Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ395_RS04955 Genome accession   NZ_CP053885
Coordinates   1032276..1033982 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 3019     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1027276..1038982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ395_RS04925 (HQ395_04925) mutT 1027951..1028364 (+) 414 WP_174892455.1 8-oxo-dGTP diphosphatase MutT -
  HQ395_RS04930 (HQ395_04930) yacG 1028425..1028619 (-) 195 WP_029302063.1 DNA gyrase inhibitor YacG -
  HQ395_RS04935 (HQ395_04935) zapD 1028629..1029351 (-) 723 WP_016352172.1 cell division protein ZapD -
  HQ395_RS04940 (HQ395_04940) coaE 1029389..1030003 (-) 615 WP_029302064.1 dephospho-CoA kinase -
  HQ395_RS04945 (HQ395_04945) pilD 1030024..1030896 (-) 873 WP_029302065.1 A24 family peptidase Machinery gene
  HQ395_RS04950 (HQ395_04950) pilC 1030919..1032160 (-) 1242 WP_029302066.1 type II secretion system F family protein Machinery gene
  HQ395_RS04955 (HQ395_04955) pilB 1032276..1033982 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HQ395_RS04960 (HQ395_04960) tapA 1033986..1034438 (-) 453 WP_041213796.1 type IVa pilus major pilin TapA -
  HQ395_RS04965 (HQ395_04965) nadC 1034761..1035618 (-) 858 WP_029302069.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HQ395_RS04970 (HQ395_04970) - 1035624..1036094 (-) 471 WP_029302070.1 retropepsin-like aspartic protease -
  HQ395_RS04975 (HQ395_04975) ampD 1036242..1036814 (+) 573 WP_029302071.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HQ395_RS04980 (HQ395_04980) pdhR 1037090..1037854 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=448435 HQ395_RS04955 WP_029302067.1 1032276..1033982(-) (pilB) [Aeromonas hydrophila strain 3019]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=448435 HQ395_RS04955 WP_029302067.1 1032276..1033982(-) (pilB) [Aeromonas hydrophila strain 3019]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAAGCATTGG
CGGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACATCACGTCCTACCCATCTATACCCAAGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGATGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCGCCCATCGTCAA
ATACATCAACAAAATCATGATGGACGCCATCAAGCGCGGAGCCTCCGACCTCCACTTCGAACCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCCTGCATGAAATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGCCGCATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAAGCGCAATACCTGCGTGCCCTCTCGAAGCCTCAG
GGCATGATCTTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCCCTCTATACTGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGACAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGAGAT
CTGGAGACCGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCATACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCAGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373