Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HED66_RS13750 Genome accession   NZ_CP051883
Coordinates   3035018..3036724 (+) Length   568 a.a.
NCBI ID   WP_058394588.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain SRW-OG1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3030018..3041724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HED66_RS13725 pdhR 3031188..3031952 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HED66_RS13730 ampD 3032229..3032801 (-) 573 WP_169047635.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HED66_RS13735 - 3032951..3033421 (+) 471 WP_011898292.1 retropepsin-like aspartic protease family protein -
  HED66_RS13740 nadC 3033426..3034283 (+) 858 WP_111588902.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HED66_RS13745 - 3034619..3035011 (+) 393 WP_083195368.1 pilin -
  HED66_RS13750 pilB 3035018..3036724 (+) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HED66_RS13755 pilC 3036853..3038094 (+) 1242 WP_021140339.1 type II secretion system F family protein Machinery gene
  HED66_RS13760 pilD 3038158..3039033 (+) 876 WP_058394589.1 prepilin peptidase Machinery gene
  HED66_RS13765 coaE 3039053..3039667 (+) 615 WP_169046904.1 dephospho-CoA kinase -
  HED66_RS13770 zapD 3039705..3040427 (+) 723 WP_169046905.1 cell division protein ZapD -
  HED66_RS13775 yacG 3040437..3040631 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  HED66_RS13780 mutT 3040709..3041116 (-) 408 WP_111588905.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=441327 HED66_RS13750 WP_058394588.1 3035018..3036724(+) (pilB) [Aeromonas salmonicida strain SRW-OG1]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=441327 HED66_RS13750 WP_058394588.1 3035018..3036724(+) (pilB) [Aeromonas salmonicida strain SRW-OG1]
GTGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCTCAGCGCAAACCCTTTGTCACTTTTCTCATTGAAAACGAAATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACAGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCAGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAAATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGTTTTCGGATCGATGGCATACTGCACGAGATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CGGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAAGCACAGTATCTACGTGCCCTCTCCAAGCCCCAG
GGCATGATTCTGGTGACAGGTCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAATACCAC
AGAGGTCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAGGTAGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAATATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CCATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375