Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HG421_RS13570 Genome accession   NZ_CP051651
Coordinates   3214515..3216248 (-) Length   577 a.a.
NCBI ID   WP_169706819.1    Uniprot ID   A0A7Z2ZHT9
Organism   Xanthomonas campestris pv. badrii strain NEB122     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3209515..3221248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG421_RS13545 (HG421_13545) - 3210464..3212077 (+) 1614 WP_169706815.1 HAMP domain-containing sensor histidine kinase -
  HG421_RS13550 (HG421_13550) pilR 3212393..3213787 (+) 1395 WP_169706816.1 sigma-54 dependent transcriptional regulator Regulator
  HG421_RS13555 (HG421_13555) - 3213947..3214135 (-) 189 WP_169706817.1 hypothetical protein -
  HG421_RS13560 (HG421_13560) pilB 3214137..3214256 (-) 120 WP_029220459.1 hypothetical protein Machinery gene
  HG421_RS13565 (HG421_13565) - 3214325..3214513 (-) 189 WP_169706818.1 hypothetical protein -
  HG421_RS13570 (HG421_13570) pilB 3214515..3216248 (-) 1734 WP_169706819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HG421_RS21435 (HG421_13575) pilA2 3216291..3216704 (-) 414 WP_169706820.1 pilin Machinery gene
  HG421_RS13580 (HG421_13580) comP 3216804..3217229 (-) 426 WP_169706821.1 pilin Machinery gene
  HG421_RS13585 (HG421_13585) pilC 3217588..3218847 (+) 1260 WP_169706822.1 type II secretion system F family protein Machinery gene
  HG421_RS13590 (HG421_13590) - 3218854..3219717 (+) 864 WP_169706823.1 A24 family peptidase -
  HG421_RS13595 (HG421_13595) coaE 3219731..3220339 (+) 609 WP_169706824.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62793.82 Da        Isoelectric Point: 5.7611

>NTDB_id=440250 HG421_RS13570 WP_169706819.1 3214515..3216248(-) (pilB) [Xanthomonas campestris pv. badrii strain NEB122]
MNAASNFNLVGITGIARRLVQDGALDEASARVAMDQAAAAKTPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDEGMDNLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=440250 HG421_RS13570 WP_169706819.1 3214515..3216248(-) (pilB) [Xanthomonas campestris pv. badrii strain NEB122]
ATGAATGCCGCGAGCAATTTTAATCTGGTTGGAATTACAGGTATCGCACGCCGCTTGGTGCAAGATGGAGCGTTGGATGA
AGCCAGCGCCCGTGTTGCCATGGATCAAGCTGCGGCGGCGAAAACTCCGCTGCCACAATGGTTCTCCGAGAAAAAGCTGG
TTTCAGCGTCTCAACTTGCTGCCGCCAACGCAGTCGAGTTCGGCATGCCCTTGATGGATGTGTCGGTATTCGACGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGAGTAAGCAACCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTCCATACGAATCTGGTGGTCGAGCCAA
TTTTGGTCGACGAGGATCAGATCCGTCGCACGCTTGAGCAGTGGCAGGCGAGCAATGCGTCGCTTGGGTCGTCGCTCGGT
GACGATGATGAGGGCATGGACAACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCCGACA
TCCATTTCGAACCTTATGAAGACGACTATCGGGTGCGCTTGCGCATCGATGGACTGCTCAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAATCAGCGCATCGCGGCACGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTGCCGCAAGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATTCTGGATGGCAGTGCTGCCAAGCTGGGTATCGACAAGCTTGGTTATGAGGCGGACCAGCAGAAG
TTGTTCCTGGATGCGATCCACAAGCCTTACGGGATGGTGCTGGTTACCGGGCCGACTGGCTCGGGTAAGACGGTGTCGCT
GTACACGGCGCTGGGTATTCTCAACGACGAGACGCGCAATATCTCCACGGCTGAGGATCCGGTCGAAATTCGCTTGCCCG
GCGTCAATCAGGTTCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCCTTGCGTTCGTTTCTGCGCCAGGACCCC
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCATATGGT
GCTGTCGACACTGCACACCAATGACGCCCCGCAGACCATCGCGCGTTTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATCGCGCAGCGGCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGTAAGTCGACCTTGCCG
GAGCATGCGCTGCTGGCGGAAGGGTTCACGCCAGAGCAAGTTGCGAACATCGAGCTCTATGAAGCTGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGTGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGCATCCGCGACTTGCGTCAGTCGGCGCTGATGAAG
GCTGCGCATGGGGTGACTAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2ZHT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.063

98.614

0.553
  pilB Acinetobacter baylyi ADP1

55.634

98.44

0.548
  pilB Legionella pneumophila strain ERS1305867

52.641

98.44

0.518
  pilB Vibrio cholerae strain A1552

49.132

99.827

0.49
  pilF Neisseria gonorrhoeae MS11

49.472

98.44

0.487
  pilB Vibrio campbellii strain DS40M4

46.397

98.614

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

47.048

96.88

0.456