Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HKK54_RS08425 Genome accession   NZ_CP052860
Coordinates   1786815..1788152 (-) Length   445 a.a.
NCBI ID   WP_169389260.1    Uniprot ID   -
Organism   Pseudomonas sp. ADAK13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1781815..1793152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKK54_RS08410 (HKK54_08410) - 1783480..1785033 (-) 1554 WP_169386557.1 AAA family ATPase -
  HKK54_RS08415 (HKK54_08415) aroB 1785044..1786144 (-) 1101 WP_169386558.1 3-dehydroquinate synthase -
  HKK54_RS08420 (HKK54_08420) aroK 1786292..1786810 (-) 519 WP_003209345.1 shikimate kinase AroK -
  HKK54_RS08425 (HKK54_08425) pilQ 1786815..1788152 (-) 1338 WP_169389260.1 type IV pilus secretin PilQ family protein Machinery gene
  HKK54_RS08430 (HKK54_08430) - 1788222..1789238 (-) 1017 WP_169386559.1 pilus assembly protein PilP -
  HKK54_RS08435 (HKK54_08435) - 1789235..1789786 (-) 552 WP_010169205.1 PilN domain-containing protein -
  HKK54_RS08440 (HKK54_08440) pilM 1789786..1790439 (-) 654 WP_010169204.1 type IV pilus assembly protein PilM -
  HKK54_RS08445 (HKK54_08445) - 1790657..1793116 (+) 2460 WP_010169203.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 48436.46 Da        Isoelectric Point: 4.7160

>NTDB_id=441859 HKK54_RS08425 WP_169389260.1 1786815..1788152(-) (pilQ) [Pseudomonas sp. ADAK13]
MVLAVPNRIDLIHLPPPGNVPLSTARVAFTGDKLSLNFQNIELRAVLQQIADVAGLNLVASDEVQGSITLRLKDVPWDQA
LDLVLQTKGLDKRVTANVLLVAPAEELAARELLVLESRKQMTELAPLRRELLQVNYAKAADLAKLFQSAVGLEGETDERG
SVAVDDRTNNIIAYQTQERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGQANRGNWGAGGIRTPLADGA
EPPESPPSSPFVDLGTVNGSAGLGVAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSTG
ATSVSFKEASLSLEVTPQITPDGWVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVD
KVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=441859 HKK54_RS08425 WP_169389260.1 1786815..1788152(-) (pilQ) [Pseudomonas sp. ADAK13]
ATGGTCCTGGCTGTGCCCAATCGAATCGACCTGATTCACCTGCCGCCACCTGGCAACGTGCCCCTGAGCACCGCACGGGT
TGCATTTACTGGCGACAAGCTGTCCCTCAATTTCCAGAACATCGAACTGCGTGCGGTGCTGCAGCAGATTGCCGATGTTG
CCGGCCTCAATCTGGTGGCCAGCGATGAGGTGCAGGGTTCGATCACCTTGCGGCTCAAAGACGTACCGTGGGACCAGGCT
CTGGACCTGGTGCTGCAAACCAAGGGGTTGGATAAGCGTGTGACGGCCAACGTGCTGCTGGTCGCGCCGGCCGAGGAGTT
GGCCGCTCGGGAATTGCTGGTGCTGGAGTCGCGCAAGCAAATGACTGAGCTGGCACCGCTGCGCCGCGAGTTGCTGCAGG
TCAACTACGCCAAGGCGGCGGACCTGGCCAAGCTGTTCCAGTCGGCCGTGGGCCTGGAAGGTGAAACCGATGAGCGGGGC
TCGGTGGCGGTGGATGACCGCACCAACAACATCATCGCCTACCAGACCCAGGAGCGCCTGGAGGAGCTGCGCCGGATAGT
GGCGCAGCTGGATATACCGGTGCGGCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAACGTGGATTACGACAAAAGTC
TCGGCGTGCGCTGGGGCGGGCAGGCCAACCGGGGGAACTGGGGCGCCGGCGGGATCAGGACGCCGCTGGCGGACGGGGCC
GAGCCGCCCGAAAGTCCGCCCAGCTCACCGTTTGTCGATCTGGGTACGGTCAACGGCAGCGCCGGCCTGGGTGTCGCCTT
CATCACCGACAACCTGCTGCTGGACCTGGAACTGACGGCCATGGAAAAAACCGGCAACGGGGAAATTGTCTCGCAGCCCA
AGGTCGTCACCTCCGACAAGGAAACCGCGCGAATCCTCAAGGGCACCGAGATTCCTTATCAGGAGTCCAGTTCTACCGGG
GCCACCTCGGTATCGTTCAAGGAGGCCTCCTTGTCATTGGAAGTGACCCCGCAAATCACCCCGGATGGCTGGGTGATCAT
GGAGGTCAAGGTCACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCA
ACGCCAAGGTGCTGGTCAAGGATGGGGAGACCATCGTGATTGGCGGGGTTTTCTCCAATACTCAAAGCAAAGTGGTAGAT
AAAGTGCCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGGGATGTGGTTTCGGAGAAAAAATCCGAGCT
GCTGGTATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Bacterial type II and III secretion system protein (Secretin)

(280-436)

Secretin and TonB N terminus short domain (STN)

(57-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.854

98.202

0.706
  comQ Acinetobacter baylyi ADP1

42.38

100

0.456
  pilQ Acinetobacter baumannii strain A118

42.457

100

0.443
  pilQ Acinetobacter baumannii D1279779

42.457

100

0.443
  pilQ Vibrio campbellii strain DS40M4

45.39

95.056

0.431
  pilQ Legionella pneumophila strain Lp02

45

94.382

0.425
  pilQ Legionella pneumophila strain ERS1305867

45

94.382

0.425
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.498

93.933

0.418
  pilQ Vibrio cholerae strain A1552

44.498

93.933

0.418