Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G8D99_RS13930 Genome accession   NZ_CP049916
Coordinates   3053131..3054840 (+) Length   569 a.a.
NCBI ID   WP_166326903.1    Uniprot ID   A0A6G8S739
Organism   Acinetobacter lanii strain 185     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3048131..3059840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D99_RS13890 (G8D99_13890) nusA 3048844..3050328 (-) 1485 WP_166326895.1 transcription termination factor NusA -
  G8D99_RS13895 (G8D99_13895) rimP 3050370..3050894 (-) 525 WP_166326897.1 ribosome maturation factor RimP -
  G8D99_RS13920 (G8D99_13920) secG 3051731..3052060 (-) 330 WP_166326899.1 preprotein translocase subunit SecG -
  G8D99_RS13925 (G8D99_13925) tpiA 3052073..3052864 (-) 792 WP_166326901.1 triose-phosphate isomerase -
  G8D99_RS13930 (G8D99_13930) pilB 3053131..3054840 (+) 1710 WP_166326903.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G8D99_RS13935 (G8D99_13935) pilC 3054866..3056092 (+) 1227 WP_166326905.1 type II secretion system F family protein Machinery gene
  G8D99_RS15820 (G8D99_13940) pilD 3056092..3056955 (+) 864 WP_166326907.1 A24 family peptidase Machinery gene
  G8D99_RS13945 (G8D99_13945) coaE 3056994..3057596 (+) 603 WP_166326909.1 dephospho-CoA kinase -
  G8D99_RS13950 (G8D99_13950) - 3057642..3058559 (-) 918 WP_166327793.1 DMT family transporter -
  G8D99_RS13955 (G8D99_13955) rlmB 3058610..3059359 (-) 750 WP_166326911.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  G8D99_RS13960 (G8D99_13960) - 3059502..3059825 (-) 324 WP_166326913.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63561.91 Da        Isoelectric Point: 5.2348

>NTDB_id=428649 G8D99_RS13930 WP_166326903.1 3053131..3054840(+) (pilB) [Acinetobacter lanii strain 185]
MSLEPHVPKFSGINRRLVDEGVLTAQQMQTAVESARKSQKKLIPYLIEHLNLKSLIIAELTAQEFGEPLFDLDAYNPEML
PKEIVDKNLVYRHQVVPLFQRGSLLYVATSDPTNMEALDAIRFNSKLNVHPIIVEQHKLEKLIEKHFQEETQFNFGDEDL
DVDVVDTDQNTNQEDDDPSGDDSPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIANPPLQLANRLASR
LKVMSQMDISEKRTPQDGRIKLKLSKTKAIDFRVNCLPTLFGEKLVLRILDPSSAMLGIDALGYEEAQKQLFMDALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPAEIPKNSLLEMGFT
EQDLADPEFQIYQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAQASARLGFDNLRRSGLKKVISGLTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=428649 G8D99_RS13930 WP_166326903.1 3053131..3054840(+) (pilB) [Acinetobacter lanii strain 185]
ATGAGTCTAGAACCACATGTACCAAAATTTTCGGGGATCAATCGTCGCTTGGTCGATGAAGGGGTTTTGACAGCCCAACA
AATGCAAACGGCTGTCGAAAGCGCAAGAAAAAGTCAAAAAAAATTAATTCCTTATTTAATTGAACATCTTAATCTTAAAT
CATTGATTATTGCTGAACTGACTGCACAAGAGTTTGGTGAACCACTGTTTGATCTCGATGCATACAATCCTGAGATGCTA
CCCAAAGAGATTGTCGATAAAAATTTGGTTTATCGCCACCAAGTAGTTCCGCTGTTTCAACGGGGTAGCCTGCTTTATGT
GGCCACCAGTGATCCAACCAATATGGAAGCCTTGGACGCGATTCGCTTTAACAGCAAGCTCAATGTGCATCCGATTATAG
TTGAACAGCATAAACTGGAAAAATTGATTGAAAAGCATTTTCAAGAAGAAACCCAGTTTAATTTTGGTGATGAAGATCTT
GATGTCGATGTCGTAGACACAGATCAAAATACCAATCAAGAAGATGACGATCCCTCAGGTGATGATTCACCGATCGTTAA
ATACATCAACAAACTTTTGGTCGATGCGATTCGAATGGGTGCATCGGACTTGCATTTTGAGCCTTATGAAAAATCCTATC
GAGTGCGTTATCGCGTCGATGGTGTGTTGCGTCATATTGCCAATCCCCCACTCCAATTGGCCAATCGTTTAGCCTCACGT
TTAAAAGTGATGTCACAAATGGACATTTCTGAAAAACGTACCCCGCAAGATGGTCGTATTAAACTCAAACTATCAAAAAC
CAAAGCCATTGATTTCCGTGTCAACTGCTTACCGACCTTATTCGGTGAAAAGTTAGTGCTGCGTATTCTCGATCCGTCTA
GTGCCATGCTGGGCATTGATGCACTCGGTTATGAAGAAGCGCAAAAACAACTGTTTATGGATGCGTTGGAAAAACCGCAA
GGCATGCTGTTAATCACAGGTCCAACCGGTTCAGGTAAAACGGTGTCCTTATATACCGGTCTAAATATCTTGAATACCGA
AGACACCAATATTTCGACCGCCGAAGATCCGGTAGAGATTAACTTGGAAGGGATTAATCAGGTCAACGTCAATCCTAAAG
TGGGACTGACCTTCTCTGCGGCATTAAAAGCCTTTCTACGTCAAGATCCAGATGTGATCATGGTCGGTGAGATTCGAGAC
TTAGAAACTGCTGAAATTGCGATTAAAGCGGCTCAAACCGGTCATATGGTGATGTCAACGCTGCATACCAACAGTGCGCC
TGAAACTTTAACCCGTTTACGTAACATGGGTGTGCCTTCGTTTAACATTGCCACTTCGGTCAATCTGGTCATTGCCCAGC
GTTTGGCACGACGCCTATGCAGTCACTGTAAAGCCCCTGCTGAGATTCCGAAAAACAGTTTGCTTGAAATGGGCTTTACC
GAACAAGACTTGGCTGATCCTGAATTTCAGATTTATCAACCGGTGGGGTGCCCTGAATGTCGTGAAGGCTATAAAGGCCG
TGTCGGGATTTATGAGGTCATGAAAGTCACGCCTGAAATTTCTAAAATTATTATGGAAGATGGCAATGCCCTTGAAATTG
CTCAAGCCTCAGCCAGATTGGGCTTTGATAATTTACGACGTTCAGGCTTAAAGAAAGTGATCAGTGGTTTAACTTCGCTC
CAAGAAGTCAATCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8S739

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.901

99.121

0.782
  pilB Acinetobacter baylyi ADP1

75.311

98.946

0.745
  pilB Legionella pneumophila strain ERS1305867

51.562

100

0.522
  pilF Neisseria gonorrhoeae MS11

48.841

98.594

0.482
  pilB Vibrio cholerae strain A1552

49.457

97.012

0.48
  pilB Vibrio campbellii strain DS40M4

49.367

97.188

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

48.49

98.946

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.333

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

35.065

100

0.38
  pilF Thermus thermophilus HB27

36.713

100

0.369