Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HF908_RS15305 Genome accession   NZ_CP051171
Coordinates   3237484..3238005 (-) Length   173 a.a.
NCBI ID   WP_193026452.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2279     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3232484..3243005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF908_RS15285 (HF908_15410) - 3232568..3234256 (-) 1689 WP_193026449.1 tetratricopeptide repeat protein -
  HF908_RS15290 (HF908_15415) moaC 3234350..3234826 (+) 477 WP_193026450.1 cyclic pyranopterin monophosphate synthase MoaC -
  HF908_RS15295 (HF908_15420) - 3234898..3236631 (-) 1734 WP_193028552.1 PglL family O-oligosaccharyltransferase -
  HF908_RS15300 (HF908_15425) pilA 3236705..3237211 (-) 507 WP_193026451.1 pilin Machinery gene
  HF908_RS15305 (HF908_15430) pilA 3237484..3238005 (-) 522 WP_193026452.1 pilin Machinery gene
  HF908_RS15310 (HF908_15435) - 3238199..3238897 (-) 699 WP_193026453.1 TerC family protein -
  HF908_RS15315 (HF908_15440) sucD 3239047..3239928 (-) 882 WP_193026454.1 succinate--CoA ligase subunit alpha -
  HF908_RS15320 (HF908_15445) sucC 3240014..3241180 (-) 1167 WP_193026455.1 ADP-forming succinate--CoA ligase subunit beta -
  HF908_RS15325 (HF908_15450) - 3241247..3241873 (-) 627 WP_193026456.1 DUF2889 domain-containing protein -
  HF908_RS15330 (HF908_15455) recX 3242149..3242628 (-) 480 WP_193026457.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17888.55 Da        Isoelectric Point: 9.8138

>NTDB_id=436564 HF908_RS15305 WP_193026452.1 3237484..3238005(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2279]
MASKHSRNRWRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVVEGLSLAAQAKALVVENAANAQSSLSLGSASL
PASRNVSALNIDSSTGEIAVVYASAVTGAEANTLVLTPYSGSSSSLANLQAGSAPPTMVLWVCTAAGKSMPVNTVTQRTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=436564 HF908_RS15305 WP_193026452.1 3237484..3238005(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2279]
ATGGCATCGAAGCACAGCAGGAACAGGTGGCGCGGCCAGCGCGGCTTTACATTGATTGAATTGATGATCGTGGTGGCGAT
CATCGGGATATTGGCGGCGATTGCGATTCCGGCCTATCAGGACTACACGATCCGCGCGCGCGTGGTTGAGGGGCTGTCGC
TGGCAGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCTGCCAACGCGCAGAGTAGCCTGTCACTCGGCTCTGCATCGTTG
CCGGCCAGCAGGAACGTCAGTGCGCTGAATATTGATTCGTCCACGGGCGAAATCGCGGTGGTCTATGCATCTGCTGTTAC
GGGAGCGGAGGCGAATACGTTGGTATTGACCCCGTACTCCGGAAGCTCGTCATCACTTGCCAATCTGCAGGCGGGTTCAG
CGCCGCCGACAATGGTGCTGTGGGTGTGTACAGCCGCCGGCAAGTCGATGCCAGTCAATACTGTCACCCAGAGAACGACG
GCTACGCTGCCACCGAAGTACGCGCCCGCAGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

53.977

100

0.549
  pilA2 Legionella pneumophila str. Paris

46.061

95.376

0.439
  pilA2 Legionella pneumophila strain ERS1305867

45.455

95.376

0.434
  comP Acinetobacter baylyi ADP1

43.478

93.064

0.405
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.602

100

0.393