Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HC167_RS02275 Genome accession   NZ_CP050790
Coordinates   467923..469629 (-) Length   568 a.a.
NCBI ID   WP_182787463.1    Uniprot ID   -
Organism   Aeromonas media strain FiBL-2097     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462923..474629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC167_RS02245 (HC167_02240) mutT 463522..463926 (+) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -
  HC167_RS02250 (HC167_02245) yacG 464002..464196 (-) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  HC167_RS02255 (HC167_02250) zapD 464206..464928 (-) 723 WP_404834478.1 cell division protein ZapD -
  HC167_RS02260 (HC167_02255) coaE 464966..465580 (-) 615 WP_182787457.1 dephospho-CoA kinase -
  HC167_RS02265 (HC167_02260) pilD 465599..466471 (-) 873 WP_182787459.1 A24 family peptidase Machinery gene
  HC167_RS02270 (HC167_02265) pilC 466552..467793 (-) 1242 WP_182787461.1 type II secretion system F family protein Machinery gene
  HC167_RS02275 (HC167_02270) pilB 467923..469629 (-) 1707 WP_182787463.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HC167_RS02280 (HC167_02275) tapA 469633..470070 (-) 438 WP_182788811.1 type IVa pilus major pilin TapA -
  HC167_RS02285 (HC167_02280) nadC 470395..471258 (-) 864 WP_182787465.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HC167_RS02290 (HC167_02285) - 471262..471723 (-) 462 WP_371316622.1 TIGR02281 family clan AA aspartic protease -
  HC167_RS02295 (HC167_02290) ampD 471869..472441 (+) 573 WP_182787467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HC167_RS02300 (HC167_02295) pdhR 472718..473482 (+) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62680.07 Da        Isoelectric Point: 5.8224

>NTDB_id=434778 HC167_RS02275 WP_182787463.1 467923..469629(-) (pilB) [Aeromonas media strain FiBL-2097]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=434778 HC167_RS02275 WP_182787463.1 467923..469629(-) (pilB) [Aeromonas media strain FiBL-2097]
ATGACCTCTAGCCCCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCAAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAACGACATCCTCGACAGCAAAGCTCTCG
CAGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACCGAGGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGATACCCTGGGCATGGATCACATCGATGAGGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAATTCCCGCCTCGACGACAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGAATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCTCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTCGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATCCTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGGATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.211

100

0.544
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.837

99.296

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

98.944

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37