Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPY13_RS02330 Genome accession   NZ_CP053796
Coordinates   484128..485816 (-) Length   562 a.a.
NCBI ID   WP_108243691.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL4G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479128..490816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY13_RS02305 (HPY13_02305) yacG 480194..480391 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  HPY13_RS02310 (HPY13_02310) zapD 480549..481289 (-) 741 WP_000207193.1 cell division protein ZapD -
  HPY13_RS02315 (HPY13_02315) coaE 481321..481929 (-) 609 WP_069216898.1 dephospho-CoA kinase -
  HPY13_RS02320 (HPY13_02320) pilD 481926..482801 (-) 876 WP_033932898.1 A24 family peptidase Machinery gene
  HPY13_RS02325 (HPY13_02325) pilC 482859..484085 (-) 1227 WP_000648506.1 type II secretion system F family protein Machinery gene
  HPY13_RS02330 (HPY13_02330) pilB 484128..485816 (-) 1689 WP_108243691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY13_RS02335 (HPY13_02335) pilA 485820..486248 (-) 429 WP_094393042.1 pilin Machinery gene
  HPY13_RS02340 (HPY13_02340) nadC 486493..487383 (-) 891 WP_094393041.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY13_RS02345 (HPY13_02345) ampD 487685..488230 (+) 546 WP_094393039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY13_RS02350 (HPY13_02350) fldB 488379..488897 (-) 519 WP_069503252.1 flavodoxin FldB -
  HPY13_RS02355 (HPY13_02355) xerD 489035..489943 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  HPY13_RS02360 (HPY13_02360) dsbC 489960..490712 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62083.19 Da        Isoelectric Point: 6.1395

>NTDB_id=447363 HPY13_RS02330 WP_108243691.1 484128..485816(-) (pilB) [Vibrio cholerae strain SL4G]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGFFPPRELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=447363 HPY13_RS02330 WP_108243691.1 484128..485816(-) (pilB) [Vibrio cholerae strain SL4G]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCGACGCAAGAACAAGCGGTGGTTACCCAGGT
TAGCGCTTCGGGGACTTCAGTGCCCGAGGCGCTGCTTGGTTTAGGTTTTTTCCCCCCCCGAGAACTCACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTTGGGCTACGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTGGCGGTCTCTGACCCCACATTACT
GCAAGCCGAAGAAGAGTTTCGCTTTGCCACAGGTTTACAAGTTGAACTGGCACTAGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGCTCGATTCAAGGCGCCGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCACT
CTGGTTAAAGTCAGTGATGACGAGCTACAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTACTGCTCGATGCGGTGCGTAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATTTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATAAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGTGATACCGCGAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAGATCGCGGTAAAAGCCGCACAAACCGGTCACTTGGTGCTTTCCACCCTGCACACCAACTCAGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCCTCACTCAGTTTAATCATCGCCCAGCGCCTCGCGC
GCCGCCTATGCAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGTCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGATATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTACAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.754

100

0.988
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50

92.883

0.464
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.043

100

0.397
  pilF Thermus thermophilus HB27

39.668

96.441

0.383