Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HBK86_RS16435 Genome accession   NZ_CP050385
Coordinates   3366056..3366541 (-) Length   161 a.a.
NCBI ID   WP_000993726.1    Uniprot ID   A0A242SN80
Organism   Acinetobacter baumannii strain VB82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3361056..3371541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBK86_RS16415 (HBK86_16415) - 3362713..3363096 (+) 384 WP_000090000.1 RidA family protein -
  HBK86_RS16420 (HBK86_16420) - 3363306..3363500 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  HBK86_RS16425 (HBK86_16425) bfr 3363746..3364210 (+) 465 WP_000678123.1 bacterioferritin -
  HBK86_RS16430 (HBK86_16430) - 3364252..3365880 (-) 1629 WP_002015646.1 PglL family O-oligosaccharyltransferase -
  HBK86_RS16435 (HBK86_16435) pilA 3366056..3366541 (-) 486 WP_000993726.1 pilin Machinery gene
  HBK86_RS16440 (HBK86_16440) - 3366883..3367443 (-) 561 WP_000258950.1 TPM domain-containing protein -
  HBK86_RS16445 (HBK86_16445) - 3367437..3368519 (-) 1083 WP_001246384.1 TPM domain-containing protein -
  HBK86_RS16450 (HBK86_16450) - 3368541..3369131 (-) 591 WP_000846931.1 LemA family protein -
  HBK86_RS16455 (HBK86_16455) - 3369261..3370148 (-) 888 WP_001017328.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16591.91 Da        Isoelectric Point: 5.0937

>NTDB_id=432084 HBK86_RS16435 WP_000993726.1 3366056..3366541(-) (pilA) [Acinetobacter baumannii strain VB82]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVGRSNVAAAVQTLTSNKSGLESYVMEFGEFPDGTTAPAAGDPTANPP
VAATRGERPQDLGIVNTTLGSIALGDKNNGAGIITLTFATGNPGIKGKKVQLARDANGTWTCVTDVDEKFAAKACPVGTP
Q

Nucleotide


Download         Length: 486 bp        

>NTDB_id=432084 HBK86_RS16435 WP_000993726.1 3366056..3366541(-) (pilA) [Acinetobacter baumannii strain VB82]
ATGAATGCACAAAAAGGTTTTACGTTAATTGAACTCATGATCGTGGTTGCCATTATCGGTATCTTGGCAGCAATTGCGAT
TCCGCAATATCAAAATTATGTGGGTCGCTCTAATGTAGCAGCAGCTGTCCAAACACTTACATCAAATAAATCTGGTTTAG
AAAGTTATGTAATGGAATTTGGTGAATTCCCTGACGGTACTACGGCTCCAGCTGCAGGAGATCCAACAGCGAACCCACCA
GTAGCTGCTACTCGAGGTGAGCGACCACAGGATTTAGGTATTGTTAATACAACATTAGGAAGTATTGCTTTAGGTGATAA
AAACAATGGAGCTGGCATTATCACTTTAACATTTGCGACAGGCAATCCTGGTATTAAAGGCAAAAAGGTTCAGTTAGCTC
GTGATGCAAATGGAACTTGGACATGTGTAACTGATGTTGATGAGAAATTTGCAGCCAAAGCTTGTCCAGTAGGAACACCA
CAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A242SN80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.75

99.379

0.484
  pilA Pseudomonas aeruginosa PAK

46.541

98.758

0.46
  pilA Vibrio cholerae C6706

45.86

97.516

0.447
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.86

97.516

0.447
  pilA Vibrio cholerae strain A1552

45.86

97.516

0.447
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.558

95.652

0.398
  pilE Neisseria gonorrhoeae MS11

37.736

98.758

0.373
  comP Acinetobacter baylyi ADP1

36.709

98.137

0.36