Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HED66_RS13760 Genome accession   NZ_CP051883
Coordinates   3038158..3039033 (+) Length   291 a.a.
NCBI ID   WP_058394589.1    Uniprot ID   A0AAX1PH66
Organism   Aeromonas salmonicida strain SRW-OG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3033158..3044033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HED66_RS13740 nadC 3033426..3034283 (+) 858 WP_111588902.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HED66_RS13745 - 3034619..3035011 (+) 393 WP_083195368.1 pilin -
  HED66_RS13750 pilB 3035018..3036724 (+) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HED66_RS13755 pilC 3036853..3038094 (+) 1242 WP_021140339.1 type II secretion system F family protein Machinery gene
  HED66_RS13760 pilD 3038158..3039033 (+) 876 WP_058394589.1 prepilin peptidase Machinery gene
  HED66_RS13765 coaE 3039053..3039667 (+) 615 WP_169046904.1 dephospho-CoA kinase -
  HED66_RS13770 zapD 3039705..3040427 (+) 723 WP_169046905.1 cell division protein ZapD -
  HED66_RS13775 yacG 3040437..3040631 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  HED66_RS13780 mutT 3040709..3041116 (-) 408 WP_111588905.1 8-oxo-dGTP diphosphatase MutT -
  HED66_RS13785 secA 3041283..3044003 (-) 2721 WP_169046906.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32423.70 Da        Isoelectric Point: 7.0169

>NTDB_id=441329 HED66_RS13760 WP_058394589.1 3038158..3039033(+) (pilD) [Aeromonas salmonicida strain SRW-OG1]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEADERYNLMVPRSCCPHCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTVL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=441329 HED66_RS13760 WP_058394589.1 3038158..3039033(+) (pilD) [Aeromonas salmonicida strain SRW-OG1]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTATTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAATGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGCTGACGAGCGCTACAACCTGATGGTGCCGCGCTCCTGCTGCCCTCACTGCAATCAC
CCCATAACGGCACTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCCCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCAGGCTGGG
GCACACTGGCTGCTCTTCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTGCTGTGGGGCGGGCTGCTATTCAACCTGCTTGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCGGCGCTGGGAGCCTGGCTTGGCTGGCAGGCACTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTGTCCTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.111

98.969

0.605
  pilD Vibrio campbellii strain DS40M4

55.208

98.969

0.546
  pilD Acinetobacter baumannii D1279779

55.019

92.44

0.509
  pilD Acinetobacter nosocomialis M2

55.019

92.44

0.509
  pilD Neisseria gonorrhoeae MS11

50.373

92.096

0.464