Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY17_RS02365 Genome accession   NZ_CP053816
Coordinates   492130..492555 (-) Length   141 a.a.
NCBI ID   WP_142609993.1    Uniprot ID   -
Organism   Vibrio cholerae strain SA7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487130..497555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY17_RS02345 (HPY17_02345) coaE 487631..488239 (-) 609 WP_162810836.1 dephospho-CoA kinase -
  HPY17_RS02350 (HPY17_02350) pilD 488236..489111 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  HPY17_RS02355 (HPY17_02355) pilC 489169..490395 (-) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  HPY17_RS02360 (HPY17_02360) pilB 490438..492126 (-) 1689 WP_175247443.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY17_RS02365 (HPY17_02365) pilA 492130..492555 (-) 426 WP_142609993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY17_RS02370 (HPY17_02370) nadC 492800..493690 (-) 891 WP_175245918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY17_RS02375 (HPY17_02375) ampD 493986..494531 (+) 546 WP_175245919.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY17_RS02380 (HPY17_02380) fldB 494702..495220 (-) 519 WP_000690112.1 flavodoxin FldB -
  HPY17_RS02385 (HPY17_02385) xerD 495358..496266 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY17_RS02390 (HPY17_02390) dsbC 496283..497035 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14586.67 Da        Isoelectric Point: 7.8085

>NTDB_id=447788 HPY17_RS02365 WP_142609993.1 492130..492555(-) (pilA) [Vibrio cholerae strain SA7G]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPAYKDYVTKSELASGFATIKSVITPAELYFQENGKISASASTVDVLGV
SSGANTLGELTITADNTIQFAHDAGAALGAKFVYTRSSGGWSCTYTQPSAVSAAAPKGCTP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=447788 HPY17_RS02365 WP_142609993.1 492130..492555(-) (pilA) [Vibrio cholerae strain SA7G]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTAATGATTGTGGTAGCGATTATTGGTGTGTT
GGCAGCGATTGCTGTTCCTGCTTATAAGGATTATGTAACCAAGAGCGAATTGGCATCTGGTTTCGCTACTATCAAGTCCG
TGATAACTCCAGCTGAACTTTATTTCCAAGAAAATGGAAAAATAAGTGCAAGCGCAAGCACTGTTGATGTTCTCGGTGTA
TCTTCTGGAGCAAATACTTTAGGTGAACTTACTATAACCGCTGACAATACAATTCAATTTGCTCATGATGCTGGTGCTGC
ATTAGGTGCAAAATTTGTTTACACAAGAAGCTCTGGAGGTTGGAGTTGCACTTACACACAACCATCAGCAGTTAGTGCAG
CAGCTCCTAAGGGCTGTACACCATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.414

100

0.539
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.871

100

0.482
  pilA Vibrio cholerae strain A1552

43.871

100

0.482
  pilA Vibrio cholerae C6706

43.871

100

0.482
  pilA Pseudomonas aeruginosa PAK

39.735

100

0.426
  pilA Acinetobacter baumannii strain A118

39.189

100

0.411
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.695

92.908

0.369
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.681

97.872

0.369