Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G4D73_RS10910 Genome accession   NZ_CP053947
Coordinates   2346878..2348587 (-) Length   569 a.a.
NCBI ID   WP_213686756.1    Uniprot ID   -
Organism   Acinetobacter sp. WY4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2341878..2353587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4D73_RS10880 - 2341997..2342320 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  G4D73_RS10885 rlmB 2342455..2343204 (+) 750 WP_005101440.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G4D73_RS10890 - 2343211..2344134 (+) 924 WP_416232271.1 DMT family transporter -
  G4D73_RS10895 coaE 2344156..2344755 (-) 600 WP_213686753.1 dephospho-CoA kinase -
  G4D73_RS15630 pilD 2344757..2345617 (-) 861 WP_213686754.1 prepilin peptidase Machinery gene
  G4D73_RS10905 pilC 2345617..2346846 (-) 1230 WP_213686755.1 type II secretion system F family protein Machinery gene
  G4D73_RS10910 pilB 2346878..2348587 (-) 1710 WP_213686756.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G4D73_RS10915 tpiA 2348865..2349656 (+) 792 WP_213686757.1 triose-phosphate isomerase -
  G4D73_RS10920 secG 2349670..2349999 (+) 330 WP_148334344.1 preprotein translocase subunit SecG -
  G4D73_RS10950 rimP 2351066..2351590 (+) 525 WP_213686758.1 ribosome maturation factor RimP -
  G4D73_RS10955 nusA 2351625..2353109 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63485.68 Da        Isoelectric Point: 4.8339

>NTDB_id=449308 G4D73_RS10910 WP_213686756.1 2346878..2348587(-) (pilB) [Acinetobacter sp. WY4]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDESNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLATRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=449308 G4D73_RS10910 WP_213686756.1 2346878..2348587(-) (pilB) [Acinetobacter sp. WY4]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCGTTGGCGCATGCCAAACAAGAAAAAATCGATATTGTGGCTGAGTTAATTAACCAGCAGCAACTTTCTC
CAACCGTCATTGCTGAAACGATTTCAGTCGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCTCAAATT
CTTCGGGATGCGATTGATGAAAAGCTAATTACCAAGCATCGCATTCTGCCGATTTTTAAAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCGACCAATATCGAAGCGATTGATGCAGTCCGTTTTTCAACTAAACTCAATATTGAAACGATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGCACGTTTGAGTTTGATGAAGACTTTGAC
TTAGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGAAAGCAATAAAGGTGATGAAGCACCAATTGTCAA
ATATATAAATAAGTTACTCATCGATGCGATCCGTATGGGGGCTTCAGACTTACATTTTGAACCTTATGAGAAAATCTATC
GGGTACGTTACCGGGTCGATGGGGTATTACGCCAGATTGCAACACCACCACTACAGCTAGCAACTCGTTTATCTTCACGT
CTTAAAGTCATGTCGCAAATGGACATTTCAGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGCGAGAAGCTGGTACTGCGTATTCTGGATCCATCCA
GTGCGATGCTGGGGATTGATGCTCTAGGCTATGAACCGGAACAAAAAGAACTGTTTATGCAGGCGCTGGACAAACCGCAA
GGGATGCTGCTGATCACCGGCCCGACGGGTTCCGGTAAAACAGTTTCTCTTTACACCGGTCTGAATATCCTAAACCGTGA
AGATACCAATATCTCAACTGCGGAAGATCCGGTCGAGATTAACTTGCAAGGCATTAATCAGGTCAACGTCAATAATAAAG
TTGGCCTGACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGATATCGTGATGGTTGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCGGCGCAGACCGGTCATATGGTGATGTCGACGCTGCATACCAATAGTGCGCC
TGAGACTTTGACCCGTTTACGCAATATGGGGGTGCCTTCTTTCAATATTGCTACTTCAGTCAACCTGGTCATTGCACAAC
GGTTGGCACGTCGTTTGTGTTCACAATGCAAGAAACCTGCCGATATTCCGAAACAAAGCTTACTGGAGATGGGTTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGTCGTGAAGGCTATAAGGGTCG
TGTGGGTATTTATGAAGTCATGAAAGTAACACCCGAAATTTCCAGAATTATTATGGAGGACGGCAATGCACTTGAAATTG
CCGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAGTCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.702

100

0.808
  pilB Acinetobacter baylyi ADP1

76.842

100

0.77
  pilB Legionella pneumophila strain ERS1305867

51.767

99.473

0.515
  pilF Neisseria gonorrhoeae MS11

49.198

98.594

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

49.374

98.243

0.485
  pilB Vibrio campbellii strain DS40M4

48.925

98.067

0.48
  pilB Vibrio cholerae strain A1552

50.87

90.861

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

35.237

100

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.932

100

0.373
  pilF Thermus thermophilus HB27

37.213

99.649

0.371