Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A3L25_RS28535 Genome accession   NZ_CP050951
Coordinates   6312682..6313950 (-) Length   422 a.a.
NCBI ID   WP_081237864.1    Uniprot ID   A0AAP9N4A7
Organism   Pseudomonas putida strain JQ581     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6307682..6318950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3L25_RS28520 (A3L25_028555) - 6309344..6310948 (-) 1605 WP_063423306.1 AAA family ATPase -
  A3L25_RS28525 (A3L25_028560) aroB 6310964..6312061 (-) 1098 WP_016502041.1 3-dehydroquinate synthase -
  A3L25_RS28530 (A3L25_028565) aroK 6312159..6312677 (-) 519 WP_024717571.1 shikimate kinase AroK -
  A3L25_RS28535 (A3L25_028570) pilQ 6312682..6313950 (-) 1269 WP_081237864.1 secretin N-terminal domain-containing protein Machinery gene
  A3L25_RS28540 (A3L25_028575) - 6313874..6314875 (-) 1002 WP_063423438.1 pilus assembly protein PilP -
  A3L25_RS28545 (A3L25_028580) - 6314887..6315423 (-) 537 WP_063423304.1 PilN domain-containing protein -
  A3L25_RS28550 (A3L25_028585) pilM 6315420..6316301 (-) 882 WP_063423303.1 pilus assembly protein PilM -
  A3L25_RS28555 (A3L25_028590) - 6316492..6318945 (+) 2454 WP_063423302.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45856.69 Da        Isoelectric Point: 4.9855

>NTDB_id=435756 A3L25_RS28535 WP_081237864.1 6312682..6313950(-) (pilQ) [Pseudomonas putida strain JQ581]
MKRVMKWMLAALLPIPLVSATPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDDVQGSVTLRLQDVPWDQALDLVLR
SKGLARQQEGNVLLVAPAAAFAPQPFDAHVGLLPDAQLQPLRRELIPIHHANAAELVELLMTSLADDSTLAVRGSLGVDE
RTNTLVAHQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGENLRPGKELFIDLGVERAGSSV
GLGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=435756 A3L25_RS28535 WP_081237864.1 6312682..6313950(-) (pilQ) [Pseudomonas putida strain JQ581]
ATGAAGAGAGTGATGAAATGGATGTTGGCAGCCCTGCTGCCGATCCCGTTGGTGTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGTTGAACTTCCAGGATGTGGAGGTGCGTTCGGTGCTGCAGGTGCTGGCCGATTATGCGGGCGTCAACCTGGTTG
CCAGCGATGACGTGCAGGGCAGCGTGACCTTGAGGTTGCAGGACGTGCCCTGGGACCAGGCCCTTGACCTGGTGCTGCGC
AGCAAGGGCCTTGCCCGGCAGCAAGAGGGCAATGTGCTGCTGGTGGCGCCTGCGGCAGCGTTTGCCCCGCAGCCGTTTGA
TGCCCATGTCGGCCTGTTGCCGGATGCACAATTGCAGCCGTTACGTCGTGAATTGATACCGATCCACCATGCCAACGCCG
CGGAACTGGTCGAGCTGTTGATGACCAGCCTGGCGGATGACAGCACACTCGCCGTTCGTGGCAGCCTGGGTGTCGATGAG
CGCACCAATACCCTGGTGGCGCACCAGCCCGCCGACCGACTGGCCGAGTTGCGACAACTGGTCGCGCAGCTGGATGTGCC
GGTGCGCCAGGTGGCGATCGAGGCACGTATCGTCGAGGCCAATGTCGACTACGAGAAAAGCCTGGGGGTGCGCTGGGGTG
GGCCGCTGTACGGTGAAAACCTGCGGCCGGGCAAGGAGTTGTTCATCGACCTCGGCGTGGAGCGGGCGGGCAGCAGTGTC
GGTCTGGGTCTGCTGCGCGGCGGCGTGCTGCTCGATCTGGAACTCAGCGCCATGGAAAAAAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTAAAAGGCACCGAGGTGCCCTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTCGCCTTTCGCGAAGCCTCGCTGTCGCTGGAGGTAACCCCGCAAATTACACCGGACAAC
AAGGTGATCATGGCGGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATTCGCAA
GAACGAGGTGAACGCCAAGGTTCGCGTGGCGGACGGTGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTAGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(50-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.542

100

0.609
  comQ Acinetobacter baylyi ADP1

41.778

100

0.446
  pilQ Acinetobacter baumannii D1279779

40.564

100

0.443
  pilQ Acinetobacter baumannii strain A118

40.564

100

0.443
  pilQ Legionella pneumophila strain Lp02

42.105

99.052

0.417
  pilQ Legionella pneumophila strain ERS1305867

42.105

99.052

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.33

99.763

0.412
  pilQ Vibrio cholerae strain A1552

41.33

99.763

0.412
  pilQ Vibrio campbellii strain DS40M4

39.583

100

0.405