Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUI97_RS18465 Genome accession   NZ_CP054855
Coordinates   4047062..4048768 (+) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain 1708-29120     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4042062..4053768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUI97_RS18440 (HUI97_18440) pdhR 4043204..4043968 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HUI97_RS18445 (HUI97_18445) ampD 4044247..4044822 (-) 576 WP_206900638.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUI97_RS18450 (HUI97_18450) - 4044953..4045423 (+) 471 WP_206900639.1 retropepsin-like aspartic protease family protein -
  HUI97_RS18455 (HUI97_18455) nadC 4045427..4046290 (+) 864 WP_206900640.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUI97_RS18460 (HUI97_18460) - 4046606..4047058 (+) 453 WP_108541638.1 pilin -
  HUI97_RS18465 (HUI97_18465) pilB 4047062..4048768 (+) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HUI97_RS18470 (HUI97_18470) pilC 4049007..4050248 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  HUI97_RS18475 (HUI97_18475) pilD 4050310..4051182 (+) 873 WP_206900641.1 prepilin peptidase Machinery gene
  HUI97_RS18480 (HUI97_18480) coaE 4051200..4051808 (+) 609 WP_206900642.1 dephospho-CoA kinase -
  HUI97_RS18485 (HUI97_18485) zapD 4051853..4052575 (+) 723 WP_041210678.1 cell division protein ZapD -
  HUI97_RS18490 (HUI97_18490) yacG 4052585..4052779 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  HUI97_RS18495 (HUI97_18495) mutT 4052852..4053256 (-) 405 WP_206900643.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=454999 HUI97_RS18465 WP_005336134.1 4047062..4048768(+) (pilB) [Aeromonas veronii strain 1708-29120]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=454999 HUI97_RS18465 WP_005336134.1 4047062..4048768(+) (pilB) [Aeromonas veronii strain 1708-29120]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCTTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAATGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGCGCCTCAGACCTGCACTTCGAGCCTTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATAGATGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCCACCGCAGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAAATACGGGAT
CTAGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCATCTGGTGCTCTCCACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCAGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAATATCGCCAAATTGATCATGCAGGGCGCCAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAAGAGGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377