Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPK20_RS12245 Genome accession   NZ_CP053664
Coordinates   2653597..2654016 (+) Length   139 a.a.
NCBI ID   WP_024374400.1    Uniprot ID   -
Organism   Vibrio fluvialis strain A8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2648597..2659016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK20_RS12220 (HPK20_12240) dsbC 2649384..2650148 (-) 765 WP_055452474.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  HPK20_RS12225 (HPK20_12245) xerD 2650165..2651073 (-) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  HPK20_RS12230 (HPK20_12250) fldB 2651214..2651735 (+) 522 WP_171933908.1 flavodoxin FldB -
  HPK20_RS12235 (HPK20_12255) ampD 2651771..2652325 (-) 555 WP_171933909.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPK20_RS12240 (HPK20_12260) nadC 2652478..2653365 (+) 888 WP_171933910.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPK20_RS12245 (HPK20_12265) pilA 2653597..2654016 (+) 420 WP_024374400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPK20_RS12250 (HPK20_12270) pilB 2654022..2655710 (+) 1689 WP_171934101.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPK20_RS12255 (HPK20_12275) pilC 2655746..2656972 (+) 1227 WP_171933911.1 type II secretion system F family protein Machinery gene
  HPK20_RS12260 (HPK20_12280) pilD 2657024..2657893 (+) 870 WP_171933912.1 A24 family peptidase Machinery gene
  HPK20_RS12265 (HPK20_12285) coaE 2657896..2658504 (+) 609 WP_171933913.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14369.33 Da        Isoelectric Point: 8.4715

>NTDB_id=446133 HPK20_RS12245 WP_024374400.1 2653597..2654016(+) (pilA) [Vibrio fluvialis strain A8]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSEVASAVATMKSLITPAELYYQENGSFPSSSTELEKVGV
GSNAIKNGTLGFTSSAISLTGFSSPSSASVTITRNATSGWSCAVAGLTKADNAPKGCPQ

Nucleotide


Download         Length: 420 bp        

>NTDB_id=446133 HPK20_RS12245 WP_024374400.1 2653597..2654016(+) (pilA) [Vibrio fluvialis strain A8]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTCCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAAGTAGCATCTGCAGTAGCAACGATGAAGTCTT
TAATTACTCCAGCGGAGCTTTACTACCAAGAAAACGGCTCATTCCCAAGTTCAAGCACAGAATTGGAGAAAGTTGGTGTA
GGAAGTAACGCAATTAAGAATGGAACTTTAGGGTTTACATCCAGCGCAATCAGTCTTACAGGATTCAGCTCGCCATCATC
TGCTTCTGTTACAATAACAAGAAATGCCACTAGTGGTTGGTCTTGTGCCGTAGCTGGCCTCACTAAAGCAGATAATGCCC
CTAAAGGATGTCCGCAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

51.908

94.245

0.489
  pilA Pseudomonas aeruginosa PAK

39.735

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.662

100

0.417
  pilA Vibrio cholerae strain A1552

37.662

100

0.417
  pilA Vibrio cholerae C6706

37.662

100

0.417
  pilA Acinetobacter baumannii strain A118

37.415

100

0.396