Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HC169_RS19250 Genome accession   NZ_CP050788
Coordinates   4128045..4129751 (+) Length   568 a.a.
NCBI ID   WP_404839557.1    Uniprot ID   -
Organism   Aeromonas media strain FiBL-4359     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4123045..4134751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC169_RS19225 (HC169_19200) pdhR 4124264..4125028 (-) 765 WP_106885661.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HC169_RS19230 (HC169_19205) ampD 4125304..4125876 (-) 573 WP_404839555.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HC169_RS19235 (HC169_19210) - 4126013..4126483 (+) 471 WP_106885659.1 TIGR02281 family clan AA aspartic protease -
  HC169_RS19240 (HC169_19215) nadC 4126487..4127350 (+) 864 WP_394293767.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HC169_RS19245 (HC169_19220) - 4127663..4128037 (+) 375 WP_404839556.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HC169_RS19250 (HC169_19225) pilB 4128045..4129751 (+) 1707 WP_404839557.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HC169_RS19255 (HC169_19230) pilC 4129882..4131123 (+) 1242 WP_106885656.1 type II secretion system F family protein Machinery gene
  HC169_RS19260 (HC169_19235) pilD 4131204..4132076 (+) 873 WP_404839558.1 prepilin peptidase Machinery gene
  HC169_RS19265 (HC169_19240) coaE 4132095..4132709 (+) 615 WP_404839559.1 dephospho-CoA kinase -
  HC169_RS19270 (HC169_19245) zapD 4132747..4133469 (+) 723 WP_307765796.1 cell division protein ZapD -
  HC169_RS19275 (HC169_19250) yacG 4133482..4133676 (+) 195 WP_042053535.1 DNA gyrase inhibitor YacG -
  HC169_RS19280 (HC169_19255) mutT 4133751..4134155 (-) 405 WP_404839560.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62534.90 Da        Isoelectric Point: 5.8194

>NTDB_id=434738 HC169_RS19250 WP_404839557.1 4128045..4129751(+) (pilB) [Aeromonas media strain FiBL-4359]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLTEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPDAELQELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=434738 HC169_RS19250 WP_404839557.1 4128045..4129751(+) (pilB) [Aeromonas media strain FiBL-4359]
ATGACCTCTAGCCCCAATAGCGGCCTGGCTACCAGCTTGGCCGCCTCTTCCCTTCTCAGTGAGCCAGACTCACAGCGTTA
TCTGAGCCAAGCAAAGGCGCAGCGCAAACCCTTTGTCACCTTCCTGATCGAGAACGACATCCTCGACAGCAAGGCGCTGG
CTGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGACCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAGCACCATGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACTGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATC
TCGGAGCTCGAGGTTTCCGATGAGGGTTCGCGCCTCGACGAGAGCGTCAATACCGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCTATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGACTCGATATCGCCGAGCGACGCCTGCCCCAGGATGGGCGGATCAAGCTGAAGATCTCACGCAG
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGTGAGAAGATAGTCATCCGCTTGCTGGACTCGTCAG
CCGCCCGCCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGCTCTGGCAAGACGGTGTCGCTCTATACAGGTCTCAACATTCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CTGGGCTTACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAAATACGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACTCGGCAGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAAC
GTCTCGCCCGCAAGCTGTGCGATCACTGCAAGGCCCCCGAGGTGGTGCCCGACGCCGAACTGCAAGAGCTGGGCTTTACC
CAACAGCAGCTGGCTGCCGGACTGCGGCTGTTCAAGCCGGTCGGCTGCAAGGAGTGCTCGGGAGGATACAGAGGGCGGGT
CGGCATCTATGAAATCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGAGCGAACTCGCTGCAAATCGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACGCTACGCATCTCCGGCCTGGAGAAAGCCCGTTTGGGAGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.203

99.824

0.551
  pilB Acinetobacter baylyi ADP1

54.833

100

0.549
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

52.931

99.12

0.525
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

49.822

98.944

0.493
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.087

97.535

0.371
  pilF Thermus thermophilus HB27

39.962

92.958

0.371