Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HUK70_RS25700 Genome accession   NZ_CP054789
Coordinates   5402544..5404244 (+) Length   566 a.a.
NCBI ID   WP_010793793.1    Uniprot ID   A0A367MDN5
Organism   Pseudomonas aeruginosa strain SE5381     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5397544..5409244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK70_RS25675 (HUK70_25520) ampD 5397577..5398143 (-) 567 WP_003116245.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HUK70_RS25680 (HUK70_25525) - 5398294..5400549 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  HUK70_RS25685 (HUK70_25530) nadC 5400835..5401683 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUK70_RS25695 (HUK70_25540) pilA 5401860..5402312 (-) 453 WP_024928732.1 pilin Machinery gene
  HUK70_RS25700 (HUK70_25545) pilB 5402544..5404244 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK70_RS25705 (HUK70_25550) pilC 5404248..5405468 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  HUK70_RS25710 (HUK70_25555) pilD 5405472..5406344 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HUK70_RS25715 (HUK70_25560) coaE 5406341..5406952 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  HUK70_RS25720 (HUK70_25565) yacG 5406949..5407149 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HUK70_RS25725 (HUK70_25570) - 5407186..5407395 (-) 210 WP_003094660.1 hypothetical protein -
  HUK70_RS25730 (HUK70_25575) - 5407501..5408190 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  HUK70_RS25735 (HUK70_25580) - 5408187..5408657 (-) 471 WP_010793791.1 hypothetical protein -
  HUK70_RS25740 (HUK70_25585) - 5408654..5409079 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62354.24 Da        Isoelectric Point: 5.8580

>NTDB_id=454568 HUK70_RS25700 WP_010793793.1 5402544..5404244(+) (pilB) [Pseudomonas aeruginosa strain SE5381]
MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=454568 HUK70_RS25700 WP_010793793.1 5402544..5404244(+) (pilB) [Pseudomonas aeruginosa strain SE5381]
ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A367MDN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.887

99.647

0.507
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.645

99.647

0.495
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366