Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HB761_RS10515 Genome accession   NZ_CP050467
Coordinates   2224299..2225984 (-) Length   561 a.a.
NCBI ID   WP_255933887.1    Uniprot ID   A0AAE9N0B4
Organism   Vibrio campbellii strain LJC014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219299..2230984
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB761_RS10485 (HB761_10475) rplS 2219768..2220121 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  HB761_RS10490 (HB761_10480) yacG 2220459..2220659 (-) 201 WP_012128882.1 DNA gyrase inhibitor YacG -
  HB761_RS10495 (HB761_10485) zapD 2220716..2221456 (-) 741 WP_005534051.1 cell division protein ZapD -
  HB761_RS10500 (HB761_10490) coaE 2221484..2222098 (-) 615 WP_255899274.1 dephospho-CoA kinase -
  HB761_RS10505 (HB761_10495) pilD 2222099..2222968 (-) 870 WP_255899277.1 A24 family peptidase Machinery gene
  HB761_RS10510 (HB761_10500) pilC 2223041..2224264 (-) 1224 WP_255933885.1 type II secretion system F family protein Machinery gene
  HB761_RS10515 (HB761_10505) pilB 2224299..2225984 (-) 1686 WP_255933887.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HB761_RS10520 (HB761_10510) - 2225984..2226445 (-) 462 WP_255933889.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HB761_RS10525 (HB761_10515) nadC 2226710..2227597 (-) 888 WP_255933891.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HB761_RS10530 (HB761_10520) ampD 2227690..2228241 (+) 552 WP_255933893.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HB761_RS10535 (HB761_10525) pdhR 2228646..2229413 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62111.86 Da        Isoelectric Point: 5.0310

>NTDB_id=433071 HB761_RS10515 WP_255933887.1 2224299..2225984(-) (pilB) [Vibrio campbellii strain LJC014]
MLTSLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANDMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=433071 HB761_RS10515 WP_255933887.1 2224299..2225984(-) (pilB) [Vibrio campbellii strain LJC014]
ATGCTAACCAGCCTTTCAACGGTTCTTCGCCAAGCAGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCACGAATTGACAGAACACCTAA
GCTCAGTATTCGGGCTTTCTTGCACTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACGCTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACCTCTTCAACTTTGATTCTCGCGGTTGCTGACCCAACCAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCAACCGGCTTGCAAGTCGAGCTTGTCCTCGCTGACTTTCGCGAGCTGACAGCAG
CCATTCGGCGTTTGTATGGCCGATCGCTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGACGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAACCTTACGAAAATATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTGTTTGGAGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGTATTCTTAATAAGCCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTCGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTCCGCTCTTTCTTACGTCAAGATCCTGATGTCGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTACACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAACGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
GTTTGACGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGATA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

98.93

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

90.374

100

0.904
  pilB Vibrio cholerae strain A1552

73.31

100

0.734
  pilB Acinetobacter baumannii D1279779

51.434

93.226

0.48
  pilB Legionella pneumophila strain ERS1305867

47.576

99.287

0.472
  pilB Acinetobacter baylyi ADP1

50.097

92.157

0.462
  pilF Neisseria gonorrhoeae MS11

43.44

100

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.655

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

99.465

0.362