Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HPK20_RS12255 Genome accession   NZ_CP053664
Coordinates   2655746..2656972 (+) Length   408 a.a.
NCBI ID   WP_171933911.1    Uniprot ID   -
Organism   Vibrio fluvialis strain A8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2650746..2661972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK20_RS12230 (HPK20_12250) fldB 2651214..2651735 (+) 522 WP_171933908.1 flavodoxin FldB -
  HPK20_RS12235 (HPK20_12255) ampD 2651771..2652325 (-) 555 WP_171933909.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPK20_RS12240 (HPK20_12260) nadC 2652478..2653365 (+) 888 WP_171933910.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPK20_RS12245 (HPK20_12265) pilA 2653597..2654016 (+) 420 WP_024374400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPK20_RS12250 (HPK20_12270) pilB 2654022..2655710 (+) 1689 WP_171934101.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPK20_RS12255 (HPK20_12275) pilC 2655746..2656972 (+) 1227 WP_171933911.1 type II secretion system F family protein Machinery gene
  HPK20_RS12260 (HPK20_12280) pilD 2657024..2657893 (+) 870 WP_171933912.1 A24 family peptidase Machinery gene
  HPK20_RS12265 (HPK20_12285) coaE 2657896..2658504 (+) 609 WP_171933913.1 dephospho-CoA kinase -
  HPK20_RS12270 (HPK20_12290) zapD 2658537..2659277 (+) 741 WP_020332196.1 cell division protein ZapD -
  HPK20_RS12275 (HPK20_12295) yacG 2659332..2659529 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  HPK20_RS12280 (HPK20_12300) - 2659627..2660831 (+) 1205 WP_171933914.1 IS3 family transposase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44700.94 Da        Isoelectric Point: 10.5318

>NTDB_id=446135 HPK20_RS12255 WP_171933911.1 2655746..2656972(+) (pilC) [Vibrio fluvialis strain A8]
MKPTKGPELKNYRWKGVNSTGKKVAGQTLAISEIEVRDKLKEQHIQVKKIKKGSVSVLTRLTNRVKTKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPISKAMRTASTHFDALYVDLIQTGEQSGNLAEVFERLATYRE
KSEQLRAKVIKALIYPSMVILVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQILKLSHGVQAYSAGAFIASLSLVFG
IKAARKKSFVVRLKTSQLSLRFPIIGGVLAKAAIAKFSRTLATSFSAGIPILASLKTTAKTAGNVHFETAIQDVHRHTAA
GMPMYIAMRNTQAFPEMVLQMVMIGEESGKLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGIVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=446135 HPK20_RS12255 WP_171933911.1 2655746..2656972(+) (pilC) [Vibrio fluvialis strain A8]
ATGAAACCGACCAAAGGCCCTGAGTTAAAAAACTATCGCTGGAAAGGCGTCAACAGCACTGGCAAGAAAGTCGCGGGACA
GACACTGGCTATCAGTGAAATAGAAGTCCGCGACAAACTGAAAGAACAGCACATTCAGGTTAAGAAGATCAAAAAAGGCA
GCGTGTCAGTTCTGACTCGTCTCACCAATCGGGTCAAAACTAAAGACATCACCATCCTAACCCGCCAGCTTGCCACCATG
CTGACCACCGGAGTGCCGATTGTGCAGGCACTTAAATTGGTCGGCGATAATCATCGTAAAGCCGAGATGAAATCGATTCT
GGCGCAAATTACCAAAGGCGTCGAAGCCGGTACGCCGATTTCCAAAGCGATGCGTACTGCCAGCACTCACTTTGACGCGT
TGTACGTCGACTTAATTCAGACTGGCGAGCAGTCCGGCAATCTTGCCGAAGTCTTTGAACGCCTTGCCACCTATCGGGAA
AAAAGCGAGCAACTGCGCGCGAAAGTAATCAAAGCGCTGATCTATCCCAGCATGGTCATTCTGGTGGCACTCGGCGTCTC
CTATTTGATGCTGACGATGGTCATTCCAGAGTTCGAAAGTATGTTCAAGGGTTTTGGGGCTGAACTGCCTTGGTTTACTC
AGCAGATCCTCAAGTTATCGCATGGGGTACAAGCCTACAGTGCGGGGGCGTTCATCGCGTCATTGAGCTTGGTGTTTGGC
ATCAAAGCGGCGCGGAAAAAATCCTTCGTGGTCCGTCTTAAAACCAGCCAGCTCAGCCTGCGTTTTCCCATCATTGGCGG
TGTGCTTGCCAAAGCCGCAATTGCCAAATTCAGCCGCACTCTGGCGACCAGCTTCAGTGCGGGAATTCCGATACTCGCCA
GCCTCAAAACCACCGCTAAAACGGCAGGTAACGTGCATTTTGAAACCGCCATTCAGGATGTTCACCGCCATACGGCGGCA
GGGATGCCGATGTATATTGCAATGCGCAACACACAAGCCTTTCCGGAAATGGTGTTGCAAATGGTGATGATCGGCGAGGA
GTCCGGCAAGCTCGACGACATGCTCAATAAAGTCGCCACCATCTATGAGTTTGAAGTCGACAACACCGTCGACAATCTGG
GCAAAATTCTTGAACCGCTGATTATCGTATTTTTGGGCATTGTGGTCGGTGGATTGGTGGTGGCGATGTACCTGCCAATC
TTTAACTTAATGAGTGTATTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

87.01

100

0.87
  pilC Vibrio campbellii strain DS40M4

72.593

99.265

0.721
  pilC Acinetobacter baumannii D1279779

41.667

100

0.417
  pilC Legionella pneumophila strain ERS1305867

41.103

97.794

0.402
  pilC Acinetobacter baylyi ADP1

41.31

97.304

0.402
  pilC Pseudomonas stutzeri DSM 10701

39.899

97.059

0.387
  pilG Neisseria gonorrhoeae MS11

39.599

97.794

0.387
  pilG Neisseria meningitidis 44/76-A

39.098

97.794

0.382