCCGCTACGCACGGACCCTGGCCACCACCTTCGCCGCCGGCGTTCCCCTGGTACAGGCCCTGG
ATTCAGTGGCCGGAGCCAGCGGCAACCCTGTCTTCAGGCAAGCGGTGCAACGGATCCGCCAGCAGGTCGCCGCCGGCATG
CAATTGAACTTTGCCATGCGCGCCAGCGGCGTCTTCCCGACCATGGCGATCCAGATGACCGCCATCGGCGAAGAGTCCGG
AACCCTGGATCATCTGCTGGAAAAAGTCGCGATCCATTACGAGGTCGAGGTGGACAACCTGGTGGACAGCCTGACCAGCC
TCATGGAGCCGTTGATCATGGTGGTCCTGGGGGGTATCGTCGGCGCCCTGGTGGTTGCCATGTACCTGCCTATCTTCCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C9ETK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.765

100

0.588
  pilC Acinetobacter baylyi ADP1

53.056

100

0.536
  pilC Acinetobacter baumannii D1279779

53.186

100

0.536
  pilC Legionella pneumophila strain ERS1305867

47.059

100

0.474
  pilC Vibrio cholerae strain A1552

43.401

97.284

0.422
  pilG Neisseria gonorrhoeae MS11

42.289

99.259

0.42
  pilG Neisseria meningitidis 44/76-A

42.394

99.012

0.42
  pilC Vibrio campbellii strain DS40M4

41.542

99.259

0.412
  pilC Thermus thermophilus HB27

38

98.765

0.375