NTDB

Overview

Name	comEA/celA/cilE	Type	Machinery gene
Locus tag	I5W22_RS03145	Genome accession	NZ_CP065431
Coordinates	635955..636617 (+)	Length	220 a.a.
NCBI ID	WP_011922190.1	Uniprot ID	A0A0H3MVS9
Organism	Streptococcus suis strain YP20190405
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 630955..641617

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5W22_RS03125 (I5W22_03125)	-	631672..632601 (-)	930	WP_012775038.1	ABC transporter substrate-binding protein	-
I5W22_RS03130 (I5W22_03130)	-	632614..633399 (-)	786	WP_011922762.1	ABC transporter ATP-binding protein	-
I5W22_RS03135 (I5W22_03135)	-	633551..634996 (-)	1446	WP_011922188.1	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L- lysine ligase	-
I5W22_RS03140 (I5W22_03140)	-	635143..635892 (+)	750	WP_012775039.1	lysophospholipid acyltransferase family protein	-
I5W22_RS03145 (I5W22_03145)	comEA/celA/cilE	635955..636617 (+)	663	WP_011922190.1	helix-hairpin-helix domain-containing protein	Machinery gene
I5W22_RS03150 (I5W22_03150)	comEC/celB	636601..638838 (+)	2238	WP_012775040.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I5W22_RS03155 (I5W22_03155)	-	638855..638980 (+)	126	Protein_575	IS982 family transposase	-
I5W22_RS03160 (I5W22_03160)	-	639163..639819 (+)	657	WP_011922763.1	CBS domain-containing protein	-
I5W22_RS03165 (I5W22_03165)	-	639913..640740 (+)	828	WP_011922194.1	hypothetical protein	-

Sequence

Protein

Download Length: 220 a.a. Molecular weight: 23410.01 Da Isoelectric Point: 4.0676

                                >NTDB_id=447094 I5W22_RS03145 WP_011922190.1 635955..636617(+) (comEA/celA/cilE) [Streptococcus suis strain YP20190405]

MDTIKTYIEMLKEYKWQIALPAVAGLLMATFLIFSQPAKSDQTGLTDFPQTEQASSSQEQTEETSTEESEELSQLTVDVK

GAVEKPGLYTLEAGARVNDAVEAAGGLTSQADPKSINLAQKLSDEAVVYVASKDENISVVASTTASSAMSPEEKSTSLVN

LNTATEADLQTISGIGAKRAADIIAYREANGGFKSVNDLNNVSGIGDKTMESIRPYVTVE

Nucleotide

Download Length: 663 bp

                                >NTDB_id=447094 I5W22_RS03145 WP_011922190.1 635955..636617(+) (comEA/celA/cilE) [Streptococcus suis strain YP20190405]

ATGGATACGATTAAAACTTATATAGAAATGCTTAAAGAATACAAGTGGCAAATTGCTCTGCCAGCAGTGGCTGGCTTGCT

AATGGCGACGTTTTTAATATTCAGCCAACCTGCTAAGTCTGATCAAACAGGACTGACAGATTTTCCTCAAACCGAGCAAG

CTTCTAGCAGTCAGGAGCAGACGGAGGAAACCAGTACAGAAGAGAGTGAGGAGCTCAGCCAGCTAACCGTTGATGTCAAA

GGAGCGGTGGAAAAGCCTGGGTTATATACTTTAGAAGCTGGTGCGCGTGTCAATGACGCAGTTGAAGCAGCTGGCGGCTT

GACCAGTCAGGCAGACCCCAAGTCTATCAATCTGGCTCAGAAGCTCAGCGATGAGGCGGTGGTCTATGTAGCCAGCAAGG

ACGAAAACATCTCGGTGGTGGCCAGCACGACTGCCAGCTCTGCTATGTCTCCAGAAGAAAAAAGCACCAGTCTGGTCAAT

CTGAATACGGCGACTGAGGCGGACTTGCAGACCATTTCGGGTATCGGTGCCAAGCGGGCGGCGGACATTATCGCCTATCG

TGAGGCAAACGGTGGCTTCAAGTCGGTGAACGACCTCAACAATGTTTCGGGCATTGGCGACAAGACCATGGAAAGCATTC

GGCCTTATGTCACGGTTGAGTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(77-130)

Helix-hairpin-helix motif (HHH_3)

(157-218)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3MVS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae Rx1	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae D39	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus pneumoniae R6	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.945	99.545	0.477
comEA/celA/cilE	Streptococcus mitis SK321	46.637	100	0.473
comEA	Streptococcus thermophilus LMD-9	59.259	73.636	0.436
comEA	Lactococcus lactis subsp. cremoris KW2	37.788	98.636	0.373
comEA	Bacillus subtilis subsp. subtilis str. 168	37.559	96.818	0.364

Detailed information NTG0447094