Detailed information NTG0439719

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HHA37_RS23545
• Genome accession: NZ_CP051547
• Coordinates: 5089549..5091249 (+)
• Length: 566 a.a.
• NCBI ID: WP_122359259.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain AA2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5091253..5099953	5089549..5091249	flank	4

Gene organization within MGE regions

Location: 5089549..5099953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HHA37_RS23545 (HHA37_23580)	pilB	5089549..5091249 (+)	1701	WP_122359259.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HHA37_RS23550 (HHA37_23585)	pilC	5091253..5092470 (+)	1218	WP_010792214.1	type II secretion system F family protein	Machinery gene
HHA37_RS23555 (HHA37_23590)	pilD	5092473..5093345 (+)	873	WP_023911898.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HHA37_RS23560 (HHA37_23595)	coaE	5093342..5093953 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
HHA37_RS23565 (HHA37_23600)	yacG	5093950..5094150 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HHA37_RS23570 (HHA37_23605)	-	5094187..5094396 (-)	210	WP_003094660.1	hypothetical protein	-
HHA37_RS23575 (HHA37_23610)	-	5094502..5095191 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HHA37_RS23580 (HHA37_23615)	-	5095188..5095658 (-)	471	WP_023911895.1	hypothetical protein	-
HHA37_RS23585 (HHA37_23620)	-	5095655..5096080 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HHA37_RS23590 (HHA37_23625)	-	5096213..5096842 (+)	630	WP_003120948.1	DUF1780 domain-containing protein	-
HHA37_RS23595 (HHA37_23630)	-	5096839..5097288 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HHA37_RS23600 (HHA37_23635)	-	5097314..5097484 (+)	171	WP_003094672.1	DUF3094 family protein	-
HHA37_RS23605 (HHA37_23640)	-	5097548..5098855 (+)	1308	WP_003125206.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62384.31 Da
• Isoelectric Point: 6.0138

>NTDB_id=439719 HHA37_RS23545 WP_122359259.1 5089549..5091249(+) (pilB) [Pseudomonas aeruginosa strain AA2]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAKQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=439719 HHA37_RS23545 WP_122359259.1 5089549..5091249(+) (pilB) [Pseudomonas aeruginosa strain AA2]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCAAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.692	100	0.583
pilB	Acinetobacter baylyi ADP1	57.371	99.47	0.571
pilB	Legionella pneumophila strain ERS1305867	55.3	100	0.553
pilB	Vibrio parahaemolyticus RIMD 2210633	51.241	99.647	0.511
pilF	Neisseria gonorrhoeae MS11	50.712	99.293	0.504
pilB	Vibrio cholerae strain A1552	50.265	99.823	0.502
pilB	Vibrio campbellii strain DS40M4	50	99.647	0.498
pilF	Thermus thermophilus HB27	39.292	99.823	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.607	93.11	0.378
pilB/pilB1	Synechocystis sp. PCC 6803	38.563	93.463	0.36