Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HEP74_RS18005 Genome accession   NZ_CP051190
Coordinates   4290192..4291340 (+) Length   382 a.a.
NCBI ID   WP_185814023.1    Uniprot ID   A0A7G7T172
Organism   Xanthomonas sp. SS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4285192..4296340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEP74_RS17980 (HEP74_03550) - 4285393..4286589 (-) 1197 WP_185814020.1 cystathionine gamma-synthase -
  HEP74_RS17985 (HEP74_03551) - 4286672..4288042 (-) 1371 WP_185814021.1 pyridoxal-phosphate dependent enzyme -
  HEP74_RS17990 (HEP74_03552) - 4288245..4288469 (-) 225 WP_009575526.1 YdcH family protein -
  HEP74_RS17995 (HEP74_03553) - 4288807..4289685 (-) 879 WP_185816693.1 OmpA family protein -
  HEP74_RS18000 (HEP74_03554) - 4289700..4290092 (-) 393 WP_185814022.1 DUF4398 domain-containing protein -
  HEP74_RS18005 (HEP74_03555) pilU 4290192..4291340 (+) 1149 WP_185814023.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HEP74_RS18010 (HEP74_03556) maiA 4291441..4292100 (-) 660 WP_185814024.1 maleylacetoacetate isomerase -
  HEP74_RS18015 (HEP74_03557) - 4292190..4293179 (-) 990 WP_185814025.1 fumarylacetoacetate hydrolase family protein -
  HEP74_RS18020 (HEP74_03558) - 4293556..4294866 (+) 1311 Protein_3546 DEAD/DEAH box helicase -
  HEP74_RS18025 (HEP74_03559) queD 4295046..4295405 (+) 360 WP_185814027.1 6-carboxytetrahydropterin synthase QueD -
  HEP74_RS18030 (HEP74_03560) - 4295686..4296057 (+) 372 WP_185814028.1 phasin family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42245.53 Da        Isoelectric Point: 6.4456

>NTDB_id=436763 HEP74_RS18005 WP_185814023.1 4290192..4291340(+) (pilU) [Xanthomonas sp. SS]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKDKNERRRPATEVLLNTPMIRDLLRRGQVHEIKAAMEESLEEGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYDAGGSGGASSPRITHGFG

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=436763 HEP74_RS18005 WP_185814023.1 4290192..4291340(+) (pilU) [Xanthomonas sp. SS]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACGGGAGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTGAACATGGCCATCGCGCTGCAGGACGCCGGCCGC
TTCCGGGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAACTGCACCTGCCGCAGGTGCTGAAGGACGTGATCATGACCCCGCGCGGGCTGGTCCTGGTGGTCGGCTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATT
CTCAACTTCTTCCCGGAAAGCGCGCACAAAAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCTCGCAGCG
CCTGGTCAAGGACAAGAACGAGCGCCGCCGCCCGGCCACCGAGGTGCTGCTGAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTCCACGAGATCAAGGCGGCCATGGAGGAATCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGCAGGGCCAGATCGAACAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCTCCAGCGGCGAACACGACCCTTACGCCGACTATGACGCCGGCGGCAGCGGCGGCGCCAGCT
CGCCGCGGATCACCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7T172

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

93.979

0.482
  pilU Vibrio cholerae strain A1552

47.619

98.953

0.471
  pilU Acinetobacter baylyi ADP1

49.153

92.67

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.531

93.717

0.427
  pilT Pseudomonas aeruginosa PAK

42.012

88.482

0.372
  pilT Acinetobacter baumannii D1279779

40.828

88.482

0.361
  pilT Acinetobacter baumannii strain A118

40.828

88.482

0.361
  pilT Acinetobacter nosocomialis M2

40.828

88.482

0.361