Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   C270_RS02155 Genome accession   NC_018673
Coordinates   402487..403122 (+) Length   211 a.a.
NCBI ID   WP_014973916.1    Uniprot ID   K0DCT0
Organism   Leuconostoc carnosum JB16     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 397487..408122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C270_RS02130 (C270_01900) - 398100..398891 (+) 792 WP_014973911.1 metal ABC transporter permease -
  C270_RS02135 (C270_01905) purR 398993..399826 (+) 834 WP_014973912.1 pur operon repressor -
  C270_RS02140 (C270_01910) glmU 399833..401209 (+) 1377 WP_014973913.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  C270_RS02145 (C270_01915) - 401341..401832 (+) 492 WP_014973914.1 hypothetical protein -
  C270_RS02150 (C270_01920) - 401842..402099 (-) 258 WP_014973915.1 hypothetical protein -
  C270_RS02155 (C270_01925) comEA/celA/cilE 402487..403122 (+) 636 WP_014973916.1 helix-hairpin-helix domain-containing protein Machinery gene
  C270_RS02160 (C270_01930) - 403122..405407 (+) 2286 WP_014973917.1 DNA internalization-related competence protein ComEC/Rec2 -
  C270_RS02165 (C270_01935) holA 405376..406377 (+) 1002 WP_014973918.1 DNA polymerase III subunit delta -
  C270_RS02170 (C270_01940) rpsT 406497..406748 (-) 252 WP_014973919.1 30S ribosomal protein S20 -
  C270_RS02175 (C270_01945) rpsO 406798..407067 (-) 270 WP_013103687.1 30S ribosomal protein S15 -
  C270_RS02180 (C270_01950) gatC 407298..407606 (+) 309 WP_014973920.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -

Sequence

Protein


Download         Length: 211 a.a.        Molecular weight: 22748.96 Da        Isoelectric Point: 6.7325

>NTDB_id=44647 C270_RS02155 WP_014973916.1 402487..403122(+) (comEA/celA/cilE) [Leuconostoc carnosum JB16]
MMDTFDVIKNKLADYKILIAIVFVILVSGIFLLVHTRTENTPVAAYSAKAVSSVSDTVSSTSSTVKSTTAIMVDIKGAVK
TPGVYDIIDNPRVQTLIARSGGVTAQADINQVNLAQKLTDGQMIYIPVKGERLPNHSTANTPNTNSNQTQVNLNTATIEE
LQTLEGVGAKKAEQIIAYRDEHGGFKSIEEIKQVAGVGDKRFEAIQDKITV

Nucleotide


Download         Length: 636 bp        

>NTDB_id=44647 C270_RS02155 WP_014973916.1 402487..403122(+) (comEA/celA/cilE) [Leuconostoc carnosum JB16]
ATGATGGATACATTTGATGTCATAAAAAATAAATTAGCTGATTATAAGATATTAATAGCTATAGTTTTCGTGATTTTAGT
TAGCGGGATTTTTCTTTTAGTGCACACTAGAACAGAAAATACCCCAGTAGCAGCTTATAGTGCCAAGGCTGTGAGTAGTG
TTAGCGATACGGTTAGCAGCACGTCATCTACTGTTAAATCAACTACTGCGATTATGGTTGATATTAAAGGTGCTGTTAAG
ACGCCTGGTGTTTATGATATTATTGATAACCCACGCGTGCAAACTTTGATTGCTAGATCAGGTGGCGTGACTGCACAAGC
AGACATAAACCAAGTCAACTTAGCGCAAAAATTGACTGATGGTCAAATGATTTACATACCGGTGAAAGGTGAGCGTTTAC
CAAATCATTCAACAGCCAATACACCAAATACTAATAGTAATCAGACTCAGGTTAATTTAAACACGGCCACAATTGAGGAA
TTACAAACTTTAGAGGGTGTTGGCGCAAAAAAAGCTGAACAAATTATTGCTTACCGTGACGAACATGGTGGTTTTAAATC
GATTGAAGAAATTAAACAAGTTGCTGGGGTTGGTGATAAACGTTTCGAAGCCATCCAAGATAAAATAACAGTCTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(72-127)

Helix-hairpin-helix motif (HHH_3)

(146-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0DCT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae Rx1

39.171

100

0.403
  comEA/celA/cilE Streptococcus pneumoniae D39

39.171

100

0.403
  comEA/celA/cilE Streptococcus pneumoniae R6

39.171

100

0.403
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

38.71

100

0.398
  comEA Latilactobacillus sakei subsp. sakei 23K

37.22

100

0.393
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.249

100

0.393
  comEA/celA/cilE Streptococcus mitis SK321

38.426

100

0.393
  comEA Lactococcus lactis subsp. cremoris KW2

37.037

100

0.379
  comEA Bacillus subtilis subsp. subtilis str. 168

39.899

93.839

0.374
  comEA Streptococcus thermophilus LMD-9

34.222

100

0.365

Multiple sequence alignment