Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUI95_RS19605 Genome accession   NZ_CP054854
Coordinates   4258622..4260337 (+) Length   571 a.a.
NCBI ID   WP_206867229.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 1706-28330     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4253622..4265337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUI95_RS19580 (HUI95_19555) pdhR 4254801..4255565 (-) 765 WP_005305630.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HUI95_RS19585 (HUI95_19560) ampD 4255841..4256413 (-) 573 WP_206865932.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUI95_RS19590 (HUI95_19565) - 4256537..4257007 (+) 471 WP_017779055.1 retropepsin-like aspartic protease -
  HUI95_RS19595 (HUI95_19570) nadC 4257012..4257869 (+) 858 WP_094697628.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUI95_RS19600 (HUI95_19575) - 4258191..4258631 (+) 441 WP_206865933.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HUI95_RS19605 (HUI95_19580) pilB 4258622..4260337 (+) 1716 WP_206867229.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HUI95_RS19610 (HUI95_19585) pilC 4260453..4261694 (+) 1242 WP_010635960.1 type II secretion system F family protein Machinery gene
  HUI95_RS19615 (HUI95_19590) pilD 4261717..4262589 (+) 873 WP_095591547.1 A24 family peptidase Machinery gene
  HUI95_RS19620 (HUI95_19595) coaE 4262609..4263223 (+) 615 WP_005305656.1 dephospho-CoA kinase -
  HUI95_RS19625 (HUI95_19600) zapD 4263261..4263983 (+) 723 WP_010635957.1 cell division protein ZapD -
  HUI95_RS19630 (HUI95_19605) yacG 4263993..4264187 (+) 195 WP_017782442.1 DNA gyrase inhibitor YacG -
  HUI95_RS19635 (HUI95_19610) mutT 4264248..4264661 (-) 414 WP_005305665.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62870.37 Da        Isoelectric Point: 5.4321

>NTDB_id=454969 HUI95_RS19605 WP_206867229.1 4258622..4260337(+) (pilB) [Aeromonas dhakensis strain 1706-28330]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAISKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKMSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEILLMSENIAKLIMQGANSMQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=454969 HUI95_RS19605 WP_206867229.1 4258622..4260337(+) (pilB) [Aeromonas dhakensis strain 1706-28330]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAGGCCAAGGCACAGCGCAAGCCCTTTGTGACCTTTCTGATCGAGAACGACATCCTCGACAGCA
AGGCGCTAGCCGACTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCTGCCTTCGACCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCCGACCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGTTTCGGGCTGCACACCGAGGCCCTGCTGGTCG
AGGAGAACAAGCTGACCGCTGCCATCAGCAAGCTGATGGAGAACGATCAGGACGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCCGAGCTGGAGGTGTCTGACGAGGGCTCACGACTCGACGAGAGCGTCAACACGGCGGACGATGACGCCCC
CATCGTCAAATACATAAACAAGATCATGATGGATGCCATCAAGCGCGGTGCATCCGACCTCCACTTCGAGCCCTATGAGA
CCAAGTACCGTATTCGCTTTCGGATAGACGGGATCCTGCATGAGGTAGCAACGCCGCCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCGCGCCTGGACATCGCCGAACGGCGGCTGCCCCAGGATGGCCGCATCAAATTGAAAAT
GTCGCGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTCG
ACTCTTCCGCTGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAGGCACAATATCTGCGCGCGCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACAGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCT
CAACACCACAGAAGTGAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCTGGGGTCAATCAGGTACAGGTCA
ACCCCAAGGCTGGGCTGACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACGTAGTGATGGTCGGGGAA
ATACGGGATCTTGAAACCGCCGAGATCGCCATCAAGGCGGCACAAACCGGTCACCTGGTGCTCTCTACATTGCACACCAA
CTCGGCGGCCGAGACGCTGACCCGGATGATGAACATGGGAGTCCCCGCCTTCAACATCGCCTCCTCGGTTACCCTGATCA
TGGCTCAACGATTGGCACGCAAGCTGTGCGACCACTGCAAGGCCCCCGAAGTGGTGCCGGAAGCTGAACTGCTGGAGTTG
GGGTTTACCCAACAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAGCCGGTTGGTTGCAAGGAGTGTTCGGGCGGCTACAA
GGGGCGGGTCGGCATCTACGAGATCCTCTTGATGTCAGAAAATATTGCAAAATTGATCATGCAGGGAGCCAATTCCATGC
AGATTGCGGCCATTGCCCAGAAGGAGGGTATGCGGACCTTGCGGACCTCCGGTCTTGAGAAGGCCCGACTGGGGGTGACC
AGCCTGGCCGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.148

100

0.553
  pilB Acinetobacter baumannii D1279779

54.53

100

0.548
  pilB Legionella pneumophila strain ERS1305867

54.417

99.124

0.539
  pilB Vibrio cholerae strain A1552

53.805

98.949

0.532
  pilB Vibrio campbellii strain DS40M4

51.59

99.124

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

50.89

98.424

0.501
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

96.848

0.375
  pilF Thermus thermophilus HB27

40.53

92.469

0.375