Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HHA37_RS23555 Genome accession   NZ_CP051547
Coordinates   5092473..5093345 (+) Length   290 a.a.
NCBI ID   WP_023911898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5091253..5099953 5092473..5093345 within 0


Gene organization within MGE regions


Location: 5091253..5099953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHA37_RS23550 (HHA37_23585) pilC 5091253..5092470 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  HHA37_RS23555 (HHA37_23590) pilD 5092473..5093345 (+) 873 WP_023911898.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HHA37_RS23560 (HHA37_23595) coaE 5093342..5093953 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HHA37_RS23565 (HHA37_23600) yacG 5093950..5094150 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HHA37_RS23570 (HHA37_23605) - 5094187..5094396 (-) 210 WP_003094660.1 hypothetical protein -
  HHA37_RS23575 (HHA37_23610) - 5094502..5095191 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  HHA37_RS23580 (HHA37_23615) - 5095188..5095658 (-) 471 WP_023911895.1 hypothetical protein -
  HHA37_RS23585 (HHA37_23620) - 5095655..5096080 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  HHA37_RS23590 (HHA37_23625) - 5096213..5096842 (+) 630 WP_003120948.1 DUF1780 domain-containing protein -
  HHA37_RS23595 (HHA37_23630) - 5096839..5097288 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  HHA37_RS23600 (HHA37_23635) - 5097314..5097484 (+) 171 WP_003094672.1 DUF3094 family protein -
  HHA37_RS23605 (HHA37_23640) - 5097548..5098855 (+) 1308 WP_003125206.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31897.92 Da        Isoelectric Point: 7.2968

>NTDB_id=439721 HHA37_RS23555 WP_023911898.1 5092473..5093345(+) (pilD) [Pseudomonas aeruginosa strain AA2]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGYEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=439721 HHA37_RS23555 WP_023911898.1 5092473..5093345(+) (pilD) [Pseudomonas aeruginosa strain AA2]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCTATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCTACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.017

99.655

0.548
  pilD Vibrio campbellii strain DS40M4

53.497

98.621

0.528
  pilD Acinetobacter nosocomialis M2

50.352

97.931

0.493
  pilD Acinetobacter baumannii D1279779

50.352

97.931

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472