Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HF908_RS15300 Genome accession   NZ_CP051171
Coordinates   3236705..3237211 (-) Length   168 a.a.
NCBI ID   WP_193026451.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3231705..3242211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS15280 (HF908_15405) - 3231966..3232553 (+) 588 WP_193026448.1 DUF2946 family protein -
  HF908_RS15285 (HF908_15410) - 3232568..3234256 (-) 1689 WP_193026449.1 tetratricopeptide repeat protein -
  HF908_RS15290 (HF908_15415) moaC 3234350..3234826 (+) 477 WP_193026450.1 cyclic pyranopterin monophosphate synthase MoaC -
  HF908_RS15295 (HF908_15420) - 3234898..3236631 (-) 1734 WP_193028552.1 PglL family O-oligosaccharyltransferase -
  HF908_RS15300 (HF908_15425) pilA 3236705..3237211 (-) 507 WP_193026451.1 pilin Machinery gene
  HF908_RS15305 (HF908_15430) pilA 3237484..3238005 (-) 522 WP_193026452.1 pilin Machinery gene
  HF908_RS15310 (HF908_15435) - 3238199..3238897 (-) 699 WP_193026453.1 TerC family protein -
  HF908_RS15315 (HF908_15440) sucD 3239047..3239928 (-) 882 WP_193026454.1 succinate--CoA ligase subunit alpha -
  HF908_RS15320 (HF908_15445) sucC 3240014..3241180 (-) 1167 WP_193026455.1 ADP-forming succinate--CoA ligase subunit beta -
  HF908_RS15325 (HF908_15450) - 3241247..3241873 (-) 627 WP_193026456.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16971.66 Da        Isoelectric Point: 9.9490

>NTDB_id=436563 HF908_RS15300 WP_193026451.1 3236705..3237211(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2279]
MKSMRLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQTDLSVGSSVFTP
TKNVANLAIDGTTAGVPGQITVTYTTAAGGGTLALVPTAAGAKLPTGSAPSGPIMWTCYAAGKATAASSVNPNATMTLAA
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=436563 HF908_RS15300 WP_193026451.1 3236705..3237211(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2279]
ATGAAGTCGATGCGTCTCAACAAGCGTGTCCAAAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGTCGG
TATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGCCTGTCGCTGGCTG
CGCAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAAACTGACCTGTCGGTAGGTTCGTCGGTGTTCACGCCC
ACCAAGAACGTTGCAAACCTTGCAATTGATGGCACTACGGCTGGTGTTCCGGGCCAGATCACCGTGACCTATACGACGGC
AGCCGGCGGCGGCACGCTGGCTCTGGTCCCGACCGCGGCAGGCGCTAAGCTGCCGACGGGTTCGGCTCCGTCGGGTCCGA
TCATGTGGACCTGCTACGCTGCGGGTAAGGCTACGGCCGCCAGCAGCGTTAATCCGAACGCTACGATGACGCTGGCAGCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

89.349

100

0.899
  pilA2 Legionella pneumophila str. Paris

51.235

96.429

0.494
  pilA2 Legionella pneumophila strain ERS1305867

50.617

96.429

0.488
  comP Acinetobacter baylyi ADP1

44.97

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

41.176

91.071

0.375
  pilA/pilAI Pseudomonas stutzeri DSM 10701

34.659

100

0.363