Detailed information NTG0453882

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: FOB47_RS23055
• Genome accession: NZ_CP054626
• Coordinates: 2852012..2853310 (+)
• Length: 432 a.a.
• NCBI ID: WP_174782185.1
• Uniprot ID: -
• Organism: Cupriavidus gilardii strain FDAARGOS_639
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2847012..2858310

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOB47_RS23025 (FOB47_23025)	proB	2847235..2848359 (-)	1125	WP_053822554.1	glutamate 5-kinase	-
FOB47_RS23030 (FOB47_23030)	obgE	2848422..2849519 (-)	1098	WP_053822555.1	GTPase ObgE	-
FOB47_RS23035 (FOB47_23035)	rpmA	2849657..2849917 (-)	261	WP_053822556.1	50S ribosomal protein L27	-
FOB47_RS23040 (FOB47_23040)	rplU	2849981..2850292 (-)	312	WP_006576525.1	50S ribosomal protein L21	-
FOB47_RS23045 (FOB47_23045)	ispB	2850836..2851765 (+)	930	WP_053822557.1	octaprenyl diphosphate synthase	-
FOB47_RS23055 (FOB47_23055)	pilF	2852012..2853310 (+)	1299	WP_174782185.1	GspE/PulE family protein	Machinery gene
FOB47_RS23060 (FOB47_23060)	-	2853344..2854468 (+)	1125	WP_174782186.1	type II secretion system F family protein	-
FOB47_RS23065 (FOB47_23065)	-	2854470..2855411 (+)	942	WP_276512689.1	prepilin peptidase	-
FOB47_RS23070 (FOB47_23070)	coaE	2855424..2856056 (+)	633	WP_174782187.1	dephospho-CoA kinase	-
FOB47_RS23075 (FOB47_23075)	zapD	2856307..2857065 (+)	759	WP_006578256.1	cell division protein ZapD	-
FOB47_RS23080 (FOB47_23080)	-	2857072..2857260 (+)	189	WP_053822560.1	DNA gyrase inhibitor YacG	-
FOB47_RS23085 (FOB47_23085)	-	2857288..2857692 (-)	405	WP_053822561.1	NUDIX domain-containing protein	-

Sequence

Protein

• Length: 432 a.a.
• Molecular weight: 46683.86 Da
• Isoelectric Point: 7.9556

>NTDB_id=453882 FOB47_RS23055 WP_174782185.1 2852012..2853310(+) (pilF) [Cupriavidus gilardii strain FDAARGOS_639]
MIAVTSPSDAPSDAPIDAPIDAPIEYDPAMPSADDAPVVRFVHRLLAEACRRGASDLHFEPYEGFYRVRFRIDGQLHEVA
RPPLDIRDRIATRIKVLARLDIAEKRVPQDGRMRFVVPRRDGEATPDGAAGRAVDLRVSTLPTLFGEKIVLRMLAASSVR
LDMDSLGLEPTQLAVLLERLRRPHGMVLVTGPTGSGKTVSLYAFLNLLNQHERNISTAEDPAEIQLAGINQVNIHDKIGL
NFPTVLRALLRQDPDVIMVGEIRDAETADVAVKAAQTGHLVLSTLHTNDAPGTLTRLAQLGVAPFHLAASVLVITAQRLA
RMLCACKRPVPVPLSALREAGFADTLLDGDWQPCQPVGCAACRGSGYRGRCGIHQVMPVSDAMQNIILSRGSARALAQQA
RREGVLSLREAGLLKVRAGTTSLAEVLATTNP

Nucleotide

• Length: 1299 bp

>NTDB_id=453882 FOB47_RS23055 WP_174782185.1 2852012..2853310(+) (pilF) [Cupriavidus gilardii strain FDAARGOS_639]
ATGATCGCCGTGACCTCCCCGTCCGATGCGCCGTCCGATGCGCCGATCGATGCGCCGATCGATGCGCCGATCGAATACGA
CCCCGCCATGCCGTCGGCCGACGACGCGCCGGTCGTGCGCTTCGTCCACAGGCTGCTGGCCGAGGCCTGCCGGCGCGGCG
CCTCGGACCTGCACTTCGAGCCGTACGAAGGCTTCTATCGCGTGCGCTTTCGCATCGATGGGCAGCTGCACGAGGTCGCC
CGTCCGCCGCTGGACATTCGCGATCGCATCGCGACGCGGATCAAGGTGCTGGCGCGGCTGGACATCGCCGAGAAGCGTGT
ACCGCAGGATGGCCGCATGCGGTTCGTGGTGCCGCGGCGCGACGGCGAGGCGACGCCTGACGGGGCCGCCGGCCGCGCGG
TCGATCTGCGCGTATCGACGCTGCCGACGCTGTTCGGCGAAAAGATCGTGCTGCGCATGCTGGCCGCGTCCAGCGTGCGG
CTCGATATGGACAGCCTGGGCCTCGAGCCGACGCAGCTGGCCGTGCTGCTCGAGCGGCTGCGCCGTCCGCACGGCATGGT
GCTGGTCACCGGTCCGACCGGCAGCGGCAAGACCGTTTCGCTGTACGCCTTCCTGAATCTGCTGAACCAGCACGAGCGCA
ATATCTCGACGGCCGAGGACCCGGCCGAGATCCAGCTGGCCGGCATCAACCAGGTCAATATCCACGACAAGATCGGCCTG
AACTTCCCCACCGTGCTGCGCGCGTTGCTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATGCCGAAAC
CGCCGATGTCGCGGTCAAGGCGGCGCAGACCGGCCATCTGGTCCTGTCGACGCTGCACACCAACGATGCGCCGGGCACGC
TGACACGGCTCGCGCAGCTTGGCGTCGCGCCCTTTCACCTGGCCGCCAGCGTGCTGGTGATCACCGCGCAGCGGCTGGCC
CGCATGCTGTGCGCCTGCAAACGTCCGGTCCCGGTGCCGCTGTCCGCGCTGCGCGAGGCCGGTTTCGCGGACACGCTGCT
CGACGGCGACTGGCAGCCCTGCCAGCCGGTCGGCTGCGCCGCCTGCCGCGGCAGCGGTTATCGCGGGCGTTGCGGCATCC
ATCAGGTGATGCCGGTCTCCGACGCGATGCAGAACATCATCCTGTCGCGGGGCAGTGCCCGTGCGCTGGCCCAGCAGGCG
CGCCGCGAGGGCGTGCTATCGTTGCGCGAGGCGGGGCTGCTCAAGGTCAGGGCGGGCACGACCTCGCTGGCCGAGGTGCT
GGCGACGACCAATCCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (45-322)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Neisseria gonorrhoeae MS11	57.357	92.824	0.532
pilB	Acinetobacter baumannii D1279779	52.771	96.065	0.507
pilB	Acinetobacter baylyi ADP1	54.198	90.972	0.493
pilB	Vibrio cholerae strain A1552	52.736	93.056	0.491
pilB	Legionella pneumophila strain ERS1305867	50	94.444	0.472
pilB	Vibrio parahaemolyticus RIMD 2210633	51.01	91.667	0.468
pilB	Vibrio campbellii strain DS40M4	50.758	91.667	0.465
pilB/pilB1	Synechocystis sp. PCC 6803	42.584	96.759	0.412
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.68	93.287	0.398
pilF	Thermus thermophilus HB27	41.96	92.13	0.387
pilB	Glaesserella parasuis strain SC1401	39.234	96.759	0.38
pilB	Haemophilus influenzae 86-028NP	38.756	96.759	0.375
pilB	Haemophilus influenzae Rd KW20	38.517	96.759	0.373