Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HC170_RS19535 Genome accession   NZ_CP050786
Coordinates   4172019..4173725 (+) Length   568 a.a.
NCBI ID   WP_042649025.1    Uniprot ID   -
Organism   Aeromonas media strain DSM 4881     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4167019..4178725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC170_RS19500 (HC170_19375) ampD 4167443..4168015 (-) 573 WP_042648209.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HC170_RS19505 (HC170_19380) - 4168161..4168622 (+) 462 WP_371316622.1 TIGR02281 family clan AA aspartic protease -
  HC170_RS19510 (HC170_19385) nadC 4168626..4169489 (+) 864 WP_042648138.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HC170_RS19515 (HC170_19390) - 4169757..4170665 (-) 909 WP_404840787.1 RNA-guided endonuclease TnpB family protein -
  HC170_RS19520 - 4170646..4170897 (-) 252 WP_404840788.1 helix-turn-helix domain-containing protein -
  HC170_RS19525 (HC170_19395) tnpA 4170956..4171381 (+) 426 WP_041205574.1 IS200/IS605-like element ISAeme8 family transposase -
  HC170_RS19530 (HC170_19400) - 4171554..4172015 (+) 462 WP_082030008.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HC170_RS19535 (HC170_19405) pilB 4172019..4173725 (+) 1707 WP_042649025.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  HC170_RS19540 (HC170_19410) pilC 4173855..4175096 (+) 1242 WP_042649026.1 type II secretion system F family protein Machinery gene
  HC170_RS19545 (HC170_19415) pilD 4175177..4176049 (+) 873 WP_042649027.1 A24 family peptidase Machinery gene
  HC170_RS19550 (HC170_19420) coaE 4176068..4176682 (+) 615 WP_042649028.1 dephospho-CoA kinase -
  HC170_RS19555 (HC170_19425) zapD 4176720..4177442 (+) 723 WP_042649029.1 cell division protein ZapD -
  HC170_RS19560 (HC170_19430) yacG 4177452..4177646 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  HC170_RS19565 (HC170_19435) mutT 4177722..4178126 (-) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62696.11 Da        Isoelectric Point: 5.8224

>NTDB_id=434710 HC170_RS19535 WP_042649025.1 4172019..4173725(+) (pilB) [Aeromonas media strain DSM 4881]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDNVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=434710 HC170_RS19535 WP_042649025.1 4172019..4173725(+) (pilB) [Aeromonas media strain DSM 4881]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAATGACATCCTCGACAGCAAGGCCCTAG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTTATCGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCTCTGGAGGACTTTGGCTTCAGCTTCGGTCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAAATC
TCGGAACTCGAGGTCTCTGATGAGAGTTCGCGGCTCGACGACAACGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCACGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTTGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.473

99.648

0.523
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37