Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   I5367_RS08525 Genome accession   NZ_CP065539
Coordinates   1689352..1691937 (+) Length   861 a.a.
NCBI ID   WP_041482253.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1684352..1696937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS08505 (I5367_08505) - 1685151..1686329 (+) 1179 WP_098081411.1 glycine C-acetyltransferase -
  I5367_RS08510 (I5367_08510) miaB 1686472..1688001 (+) 1530 WP_012117585.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  I5367_RS08515 (I5367_08515) ymcA 1688004..1688435 (+) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  I5367_RS08520 (I5367_08520) cotE 1688688..1689233 (+) 546 WP_003154111.1 outer spore coat protein CotE -
  I5367_RS08525 (I5367_08525) hexA 1689352..1691937 (+) 2586 WP_041482253.1 DNA mismatch repair protein MutS Machinery gene
  I5367_RS08530 (I5367_08530) hexB 1691953..1693827 (+) 1875 WP_012117587.1 DNA mismatch repair endonuclease MutL Machinery gene
  I5367_RS08535 (I5367_08535) - 1694221..1694430 (-) 210 WP_041482193.1 hypothetical protein -
  I5367_RS08540 (I5367_08540) - 1695395..1696072 (+) 678 WP_012117588.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97810.04 Da        Isoelectric Point: 5.3045

>NTDB_id=449994 I5367_RS08525 WP_041482253.1 1689352..1691937(+) (hexA) [Bacillus amyloliquefaciens strain DGL1]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRDGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVSAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAVLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAVSEKK
SAVREEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=449994 I5367_RS08525 WP_041482253.1 1689352..1691937(+) (hexA) [Bacillus amyloliquefaciens strain DGL1]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGATGGAAAAGGCATTCATGAATCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTCC
GTGACGGATACGGTCTCGCTTTATCGGACTTAACGACTGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCAGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCCACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACAATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCGATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGAGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGGGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATCAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAATTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAAGACGGACGGTATGAGCGGAAA
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGTACTGCGTGAAAAGGTGAAACAATTTATTCCGCGCTTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGACGCACTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTATATGAGAC
AGATCGCACTTCTATCGATCATGGCTCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTCCATGATCATATCGGTGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGTATCTGAGAAAAAG
TCCGCCGTCAGAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(598-785)

MutS domain II (MutS_II)

(125-239)

MutS domain I (MutS_I)

(5-116)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain III (MutS_III)

(258-547)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.191

99.535

0.48
  mutS Pseudomonas stutzeri strain ATCC 17587

38.549

100

0.395