Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HGD77_RS13995 Genome accession   NZ_CP051208
Coordinates   2892437..2894146 (+) Length   569 a.a.
NCBI ID   WP_168730880.1    Uniprot ID   -
Organism   Acinetobacter sp. NEB149     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2887437..2899146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGD77_RS13950 (HGD77_13955) nusA 2887913..2889397 (-) 1485 WP_004281105.1 transcription termination factor NusA -
  HGD77_RS13955 (HGD77_13960) rimP 2889432..2889956 (-) 525 WP_004281103.1 ribosome maturation factor RimP -
  HGD77_RS13985 (HGD77_13990) secG 2891025..2891354 (-) 330 WP_004281101.1 preprotein translocase subunit SecG -
  HGD77_RS13990 (HGD77_13995) tpiA 2891368..2892159 (-) 792 WP_168730879.1 triose-phosphate isomerase -
  HGD77_RS13995 (HGD77_14000) pilB 2892437..2894146 (+) 1710 WP_168730880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HGD77_RS14000 (HGD77_14005) pilC 2894178..2895407 (+) 1230 WP_168730881.1 type II secretion system F family protein Machinery gene
  HGD77_RS17175 (HGD77_14010) pilD 2895407..2896267 (+) 861 WP_168730882.1 prepilin peptidase Machinery gene
  HGD77_RS14010 (HGD77_14015) coaE 2896269..2896868 (+) 600 WP_168730883.1 dephospho-CoA kinase -
  HGD77_RS14015 (HGD77_14020) - 2896890..2897816 (-) 927 WP_168730884.1 DMT family transporter -
  HGD77_RS14020 (HGD77_14025) rlmB 2897816..2898568 (-) 753 WP_168730885.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  HGD77_RS14025 (HGD77_14030) - 2898705..2899028 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63524.71 Da        Isoelectric Point: 4.7892

>NTDB_id=436954 HGD77_RS13995 WP_168730880.1 2892437..2894146(+) (pilB) [Acinetobacter sp. NEB149]
MTALQGSPRFTGFIRRLIEEGVISAEDMRSALAHAKQEKVDIVAELINQQQLSPTIIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKYRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEEFD
LDVDVESADPNKEDDESNKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKDLFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIVMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=436954 HGD77_RS13995 WP_168730880.1 2892437..2894146(+) (pilB) [Acinetobacter sp. NEB149]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGATCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCGTTGGCGCATGCCAAACAAGAAAAAGTCGATATTGTGGCTGAGTTAATTAACCAGCAGCAGCTTTCTC
CGACCATCATTGCTGAAACGATTTCAGTTGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCCCAAATT
TTGCGGGATGCGATTGATGAAAAGCTGATTACCAAGTATCGCATCCTGCCGATTTTTAAAAATTCCAGTATTTTATATGT
TGCGACCAGCAACCCGACCAATATCGAAGCGATCGATGCAGTCCGTTTTTCGACTAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACTGAAGAAAGCACGTTTGAGTTTGATGAAGAATTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGATGATGAAAGCAATAAAGGTGATGAAGCACCAATTGTCAA
ATATATCAATAAGTTACTCATCGATGCGATTCGTATGGGTGCTTCCGATCTACACTTTGAACCTTATGAAAAAATCTATC
GGGTACGGTACCGGGTTGATGGGGTGCTACGCCAGATTGCAACACCACCACTACAGCTGGCAAATCGCTTGTCTTCGCGT
CTTAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGGGTACCGCAAGATGGTCGTATTAAATTAAAACTTTCTAAAAA
TAAAGCCATTGATTTCCGTGTCAACTCTCTCCCTACCCTGTTTGGTGAGAAGCTGGTACTGCGGATTCTGGATCCCTCCA
GCGCGATGTTGGGAATTGATGCACTAGGTTATGAACCGGAACAAAAAGACCTTTTTATGCAAGCGCTGGACAAACCGCAA
GGCATGCTCCTGATCACTGGTCCAACAGGTTCCGGTAAAACCGTTTCACTTTATACCGGTTTGAATATCCTGAATCGTGA
AGATACCAATATTTCCACAGCGGAAGATCCGGTCGAGATTAACTTGCAAGGGATTAATCAGGTCAACGTTAATAATAAAG
TTGGCCTGACCTTCTCTGCCGCACTGAAGTCCTTCTTACGTCAAGACCCGGATATCGTCATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCTGCGCAGACCGGTCATATGGTGATGTCGACGCTGCATACCAATAGTGCGCC
TGAGACTTTGACCCGTTTACGCAATATGGGGGTGCCTTCTTTCAATATTGCCACTTCAGTCAACCTGGTCATTGCACAAC
GGTTGGCACGTCGTTTGTGTTCACAATGTAAAAAACCTGCCGATATTCCGAAACAAAGCTTACTGGAGATGGGTTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAGTGTCGCGAAGGCTATAAAGGTCG
TGTCGGTATTTATGAAGTTATGAAAGTAACGCCCGAAATTTCCAGAATTGTTATGGAGGACGGTAATGCACTTGAAATTG
CCGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.805
  pilB Acinetobacter baylyi ADP1

76.842

100

0.77
  pilB Legionella pneumophila strain ERS1305867

52.222

94.903

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.016

98.243

0.482
  pilF Neisseria gonorrhoeae MS11

48.841

98.594

0.482
  pilB Vibrio campbellii strain DS40M4

48.294

97.891

0.473
  pilB Vibrio cholerae strain A1552

50.87

90.861

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.747

100

0.374
  pilF Thermus thermophilus HB27

37.345

99.297

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.424

100

0.367