Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MARHY_RS12225 Genome accession   NC_017067
Coordinates   2625954..2627672 (+) Length   572 a.a.
NCBI ID   WP_014422081.1    Uniprot ID   -
Organism   Marinobacter nauticus ATCC 49840     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2620954..2632672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MARHY_RS12205 (MARHY2561) - 2622309..2622920 (+) 612 WP_014422077.1 c-type cytochrome -
  MARHY_RS12210 (MARHY2562) - 2622930..2623379 (-) 450 WP_014422078.1 pilin -
  MARHY_RS12215 (MARHY2563) - 2623455..2624930 (-) 1476 WP_158011890.1 O-antigen ligase -
  MARHY_RS12220 (MARHY2564) - 2625061..2625558 (-) 498 WP_041655568.1 pilin -
  MARHY_RS12225 (MARHY2565) pilB 2625954..2627672 (+) 1719 WP_014422081.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MARHY_RS12230 (MARHY2566) pilC 2627675..2628895 (+) 1221 WP_014422082.1 type II secretion system F family protein Machinery gene
  MARHY_RS12235 (MARHY2567) pilD 2628965..2629840 (+) 876 WP_014422083.1 A24 family peptidase Machinery gene
  MARHY_RS12240 (MARHY2568) coaE 2629841..2630458 (+) 618 WP_014422084.1 dephospho-CoA kinase -
  MARHY_RS12245 (MARHY2569) - 2630547..2631536 (+) 990 WP_014422085.1 hypothetical protein -
  MARHY_RS12250 (MARHY2570) tsaA 2631545..2632294 (+) 750 WP_014422086.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63185.04 Da        Isoelectric Point: 4.8248

>NTDB_id=44709 MARHY_RS12225 WP_014422081.1 2625954..2627672(+) (pilB) [Marinobacter nauticus ATCC 49840]
MATNNRNVTLTGLARRFVDDGLLDEETAKDAFLQASQNRIPLITYLTQNQLADSSKLAFSAAMEFGVSVMDLDAFLPEMM
PEKVVDEKLLRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRAAIDRYLESHDSTMGDLEDA
DLEGVETESGDHEDDDGSVSTTEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKAYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKLKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAQLGIDVLGYEEDQKEMYLEAL
EQPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCNSCKQPSDVPQDVLLK
EGFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVRITDELANMIMEEASSIKIAKQAQAEGFRNLRQSALMKVIQGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=44709 MARHY_RS12225 WP_014422081.1 2625954..2627672(+) (pilB) [Marinobacter nauticus ATCC 49840]
ATGGCGACCAATAACAGAAACGTGACACTGACAGGACTTGCACGCCGCTTCGTAGACGACGGCCTGCTGGATGAAGAAAC
AGCAAAGGATGCATTTCTCCAGGCCTCCCAGAACCGCATTCCACTGATCACCTATCTGACACAGAATCAGCTGGCTGACA
GTTCCAAGCTCGCCTTCTCAGCGGCGATGGAATTTGGCGTTTCCGTAATGGATCTTGATGCGTTTCTGCCTGAGATGATG
CCTGAGAAGGTTGTCGACGAAAAACTACTCCGCAAACACAACGCCCTGCCGCTCTACAAACGCGGGAATCGCCTGTTTAT
CGCGGTGTCGGACCCCACGAACATTCAGGCCCTGGACGAGATCAAGTTCAATACTGGCCTGAGCACCGATGCCATCCTCG
TAGACGACGCCAAGCTACGGGCTGCCATCGATCGCTATCTGGAATCCCATGATTCCACCATGGGCGACCTTGAAGATGCA
GACCTCGAGGGAGTTGAGACGGAGAGCGGCGATCACGAAGATGACGACGGTTCCGTATCGACAACCGAAGTTGACGATGC
CCCGATCGTTAAGTATGTGAACAAAATGCTGCTGGACGCTATCCGTGGTGGCGCCTCCGATATTCACTTCGAGCCTTATG
AAAAAGCGTACCGGGTTCGATACCGTACAGACGGCATTCTTAAAGAAGTATCCCGCCCTTCCATCAAGCTGGCACCGAAG
ATTTCAGCGCGGGTGAAGATCATGGCGCAACTGGATATTTCTGAGCGCCGAGTCCCCCAGGACGGCCGGATAAAGCTGAA
GTTATCCAAAACCAAAGCCATCGACTTCCGGGTGAACACCCTCCCGACCCTGTGGGGAGAGAAGATTGTTCTGCGGATTC
TGGATCCCAGCCAGGCCCAGCTCGGGATTGACGTGCTGGGCTACGAAGAAGACCAGAAGGAAATGTATCTTGAGGCGCTC
GAGCAGCCTCAGGGCATGATCCTGGTAACTGGCCCGACCGGATCTGGTAAGACGGTATCACTCTACACCGGCCTTAATAT
TCTGAACACCACCGAGCGGAATATATCGACCGCTGAGGATCCGGCGGAGATCAACCTTGAGGGTATCAACCAGGTAAACG
TCAACACCAAGGTAGGCCTTGGGTTTGCCGAGGCCTTGCGCGCATTCCTGCGTCAGGATCCGGACGTCATCATGGTGGGT
GAGATCCGGGACCTTGAAACTGCAAACATCGCCATCAAAGCCGCACAGACCGGTCACCTGGTTCTATCTACACTGCACAC
CAACAGCGCCGCCGAGACGCTGACCCGGATGATGAACATGGGCGTGCCTGCCTTTAACATCGCAACGTCGGTTAGCCTGA
TCATCGCCCAACGACTTGGCCGAAGACTGTGCAACAGTTGCAAGCAACCCTCAGACGTCCCTCAGGACGTGCTTTTGAAG
GAAGGGTTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGTGGATA
TAAGGGCCGCGTCGGTATTTACGAGGTGGTCCGCATTACCGATGAACTGGCAAACATGATTATGGAAGAAGCCAGCTCCA
TTAAAATTGCAAAGCAGGCTCAGGCAGAAGGCTTCCGAAATCTCAGGCAGTCGGCTCTTATGAAGGTGATTCAGGGAGTG
ACCAGCCTTGAGGAAGCCAATCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.326

100

0.568
  pilB Acinetobacter baylyi ADP1

56.915

98.601

0.561
  pilB Legionella pneumophila strain ERS1305867

54.167

100

0.545
  pilB Vibrio parahaemolyticus RIMD 2210633

52.33

97.552

0.51
  pilB Vibrio campbellii strain DS40M4

50.619

98.776

0.5
  pilB Vibrio cholerae strain A1552

54.99

89.336

0.491
  pilF Neisseria gonorrhoeae MS11

48.148

99.126

0.477
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

92.133

0.374
  pilF Thermus thermophilus HB27

37.726

96.853

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

40.347

90.559

0.365

Multiple sequence alignment