Detailed information NTG0043742

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSF113_RS46940
• Genome accession: NC_016830
• Coordinates: 3851151..3852269 (+)
• Length: 372 a.a.
• NCBI ID: WP_014338692.1
• Uniprot ID: -
• Organism: Pseudomonas ogarae
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3846151..3857269

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSF113_RS46925 (PSF113_3266)	-	3847055..3847972 (+)	918	WP_014338690.1	sugar kinase	-
PSF113_RS46930 (PSF113_3267)	-	3848157..3848432 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
PSF113_RS46935 (PSF113_3268)	-	3848481..3851018 (-)	2538	WP_014338691.1	PAS domain-containing protein	-
PSF113_RS46940 (PSF113_3269)	pilU	3851151..3852269 (+)	1119	WP_014338692.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSF113_RS46945 (PSF113_3270)	-	3852437..3854392 (-)	1956	WP_014338693.1	acetoacetate--CoA ligase	-
PSF113_RS46950 (PSF113_3271)	-	3854551..3855327 (-)	777	WP_014338694.1	3-hydroxybutyrate dehydrogenase	-
PSF113_RS46955 (PSF113_3272)	-	3855335..3856726 (-)	1392	WP_014338695.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41411.42 Da
• Isoelectric Point: 6.3540

>NTDB_id=43742 PSF113_RS46940 WP_014338692.1 3851151..3852269(+) (pilU) [Pseudomonas ogarae]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVIKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDHAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHTESGAAAPPVDPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=43742 PSF113_RS46940 WP_014338692.1 3851151..3852269(+) (pilU) [Pseudomonas ogarae]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGGGCGCCACCGTG
CGCGCGTTTCGAGGGCGTGATCAAGCCCTTGGGGAACGAGGCGTTCAAGGTCGGCGACATTGCCCGGCTCGCCGAGTCCT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCTGGTGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCCCGGGTGCTGTTGGACACCATCATGCAGAAACAGGGGTTGATGCTGTTTGTCGGCGCGACAGGGT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
GGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGCGACCGGGAAACCATGGAACATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACCTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTTCCGGAAGAACGTCGGCCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGTACCCGCACCGGCCAGCGGCGGGCGGCGGTGGAGGTCATGCTGGGCTCGCCGACGGTGGCGGATCTGATCC
GGCGCAACGAGCTCGGCGAGCTCAAGGGCATCATGGAGAAGTCAGAGGAGTTGGGGATGCAAACCTTCGATCACGCGCTG
TTCAACCTGGTGGTCGAAGGCGCCATCGATGAGGAAGAAGCGCTGAAAAATGCCGATTCGGCGAACAACCTGCGGTTGCG
GTTGAAGTTGCATACCGAGTCGGGGGCGGCGGCGCCACCGGTCGATCCGGCGGCGGGTGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.43	0.54
pilU	Acinetobacter baylyi ADP1	51.648	97.849	0.505
pilU	Vibrio cholerae strain A1552	52.571	94.086	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.525	98.387	0.379
pilT	Vibrio cholerae strain A1552	40.841	89.516	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	89.516	0.366
pilT	Pseudomonas stutzeri DSM 10701	39.244	92.473	0.363
pilT	Pseudomonas aeruginosa PAK	38.953	92.473	0.36