Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HUK70_RS25710 Genome accession   NZ_CP054789
Coordinates   5405472..5406344 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5381     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5400472..5411344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK70_RS25685 (HUK70_25530) nadC 5400835..5401683 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HUK70_RS25695 (HUK70_25540) pilA 5401860..5402312 (-) 453 WP_024928732.1 pilin Machinery gene
  HUK70_RS25700 (HUK70_25545) pilB 5402544..5404244 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK70_RS25705 (HUK70_25550) pilC 5404248..5405468 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  HUK70_RS25710 (HUK70_25555) pilD 5405472..5406344 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HUK70_RS25715 (HUK70_25560) coaE 5406341..5406952 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  HUK70_RS25720 (HUK70_25565) yacG 5406949..5407149 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HUK70_RS25725 (HUK70_25570) - 5407186..5407395 (-) 210 WP_003094660.1 hypothetical protein -
  HUK70_RS25730 (HUK70_25575) - 5407501..5408190 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  HUK70_RS25735 (HUK70_25580) - 5408187..5408657 (-) 471 WP_010793791.1 hypothetical protein -
  HUK70_RS25740 (HUK70_25585) - 5408654..5409079 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  HUK70_RS25745 (HUK70_25590) - 5409213..5409842 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HUK70_RS25750 (HUK70_25595) - 5409839..5410288 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  HUK70_RS25755 (HUK70_25600) - 5410314..5410484 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=454570 HUK70_RS25710 WP_003125204.1 5405472..5406344(+) (pilD) [Pseudomonas aeruginosa strain SE5381]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=454570 HUK70_RS25710 WP_003125204.1 5405472..5406344(+) (pilD) [Pseudomonas aeruginosa strain SE5381]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATACTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTACCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472