Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ397_RS20305 Genome accession   NZ_CP053883
Coordinates   4398473..4400179 (-) Length   568 a.a.
NCBI ID   WP_029302067.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 4960     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4393473..4405179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ397_RS20275 (HQ397_20195) mutT 4394149..4394562 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  HQ397_RS20280 (HQ397_20200) yacG 4394623..4394817 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  HQ397_RS20285 (HQ397_20205) zapD 4394827..4395549 (-) 723 WP_016352172.1 cell division protein ZapD -
  HQ397_RS20290 (HQ397_20210) coaE 4395587..4396201 (-) 615 WP_016352171.1 dephospho-CoA kinase -
  HQ397_RS20295 (HQ397_20215) pilD 4396221..4397093 (-) 873 WP_252376354.1 A24 family peptidase Machinery gene
  HQ397_RS20300 (HQ397_20220) pilC 4397116..4398357 (-) 1242 WP_252376355.1 type II secretion system F family protein Machinery gene
  HQ397_RS20305 (HQ397_20225) pilB 4398473..4400179 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HQ397_RS20310 (HQ397_20230) tapA 4400183..4400635 (-) 453 WP_252376356.1 type IVa pilus major pilin TapA -
  HQ397_RS20315 (HQ397_20235) nadC 4400958..4401815 (-) 858 WP_252376357.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HQ397_RS20320 (HQ397_20240) - 4401821..4402291 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  HQ397_RS20325 (HQ397_20245) ampD 4402439..4403011 (+) 573 WP_252376358.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HQ397_RS20330 (HQ397_20250) pdhR 4403287..4404051 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4342

>NTDB_id=448421 HQ397_RS20305 WP_029302067.1 4398473..4400179(-) (pilB) [Aeromonas hydrophila strain 4960]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=448421 HQ397_RS20305 WP_029302067.1 4398473..4400179(-) (pilB) [Aeromonas hydrophila strain 4960]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGCCAAGCCAAGGCGCAGCGCAAACCTTTTGTGACCTTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTGG
CAGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAT
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGACATACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCACTGCTGGTCGAGGAGAACA
AGCTCACCACGGCCATCGGCAAGCTGATGGAGAGCGATCAAGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCTGACGAGGGATCACGACTCGACGAGAGCGTCAACACGGCTGACGATGACGCACCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAACGCGGGGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGTATTCTGCATGAAATAGCGACGCCACCGGTCAATCTGGCCAACCGCTTCTCGGCTCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGTCTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATACCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCAACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACTCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTAGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTGGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGTGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373