Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HBA47_RS02715 Genome accession   NZ_CP050136
Coordinates   511041..512726 (+) Length   561 a.a.
NCBI ID   WP_003787777.1    Uniprot ID   F5S8J8
Organism   Kingella kingae strain ATCC 23332     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506041..517726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBA47_RS02690 (HBA47_02700) - 506475..508073 (+) 1599 WP_166503111.1 ABC-F family ATPase -
  HBA47_RS02695 (HBA47_02705) pgeF 508125..508904 (-) 780 WP_154699893.1 peptidoglycan editing factor PgeF -
  HBA47_RS02700 (HBA47_02710) cyaY 509054..509377 (-) 324 WP_099046165.1 iron donor protein CyaY -
  HBA47_RS02705 (HBA47_02715) - 509468..509629 (+) 162 WP_003787775.1 lipoprotein -
  HBA47_RS02710 (HBA47_02720) lysA 509626..510855 (+) 1230 WP_154699894.1 diaminopimelate decarboxylase -
  HBA47_RS02715 (HBA47_02725) pilF 511041..512726 (+) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HBA47_RS02720 (HBA47_02730) pilG 512773..514041 (+) 1269 WP_019389681.1 type II secretion system F family protein Machinery gene
  HBA47_RS02725 (HBA47_02735) pilD 514113..514952 (+) 840 WP_341785704.1 A24 family peptidase Machinery gene
  HBA47_RS02730 (HBA47_02740) coaE 514954..515562 (+) 609 WP_003787784.1 dephospho-CoA kinase -
  HBA47_RS02735 (HBA47_02745) zapD 515677..516438 (+) 762 WP_019389683.1 cell division protein ZapD -
  HBA47_RS02740 (HBA47_02750) pncC 516458..516934 (+) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  HBA47_RS02745 (HBA47_02755) - 516944..517564 (+) 621 WP_019389684.1 L-threonylcarbamoyladenylate synthase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62226.13 Da        Isoelectric Point: 5.1186

>NTDB_id=430284 HBA47_RS02715 WP_003787777.1 511041..512726(+) (pilF) [Kingella kingae strain ATCC 23332]
MSAGLLRILVQKKLVPQENLAKYQEAIKADKPILASIFADKLITPEDLAELCATMFNYPMLDLNFFPRSKIVPDILDESQ
MMELRCIPLFKRGKKLYLAVSDPTNIQNLQKVVFSSGLAIDLVIVRDDQLNTVLEWFGQSNSSLLKEMGMDSSLQEAQAQ
TGMIVDGEEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGQLREIVQPPLALRSQLASRIKVMAKLDIS
EKRVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKKLIMDAIYRPYGMVLVTGPT
GSGKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPENVLRDAGFTDEDLAKDW
TMYRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGVTTLEEVIATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=430284 HBA47_RS02715 WP_003787777.1 511041..512726(+) (pilF) [Kingella kingae strain ATCC 23332]
ATGAGCGCAGGTTTATTGCGAATTTTGGTACAGAAAAAACTCGTACCACAAGAGAATTTAGCCAAATATCAAGAAGCGAT
TAAAGCCGATAAACCGATTTTGGCTTCCATTTTTGCCGACAAGCTGATTACACCAGAAGACTTGGCAGAGCTGTGTGCCA
CCATGTTTAACTATCCGATGTTGGATTTAAACTTCTTCCCACGCAGCAAAATCGTCCCTGATATTTTGGATGAATCGCAA
ATGATGGAATTGCGCTGCATTCCATTGTTTAAGCGTGGTAAAAAATTGTATTTGGCGGTGTCTGACCCAACCAATATCCA
AAATTTGCAAAAAGTCGTGTTCAGCAGCGGCTTGGCGATTGATTTAGTGATTGTGCGCGATGATCAATTAAATACCGTGT
TGGAATGGTTTGGTCAAAGCAATAGCAGCCTGCTTAAAGAAATGGGCATGGACAGCAGCCTGCAAGAAGCACAAGCCCAA
ACAGGCATGATTGTGGATGGCGAAGAAGAAGAGGACGGCCCTGTTGCCCGGTTCATTCAAAAAATTCTGCACGATGCCGT
GACATCGGGTGCGTCCGATATTCACTTTGAGTTCTATGAATTTATGGCGCGTGTGCGTTTCCGTACAGACGGTCAGTTGC
GCGAAATCGTACAGCCACCATTGGCATTGCGTAGCCAGCTGGCATCGCGCATCAAGGTAATGGCGAAACTGGATATTTCT
GAAAAGCGCGTCCCACAAGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACAC
CATGCCGTGTTTGTTTGGCGAAAAAGTCGTGATGCGTATTTTGAACTCCGATGCCGCCAGCTTGAACATTGACCAACTGG
GTTTTGAAGACTTCCAGAAAAAATTGATTATGGACGCGATTTATCGTCCATACGGCATGGTGCTGGTAACAGGGCCTACG
GGCTCGGGTAAAACCGTATCGCTTTACACCTGCTTAAACATTTTGAATACCGATGACGTAAACATCTCCACCGCTGAAGA
CCCTGCGGAGATTAACTTACCAGGCATCAATCAAGTAAACGTAAACGACAAACAAGGCTTAACTTTTGAAGCTGCGCTAA
AAGCATTCTTGCGTCAAGACCCAGACATCATCATGATTGGTGAGATTCGTGACTTGGGTACAGCCGATATTGCGATTAAA
GCCGCACAAACAGGTCACATGGTGTTCTCAACCTTGCACACCAACAATGCCCCAGCCACCTTGTCGCGTATGTTGAACAT
GGGTGTTGCGCCGTTTAATATTGCATCGGCAGTGAACTTGATTATGGCGCAGCGTTTGGCACGTCGTTTGTGCAGCGCGT
GTAAAGCCCCAATGGAACGTCCGCCTGAAAATGTATTGCGCGATGCAGGTTTTACTGACGAAGATTTGGCAAAAGATTGG
ACAATGTATCGTGCCGTAGGTTGTGATGCGTGTAAAGGCAAAGGCTACAAAGGTCGTGCAGGCTTGTACGAAGTGATGCC
GATGACGGAAAAAATGAAAGCCGTTATCATGAAAGGCGGTACAGAAGTGGATATTGCTAACATTGCCTATGAAGAAGGTT
TGTGCGATTTGCGCCGTTCTGGCTTGCTCAAAGTGATGCAAGGTGTAACCACTTTGGAAGAAGTGATTGCGACAACCAAC
GAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(181-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S8J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.271

100

0.683
  pilB Acinetobacter baumannii D1279779

52.91

100

0.535
  pilB Acinetobacter baylyi ADP1

51.865

100

0.52
  pilB Legionella pneumophila strain ERS1305867

47.695

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.455
  pilB Vibrio campbellii strain DS40M4

45.421

95.365

0.433
  pilB Vibrio cholerae strain A1552

46.318

91.979

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

92.692

0.369