Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HEP75_RS06615 Genome accession   NZ_CP051261
Coordinates   1579721..1580851 (+) Length   376 a.a.
NCBI ID   WP_185815706.1    Uniprot ID   A0A7G7SUL6
Organism   Xanthomonas sp. SI     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1574721..1585851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP75_RS06590 (HEP75_01289) - 1575017..1575472 (-) 456 WP_185825878.1 DUF4426 domain-containing protein -
  HEP75_RS06595 (HEP75_01290) proC 1575489..1576337 (-) 849 WP_185826519.1 pyrroline-5-carboxylate reductase -
  HEP75_RS06600 (HEP75_01291) - 1576483..1577511 (-) 1029 WP_185826520.1 hypothetical protein -
  HEP75_RS06605 (HEP75_01292) - 1577809..1578495 (-) 687 WP_185825879.1 YggS family pyridoxal phosphate-dependent enzyme -
  HEP75_RS06610 (HEP75_01293) pilT 1578584..1579621 (+) 1038 WP_185815705.1 type IV pilus twitching motility protein PilT Machinery gene
  HEP75_RS06615 (HEP75_01294) pilU 1579721..1580851 (+) 1131 WP_185815706.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HEP75_RS06620 (HEP75_01295) - 1581018..1582277 (+) 1260 WP_255424085.1 PQQ-binding-like beta-propeller repeat protein -
  HEP75_RS06625 (HEP75_01296) - 1582274..1583770 (+) 1497 WP_185826521.1 diguanylate cyclase -
  HEP75_RS06630 (HEP75_01297) - 1583881..1584423 (+) 543 WP_255424020.1 MFS transporter permease -
  HEP75_RS06635 (HEP75_01298) - 1584549..1585265 (+) 717 WP_185825882.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41932.26 Da        Isoelectric Point: 6.8811

>NTDB_id=437136 HEP75_RS06615 WP_185815706.1 1579721..1580851(+) (pilU) [Xanthomonas sp. SI]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=437136 HEP75_RS06615 WP_185815706.1 1579721..1580851(+) (pilU) [Xanthomonas sp. SI]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACTCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGTAACCAGGTCGGCATGGTGCTGCGCAGGATCGAGACGCGCATCCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGTTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACATGCGCGGCGTGGT
CGCCCAGCAACTGATCCCGACCCCGGACGGCAAGGGCCGGCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAAGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGCATGGACGGGGTGGAGATCGCGG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7SUL6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.583

98.138

0.644
  pilU Acinetobacter baylyi ADP1

63.305

94.947

0.601
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.286

93.085

0.394
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367