Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   HBA47_RS02720 Genome accession   NZ_CP050136
Coordinates   512773..514041 (+) Length   422 a.a.
NCBI ID   WP_019389681.1    Uniprot ID   -
Organism   Kingella kingae strain ATCC 23332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507773..519041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA47_RS02695 (HBA47_02705) pgeF 508125..508904 (-) 780 WP_154699893.1 peptidoglycan editing factor PgeF -
  HBA47_RS02700 (HBA47_02710) cyaY 509054..509377 (-) 324 WP_099046165.1 iron donor protein CyaY -
  HBA47_RS02705 (HBA47_02715) - 509468..509629 (+) 162 WP_003787775.1 lipoprotein -
  HBA47_RS02710 (HBA47_02720) lysA 509626..510855 (+) 1230 WP_154699894.1 diaminopimelate decarboxylase -
  HBA47_RS02715 (HBA47_02725) pilF 511041..512726 (+) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA47_RS02720 (HBA47_02730) pilG 512773..514041 (+) 1269 WP_019389681.1 type II secretion system F family protein Machinery gene
  HBA47_RS02725 (HBA47_02735) pilD 514113..514952 (+) 840 WP_341785704.1 A24 family peptidase Machinery gene
  HBA47_RS02730 (HBA47_02740) coaE 514954..515562 (+) 609 WP_003787784.1 dephospho-CoA kinase -
  HBA47_RS02735 (HBA47_02745) zapD 515677..516438 (+) 762 WP_019389683.1 cell division protein ZapD -
  HBA47_RS02740 (HBA47_02750) pncC 516458..516934 (+) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  HBA47_RS02745 (HBA47_02755) - 516944..517564 (+) 621 WP_019389684.1 L-threonylcarbamoyladenylate synthase -
  HBA47_RS02750 (HBA47_02760) - 517641..518834 (-) 1194 WP_038307267.1 acetate kinase -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46031.51 Da        Isoelectric Point: 10.1438

>NTDB_id=430285 HBA47_RS02720 WP_019389681.1 512773..514041(+) (pilG) [Kingella kingae strain ATCC 23332]
MAAKKPVKKAPVKKAAAAKPVKKEKGNRYQFEGRNLSTDSIVRGEVVAKNEDEARQKLVRRQIKVLQLTKMKKAKDKKIT
SADIAVFTRQLSTMMKAGLPLMQAFDIVAKGHSNASMTAMLMEIRNDVEQGEALGDSFAKHPKYFDKFYCNLVAAGEAGG
VLEGLLDKLATYKEKTEAIKKKVKSALTYPISIVVVAVVLVIVMMMFVLPEFGKVYESMGAKLPPLTQFMMDISNAFVQF
GWLIIAGLIGGVVALIQFHKRSPKFQKRVDAMLLKLPVFGDIVRKATIARWARTTATLFTAGVPLVEVLDSVAGAAGNII
YEEATREIRSKVNQGISLTSGMQATNLFPNMVVQMASIGEESGALDDMLNKTAEFYEDEVDAAVAMLSSLMEPIIMVVLG
GIIGTILVAMYLPLFNLGNVVG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=430285 HBA47_RS02720 WP_019389681.1 512773..514041(+) (pilG) [Kingella kingae strain ATCC 23332]
ATGGCTGCAAAAAAACCTGTTAAAAAAGCACCTGTAAAAAAAGCAGCGGCTGCCAAGCCTGTTAAAAAAGAAAAAGGCAA
TCGCTACCAGTTTGAAGGTCGTAATTTAAGTACCGATTCAATCGTACGCGGCGAAGTGGTTGCCAAAAATGAAGACGAAG
CACGTCAGAAGTTAGTGCGTCGCCAAATTAAGGTTTTGCAACTGACCAAAATGAAAAAGGCAAAAGATAAGAAAATTACT
TCTGCCGACATTGCGGTGTTTACGCGCCAACTTTCTACCATGATGAAAGCAGGTTTGCCATTGATGCAAGCGTTTGATAT
CGTGGCAAAAGGTCATTCCAATGCATCTATGACAGCCATGCTGATGGAAATTCGCAACGATGTGGAACAAGGTGAAGCAT
TGGGCGATTCGTTTGCCAAACACCCTAAATATTTTGACAAATTCTATTGTAACTTGGTTGCCGCAGGTGAAGCAGGCGGT
GTATTGGAAGGCTTGTTAGACAAACTGGCAACCTATAAAGAAAAAACCGAAGCTATTAAGAAAAAAGTGAAATCGGCTTT
GACTTACCCAATTTCTATTGTGGTAGTGGCAGTCGTTTTGGTAATTGTGATGATGATGTTTGTATTGCCTGAGTTTGGTA
AGGTGTATGAAAGCATGGGGGCAAAATTGCCACCTCTCACACAGTTTATGATGGATATTTCTAATGCATTTGTTCAATTT
GGTTGGTTGATTATTGCAGGCTTGATTGGTGGAGTAGTTGCATTGATTCAATTTCATAAAAGATCACCTAAGTTCCAAAA
AAGAGTAGATGCTATGTTGTTAAAATTACCTGTATTTGGTGATATTGTCCGTAAAGCCACGATTGCACGTTGGGCACGTA
CAACCGCCACTTTGTTTACAGCGGGTGTACCATTGGTGGAAGTATTGGATTCGGTAGCAGGTGCCGCGGGTAATATTATT
TATGAAGAGGCCACTCGTGAAATTCGTTCCAAAGTAAACCAAGGTATTTCTTTGACATCGGGTATGCAGGCAACCAATTT
GTTCCCAAACATGGTAGTGCAAATGGCTTCCATTGGTGAAGAATCTGGCGCGTTGGACGATATGTTAAACAAAACTGCCG
AATTCTATGAAGACGAAGTCGATGCTGCGGTAGCCATGCTGTCGTCTTTGATGGAACCCATCATTATGGTGGTATTGGGT
GGCATTATTGGTACGATTCTGGTTGCGATGTACTTACCATTGTTCAACTTGGGTAACGTGGTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

68

94.787

0.645
  pilG Neisseria meningitidis 44/76-A

67.25

94.787

0.637
  pilC Legionella pneumophila strain ERS1305867

48.995

94.313

0.462
  pilC Acinetobacter baylyi ADP1

45.337

91.469

0.415
  pilC Pseudomonas stutzeri DSM 10701

50.435

81.754

0.412
  pilC Acinetobacter baumannii D1279779

45.195

91.232

0.412
  pilC Vibrio cholerae strain A1552

37.26

98.578

0.367