Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GE021_RS00820 Genome accession   NZ_CP053792
Coordinates   142273..143307 (+) Length   344 a.a.
NCBI ID   WP_172774157.1    Uniprot ID   -
Organism   Streptococcus canis strain HL_77_1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137273..148307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GE021_RS00810 (GE021_000810) - 140947..141312 (+) 366 WP_125073632.1 DUF1033 family protein -
  GE021_RS00815 (GE021_000815) comYA 141399..142340 (+) 942 WP_125073631.1 competence type IV pilus ATPase ComGA Machinery gene
  GE021_RS00820 (GE021_000820) comYB 142273..143307 (+) 1035 WP_172774157.1 competence type IV pilus assembly protein ComGB Machinery gene
  GE021_RS00825 (GE021_000825) comYC 143310..143636 (+) 327 WP_125073629.1 competence type IV pilus major pilin ComGC Machinery gene
  GE021_RS00830 (GE021_000830) comGD 143611..144039 (+) 429 WP_260436826.1 competence type IV pilus minor pilin ComGD -
  GE021_RS00835 (GE021_000835) comGE 144017..144292 (+) 276 WP_125073627.1 competence type IV pilus minor pilin ComGE -
  GE021_RS00840 (GE021_000840) comYF 144273..144713 (+) 441 WP_125073626.1 competence type IV pilus minor pilin ComGF Machinery gene
  GE021_RS00845 (GE021_000845) comGG 144691..145071 (+) 381 WP_125073625.1 competence type IV pilus minor pilin ComGG -
  GE021_RS00850 (GE021_000850) comYH 145121..146074 (+) 954 WP_125073624.1 class I SAM-dependent methyltransferase Machinery gene
  GE021_RS00855 (GE021_000855) - 146133..147329 (+) 1197 WP_093998734.1 acetate kinase -
  GE021_RS00860 (GE021_000860) - 147649..147855 (+) 207 WP_125073623.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39344.64 Da        Isoelectric Point: 9.0224

>NTDB_id=447248 GE021_RS00820 WP_172774157.1 142273..143307(+) (comYB) [Streptococcus canis strain HL_77_1]
MISYLQQDISVRNKQCLKKLSSKEQYKLIQLLTNLLSAGFQLTDIIAFLKKSQLVKPQYVLSMEDSLLKGRGLADMLAGL
GFSDAILTQISLADGHGDIKKTLFNIQNYLKQMTQLKRKTIEVITYPIMLLLFLFVIMLGLRHYLVPQLEETNQITDFLN
HFPTYFLGFGLALVLLLGVGWLRWRSQSRLNCLSHLSAFPFWGKLLQQYLTAYYAREWGTLIGQGLELPMILDIMATEKS
SLMQELAEDIRTSLLAGQAFPTKVATYPFFKKELSLMIEYGEIKSKLGQELEIYAQESWARFFSQLHQATQFIQPVIFLM
IALIIVMIYAAILLPIYQDMGGLI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=447248 GE021_RS00820 WP_172774157.1 142273..143307(+) (comYB) [Streptococcus canis strain HL_77_1]
TTGATCAGTTACTTGCAGCAGGACATCTCAGTCCGAAACAAGCAATGCTTGAAAAAATTGTCCAGTAAAGAGCAATATAA
GCTTATTCAGTTGTTGACTAATCTTCTATCAGCAGGATTTCAGTTGACAGATATTATTGCTTTTTTAAAGAAAAGTCAGC
TTGTGAAACCCCAGTATGTTCTTAGTATGGAAGATTCCCTTTTAAAGGGACGAGGCTTAGCAGATATGTTAGCTGGTCTG
GGATTTTCGGATGCCATCTTGACTCAGATTAGCCTAGCAGATGGGCACGGAGATATTAAGAAGACGCTGTTTAACATTCA
GAATTACCTGAAGCAAATGACCCAGCTCAAGCGAAAGACCATTGAAGTAATAACCTATCCAATAATGCTGTTGCTTTTTC
TCTTTGTCATCATGCTAGGATTGAGACACTATTTAGTGCCCCAATTAGAAGAGACAAACCAAATAACTGACTTTCTCAAT
CATTTTCCGACCTACTTTTTGGGTTTTGGTTTGGCTCTTGTGTTACTTTTGGGAGTGGGGTGGCTGAGATGGCGGTCTCA
AAGCCGTTTAAACTGTTTAAGCCACCTAAGTGCCTTTCCCTTTTGGGGAAAACTGTTACAACAATACCTAACCGCCTATT
ATGCAAGGGAATGGGGCACTTTGATAGGGCAGGGCCTTGAGTTACCGATGATTCTAGACATTATGGCAACCGAGAAATCC
TCACTGATGCAAGAATTGGCTGAAGATATTAGGACTAGTTTACTGGCAGGACAGGCTTTTCCTACCAAAGTAGCCACTTA
CCCTTTCTTTAAAAAAGAATTAAGTTTGATGATTGAATATGGAGAAATCAAATCCAAACTAGGTCAGGAGCTTGAAATCT
ATGCTCAAGAAAGTTGGGCTCGCTTTTTCAGCCAGCTTCATCAAGCAACGCAGTTTATTCAACCAGTTATTTTTTTAATG
ATTGCCTTAATAATCGTCATGATTTATGCGGCAATCTTATTACCAATTTACCAAGATATGGGAGGCCTTATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(29-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.097

98.547

0.523
  comYB Streptococcus mutans UA159

53.097

98.547

0.523
  comYB Streptococcus gordonii str. Challis substr. CH1

50

98.837

0.494
  comGB/cglB Streptococcus mitis NCTC 12261

46.291

97.965

0.453
  comGB/cglB Streptococcus mitis SK321

46.547

96.802

0.451
  comGB/cglB Streptococcus pneumoniae Rx1

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae D39

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae R6

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae TIGR4

44.745

96.802

0.433
  comGB Lactococcus lactis subsp. cremoris KW2

44

94.477

0.416