Detailed information NTG0001476

Experimentally validated

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: GTF74_RS00100
• Genome accession: NZ_CP047303
• Coordinates: 22657..24180 (+)
• Length: 507 a.a.
• NCBI ID: WP_000521788.1
• Uniprot ID: Q9KVV6
• Organism: Vibrio cholerae O1 biovar El Tor strain E7946
• Function: promote branch migration; interact with DprA; integration of tDNA Homologous recombination

Function

In vivo, we found that ComM was required for efficient comigration of linked genetic markers in Vibrio cholerae and Acinetobacter baylyi. In vitro, we showed that purified ComM binds ssDNA, oligomerizes into a hexameric ring, and has bidirectional helicase and branch migration activity. Using Helicobacter pylori and Vibrio cholerae as model systems, it was demonstrated that ComM directly interacts with the DprA winged-helix domain, and that this interaction is critical for DprA to recruit ComM to the recombination site to promote branch migration during NT.

Genomic Context

Location: 17657..29180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GTF74_RS00085 (GTF74_00085)	ilvE	19231..20169 (-)	939	WP_000201999.1	branched-chain-amino-acid transaminase	-
GTF74_RS00090 (GTF74_00090)	ilvM	20181..20465 (-)	285	WP_001190200.1	acetolactate synthase 2 small subunit	-
GTF74_RS00095 (GTF74_00095)	ilvG	20477..22123 (-)	1647	WP_000145255.1	acetolactate synthase 2 catalytic subunit	-
GTF74_RS00100 (GTF74_00100)	comM	22657..24180 (+)	1524	WP_000521788.1	YifB family Mg chelatase-like AAA ATPase	Machinery gene
GTF74_RS00105 (GTF74_00105)	-	24270..25154 (+)	885	WP_000881907.1	acyltransferase	-
GTF74_RS00110 (GTF74_00110)	-	25232..25834 (-)	603	WP_000732279.1	thiol:disulfide interchange protein DsbA/DsbL	-
GTF74_RS00115 (GTF74_00115)	-	25865..26851 (-)	987	WP_000189620.1	serine/threonine protein kinase	-
GTF74_RS00120 (GTF74_00120)	ccoG	26899..28323 (-)	1425	WP_000076153.1	cytochrome c oxidase accessory protein CcoG	-
GTF74_RS00125 (GTF74_00125)	-	28740..29006 (-)	267	WP_000649654.1	YihD family protein	-

Sequence

Protein

• Length: 507 a.a.
• Molecular weight: 55077.16 Da
• Isoelectric Point: 8.3602

>NTDB_id=1476 GTF74_RS00100 WP_000521788.1 22657..24180(+) (comM) [Vibrio cholerae O1 biovar El Tor strain E7946]
MGLAIIHSRASIGVQAPPVTVEVHISNGMPGFTLVGLPETTVKESRDRVRSAIINSRFEFPAKRITVNLAPADLPKEGGR
FDLPIALGILAASDQIARNKLESYEFIGELALSGEIRGVKGVLPAALAANQVERCLVVPHSNGDQAALVGVERHKSAQSL
LEVCADLCGQQTLSLFQSSPSVQQVSQTRDLQDIIGQQQGKRALEIAAAGNHNLLFLGPPGTGKTMLASRLCDLLPEMSD
EEAMETASIASLTQQEINQHNWKLRPFRAPHHSSSMAALVGGGTIPRPGEISLAHNGLLFLDEMPEFERKVLDSLREPLE
SGEIVISRAQGKTRFPARFQLVGALNPSPTGYYEGSQARANPQSILRYLSRLSGPLLDRFDMSIEIPALPKGTLANGGDR
GESTAAVRQRVWVARERMLARSGKVNALLQSREIEQYCPLLKADAEFLESALHRLGLSIRAYHRIIKVARTIADLQGEAQ
IARPHLAEALGYRAMDRLLKQLSAQNV

Nucleotide

• Length: 1524 bp

>NTDB_id=1476 GTF74_RS00100 WP_000521788.1 22657..24180(+) (comM) [Vibrio cholerae O1 biovar El Tor strain E7946]
ATGGGACTTGCGATCATTCATAGCCGCGCCAGTATTGGCGTGCAGGCACCTCCGGTCACGGTTGAGGTTCACATCAGTAA
CGGGATGCCGGGGTTTACCTTGGTAGGATTGCCAGAAACCACAGTAAAAGAGTCGCGCGATCGGGTGCGCAGCGCGATCA
TCAATTCTCGCTTTGAGTTTCCAGCGAAGCGCATTACGGTCAATTTAGCGCCAGCCGATTTACCCAAAGAGGGGGGACGT
TTTGATTTGCCGATTGCACTTGGCATTTTGGCGGCCTCGGATCAAATTGCGCGAAATAAGCTCGAAAGTTACGAATTTAT
CGGCGAATTGGCGCTATCGGGCGAAATTCGTGGCGTGAAAGGGGTTTTGCCTGCCGCGCTCGCGGCTAACCAAGTGGAGC
GCTGTTTGGTGGTGCCGCACAGTAATGGTGATCAGGCGGCTTTGGTCGGGGTTGAGCGGCATAAGTCGGCGCAAAGCTTA
CTCGAAGTGTGCGCAGACTTGTGTGGTCAGCAAACTCTATCTTTGTTTCAAAGTTCGCCCAGTGTGCAACAAGTAAGTCA
GACCCGCGATCTGCAAGACATCATAGGTCAGCAGCAGGGAAAACGAGCTTTGGAAATCGCTGCGGCTGGCAATCACAACC
TATTGTTTCTCGGCCCTCCGGGGACGGGTAAAACCATGCTCGCTTCACGGCTGTGCGATTTACTGCCAGAAATGAGTGAT
GAAGAGGCAATGGAAACAGCTTCGATTGCCTCTCTTACGCAGCAAGAGATTAACCAACATAACTGGAAGTTACGCCCTTT
TCGTGCACCGCACCATTCAAGCTCAATGGCGGCTTTGGTCGGGGGCGGCACCATTCCGCGCCCCGGTGAAATTTCTCTCG
CCCATAATGGACTACTGTTTTTGGACGAAATGCCGGAGTTTGAGCGTAAAGTGCTTGATTCACTGCGTGAACCGCTGGAA
TCGGGTGAGATCGTGATTTCGCGCGCGCAGGGGAAAACCCGTTTTCCAGCACGCTTTCAATTGGTCGGCGCGTTAAATCC
AAGCCCCACGGGCTACTACGAAGGCAGTCAGGCGCGTGCCAATCCGCAGAGTATTTTGCGCTATTTGAGTCGGCTTTCGG
GCCCTTTGTTGGATCGCTTTGATATGTCGATTGAGATCCCAGCGTTACCCAAAGGTACGCTCGCCAATGGGGGCGATCGT
GGTGAATCGACGGCGGCTGTGCGCCAGCGAGTGTGGGTAGCGCGTGAGCGTATGTTGGCGCGATCGGGCAAAGTGAATGC
TTTGCTGCAAAGTCGAGAAATTGAACAGTATTGCCCTTTGCTTAAAGCGGATGCGGAATTTCTAGAAAGCGCGCTGCATC
GCTTGGGTTTATCAATTCGGGCTTACCATCGCATCATTAAAGTGGCGCGAACCATAGCGGATTTGCAAGGTGAAGCGCAG
ATCGCTCGTCCACATTTGGCCGAGGCGTTGGGCTATCGAGCCATGGATCGCTTACTCAAACAGCTCAGTGCGCAGAACGT
CTAG

Domains

Predicted by InterProScan.

• Magnesium chelatase, subunit ChlI (Mg_chelatase) (190-391)

• Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C) (401-493)

• Subunit ChlI of Mg-chelatase (ChlI) (21-142)

3D structure

• AlphaFold DB: Q9KVV6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Vibrio cholerae strain A1552	100	100	1
comM	Vibrio campbellii strain DS40M4	85.01	100	0.85
comM	Haemophilus influenzae Rd KW20	67.258	100	0.673
comM	Glaesserella parasuis strain SC1401	64.147	100	0.653
comM	Legionella pneumophila strain ERS1305867	50.806	98.608	0.501
comM	Legionella pneumophila str. Paris	50.806	98.608	0.501
comM	Acinetobacter baylyi ADP1	49.194	100	0.493
RA0C_RS07335	Riemerella anatipestifer ATCC 11845 = DSM 15868	43.222	100	0.434

References

• [1]: Triana N Dalia et al. (2025) DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria. Proceedings of The National Academy of Sciences of The United States of America 122(15):e2421764122. [PMID: 40215278]
• [2]: Thomas M Nero et al. (2018) ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species. Nucleic Acids Research 46(12):6099-6111. [PMID: 29722872]