Detailed information NTG0441227

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HIN87_RS04650
• Genome accession: NZ_CP051869
• Coordinates: 946022..947080 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain Ab-D10a-a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 941022..952080

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIN87_RS04625 (HIN87_04625)	aroK	941297..941866 (-)	570	WP_000643712.1	shikimate kinase AroK	-
HIN87_RS04630 (HIN87_04630)	pilQ	941878..944043 (-)	2166	WP_031999626.1	type IV pilus secretin PilQ family protein	Machinery gene
HIN87_RS04635 (HIN87_04635)	pilP	944106..944633 (-)	528	WP_057049054.1	pilus assembly protein PilP	Machinery gene
HIN87_RS04640 (HIN87_04640)	pilO	944644..945384 (-)	741	WP_031999622.1	type 4a pilus biogenesis protein PilO	Machinery gene
HIN87_RS04645 (HIN87_04645)	pilN	945381..946022 (-)	642	WP_169030851.1	PilN domain-containing protein	Machinery gene
HIN87_RS04650 (HIN87_04650)	pilM	946022..947080 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
HIN87_RS04655 (HIN87_04655)	ponA	947242..949797 (+)	2556	WP_031999621.1	penicillin-binding protein PBP1a	-
HIN87_RS04660 (HIN87_04660)	-	949815..950639 (-)	825	WP_002019666.1	putative RNA methyltransferase	-
HIN87_RS04665 (HIN87_04665)	-	950642..950974 (-)	333	WP_000169603.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=441227 HIN87_RS04650 WP_002014066.1 946022..947080(-) (pilM) [Acinetobacter baumannii strain Ab-D10a-a]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=441227 HIN87_RS04650 WP_002014066.1 946022..947080(-) (pilM) [Acinetobacter baumannii strain Ab-D10a-a]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTATGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423