Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQ399_RS20095 Genome accession   NZ_CP053881
Coordinates   4307213..4308919 (+) Length   568 a.a.
NCBI ID   WP_041210692.1    Uniprot ID   A0AAQ0J2H4
Organism   Aeromonas jandaei strain 4608     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4302213..4313919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQ399_RS20070 (HQ399_20060) pdhR 4303409..4304173 (-) 765 WP_033115373.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HQ399_RS20075 (HQ399_20065) ampD 4304452..4305021 (-) 570 WP_042033204.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HQ399_RS20080 (HQ399_20070) - 4305144..4305614 (+) 471 WP_201912989.1 retropepsin-like aspartic protease -
  HQ399_RS20085 (HQ399_20075) nadC 4305618..4306475 (+) 858 WP_215802168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HQ399_RS20090 (HQ399_20080) - 4306799..4307212 (+) 414 WP_215802170.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HQ399_RS20095 (HQ399_20085) pilB 4307213..4308919 (+) 1707 WP_041210692.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HQ399_RS20100 (HQ399_20090) pilC 4309064..4310305 (+) 1242 WP_215802171.1 type II secretion system F family protein Machinery gene
  HQ399_RS20105 (HQ399_20095) pilD 4310368..4311240 (+) 873 WP_215802173.1 A24 family peptidase Machinery gene
  HQ399_RS20110 (HQ399_20100) coaE 4311258..4311866 (+) 609 WP_215802175.1 dephospho-CoA kinase -
  HQ399_RS20115 (HQ399_20105) zapD 4311911..4312633 (+) 723 WP_215802177.1 cell division protein ZapD -
  HQ399_RS20120 (HQ399_20110) yacG 4312643..4312837 (+) 195 WP_209297368.1 DNA gyrase inhibitor YacG -
  HQ399_RS20125 (HQ399_20115) mutT 4312898..4313311 (-) 414 WP_215802179.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62537.94 Da        Isoelectric Point: 5.3215

>NTDB_id=448371 HQ399_RS20095 WP_041210692.1 4307213..4308919(+) (pilB) [Aeromonas jandaei strain 4608]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILESKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESESDALGMEDIDESEI
SDLEVSDESSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRMSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=448371 HQ399_RS20095 WP_041210692.1 4307213..4308919(+) (pilB) [Aeromonas jandaei strain 4608]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTCATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAAGCCAAGGCCCAGCGTAAACCCTTTGTAACCTTCCTGATCGAGAACGAGATCCTTGAGAGCAAGGCTCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAGCATCATGTGCTGCCTATCTACACGCAAGGGCACACCCTCTATATCGCCATGTCTGA
CCCGACCAATGTGTCGGCGCTGGAAGATTTCGGCTTCAGCTTTGGCCTGCATACCGAAGCGCTGCTGGTCGAAGAGAACA
AGCTCACAGCGGCAATAGGAAAATTGCTGGAGAGCGAAAGCGATGCCCTCGGAATGGAGGATATCGATGAATCTGAAATA
TCCGACCTCGAAGTATCCGATGAGAGCAGTCGACTGGATGAAAGCGTCAACACCGCTGATGATGATGCCCCAATAGTCAA
ATATATCAACAAGATCATGATGGATGCCATCAAGCGTGGCGCCTCCGACCTGCACTTTGAACCCTACGAGCAGAAATACC
GCATTCGCTTTCGTATCGACGGCATCCTGCATGAAGTGGCAACCCCGCCAGTCAATCTGGCCAACCGTTTCTCAGCACGC
CTCAAGGTCATGGCGCGGCTCGATATTGCAGAGCGGCGCCTGCCTCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGTAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAGAAGATCGTTATTCGTTTGCTCGACTCTTCGG
CTGCCCGACTCAACATTGAACAACTCGGTTTTGATGATCGGCAAAAGGCGCAATATCTGCGTGCTCTCTCGAAACCTCAA
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGTAAAACGGTCTCGCTCTATACCGGTCTCAATATTCTCAACACTAC
CGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CTGGGCTGACCTTTGCCAGTGCCCTGCGATCCTTTCTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAGATCCGCGAT
CTGGAGACGGCAGAAATAGCCATCAAGGCCGCCCAGACCGGCCACCTGGTACTCTCCACCTTGCACACCAACTCAGCCGC
CGAAACCCTCACCCGGATGATGAACATGGGGGTGCCAGCCTTTAATATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCACGCAAACTGTGTGACCACTGCAAGGCTCCCGAAGTAGTGCCGGAAGCAGAGTTACTGGAGCTGGGCTTTACT
CAGCAGCAACTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGCTCTGGTGGATACAAGGGGCGGGT
CGGCATCTACGAGATCATGCTGATGTCGGAAAACATCGCAAAGCTGATCATGCAGGGGGCCAACTCGCTACAAATAGCGG
CCATAGCCCAGAAAGAGGGGATGCGTACCTTACGTATGTCAGGATTGGAAAAGGCTCGCCTTGGCGTCACCAGTCTGGCA
GAGGTCAACCGGGTGACAACGAATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

100

0.558
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.596

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361